An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans

Zhu, Suxian; Vossen, Jack H.; Bergervoet, Marjan; Nijenhuis, Maarten; Kodde, Linda; Kessel, G.J.T.; Vleeshouwers, Vivianne G. A. A.; Visser, Richard G. F.; Jacobