An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

Qin, Yi; Ge, Gaoran; Yang, Peng; Wang, Liangliang; Qiao, Yusen; Pan, Guoqing; Yang, Huilin; Bai, Jiaxiang; Cui, Wenguo; Geng, Dechun

doi:10.1002/advs.202207334

Cited by 61 publications

(14 citation statements)

References 252 publications

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“…ADSCs can differentiate in vitro and in vivo into osteogenic, chondrogenic, myogenic, epithelial, endothelial, and neuron-like cells in the presence of lineage-specific induction factors [ 31 ]. In addition, mechanical or electromagnetic stimulation, pharmaceuticals, and genetic reprogramming have been widely explored to promote the differentiation of ADSCs into specific cell lineages [ 32 , 33 ].…”

Section: Adsc Characteristicsmentioning

confidence: 99%

Adipose-derived stem cell-based optimization strategies for musculoskeletal regeneration: recent advances and perspectives

Yuan,

Song,

Jiang

et al. 2024

Stem Cell Res Ther

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Musculoskeletal disorders are the leading causes of physical disabilities worldwide. The poor self-repair capacity of musculoskeletal tissues and the absence of effective therapies have driven the development of novel bioengineering-based therapeutic approaches. Adipose-derived stem cell (ADSC)-based therapies are being explored as new regenerative strategies for the repair and regeneration of bone, cartilage, and tendon owing to the accessibility, multipotency, and active paracrine activity of ADSCs. In this review, recent advances in ADSCs and their optimization strategies, including ADSC-derived exosomes (ADSC-Exos), biomaterials, and genetic modifications, are summarized. Furthermore, the preclinical and clinical applications of ADSCs and ADSC-Exos, either alone or in combination with growth factors or biomaterials or in genetically modified forms, for bone, cartilage, and tendon regeneration are reviewed. ADSC-based optimization strategies hold promise for the management of multiple types of musculoskeletal injuries. The timely summary and highlights provided here could offer guidance for further investigations to accelerate the development and clinical application of ADSC-based therapies in musculoskeletal regeneration. Graphical abstract

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Section: Adsc Characteristicsmentioning

confidence: 99%

Adipose-derived stem cell-based optimization strategies for musculoskeletal regeneration: recent advances and perspectives

Yuan,

Song,

Jiang

et al. 2024

Stem Cell Res Ther

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“…Regenerative medicine is a treatment modality that restores function by regenerating tissues and organs lost due to accident or disease, and cell transplantation treatment using stem cells is a known form of this modality [ 35 ]. However, regenerative medicine requires a special cell culture facility, leading to higher medical costs, the risk of mutations occurring during cell culture, and slow supply speeds based on the time required for culture [ 36 , 37 , 38 ]. “Stem cells” is a general term for cells that have both self-renewal ability and multipotency and include somatic stem cells (adult stem cells, tissue stem cells), embryonic stem (ES) cells, induced pluripotent stem cells (iPSCs), etc.…”

Section: Mrna-based Protein Supplementation For Regenerative Medicinementioning

confidence: 99%

Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine

Inagaki

2023

JDB

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The COVID-19 pandemic generated interest in the medicinal applications of messenger RNA (mRNA). It is expected that mRNA will be applied, not only to vaccines, but also to regenerative medicine. The purity of mRNA is important for its medicinal applications. However, the current mRNA synthesis techniques exhibit problems, including the contamination of undesired 5′-uncapped mRNA and double-stranded RNA. Recently, our group developed a completely capped mRNA synthesis technology that contributes to the progress of mRNA research. The introduction of chemically modified nucleosides, such as N1-methylpseudouridine and 5-methylcytidine, has been reported by Karikó and Weissman, opening a path for the practical application of mRNA for vaccines and regenerative medicine. Yamanaka reported the production of induced pluripotent stem cells (iPSCs) by introducing four types of genes using a retrovirus vector. iPSCs are widely used for research on regenerative medicine and the preparation of disease models to screen new drug candidates. Among the Yamanaka factors, Klf4 and c-Myc are oncogenes, and there is a risk of tumor development if these are integrated into genomic DNA. Therefore, regenerative medicine using mRNA, which poses no risk of genome insertion, has attracted attention. In this review, the author summarizes techniques for synthesizing mRNA and its application in regenerative medicine.

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“…Adipose-derived stem cells (ADSCs) are commonly applied in the reconstruction and repair of soft tissues, playing a pivotal role in both plastic surgery and tissue engineering ( Dai et al, 2016 ; Wankhade et al, 2016 ; Gadelkarim et al, 2018 ). Numerous studies have demonstrated that during in vitro cultivation, ADSCs could secret a multitude of bioactive cytokines and growth factors, including transforming growth factor-β, hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) ( Qin et al, 2023 ; Wang et al, 2023 ; Wu et al, 2023 ). These mediators are instrumental in fostering cell proliferation, cellular migration, angiogenesis regulation, immune response modulation, and inflammation response regulation, assuming an indispensable role in the wound repair process ( Song Y. et al, 2023 ; Wu et al, 2023 ; Kang et al, 2024 ).…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived stem cells derived decellularized extracellular matrix enabled skin regeneration and remodeling

Zhang,

Xiang,

Yang

et al. 2024

Front. Bioeng. Biotechnol.

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The tissues or organs derived decellularized extracellular matrix carry immunogenicity and the risk of pathogen transmission, resulting in limited therapeutic effects. The cell derived dECM cultured in vitro can address these potential risks, but its impact on wound remodeling is still unclear. This study aimed to explore the role of decellularized extracellular matrix (dECM) extracted from adipose derived stem cells (ADSCs) in skin regeneration.Methods: ADSCs were extracted from human adipose tissue. Then we cultivated adipose-derived stem cell cells and decellularized ADSC-dECM for freeze-drying. Western blot (WB), enzyme-linked immunosorbent assay (ELISA) and mass spectrometry (MS) were conducted to analyzed the main protein components in ADSC-dECM. The cell counting assay (CCK-8) and scratch assay were used to explore the effects of different concentrations of ADSC-dECM on the proliferation and migration of human keratinocytes cells (HaCaT), human umbilical vein endothelia cells (HUVEC) and human fibroblasts (HFB), respectively. Moreover, we designed a novel ADSC-dECM-CMC patch which used carboxymethylcellulose (CMC) to load with ADSC-dECM; and we further investigated its effect on a mouse full thickness skin wound model.Results: ADSC-dECM was obtained after decellularization of in vitro cultured human ADSCs. Western blot, ELISA and mass spectrometry results showed that ADSC-dECM contained various bioactive molecules, including collagen, elastin, laminin, and various growth factors. CCK-8 and scratch assay showed that ADSC-dECM treatment could significantly promote the proliferation and migration of HaCaT, human umbilical vein endothelia cells, and human fibroblasts, respectively. To evaluate the therapeutic effect on wound healing in vivo, we developed a novel ADSC-dECM-CMC patch and transplanted it into a mouse full-thickness skin wound model. And we found that ADSC-dECM-CMC patch treatment significantly accelerated the wound closure with time. Further histology and immunohistochemistry indicated that ADSC-dECM-CMC patch could promote tissue regeneration, as confirmed via enhanced angiogenesis and high cell proliferative activity.Conclusion: In this study, we developed a novel ADSC-dECM-CMC patch containing multiple bioactive molecules and exhibiting good biocompatibility for skin reconstruction and regeneration. This patch provides a new approach for the use of adipose stem cells in skin tissue engineering.

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An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

Cited by 61 publications

References 252 publications

Adipose-derived stem cell-based optimization strategies for musculoskeletal regeneration: recent advances and perspectives

Adipose-derived stem cell-based optimization strategies for musculoskeletal regeneration: recent advances and perspectives

Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine

Adipose-derived stem cells derived decellularized extracellular matrix enabled skin regeneration and remodeling

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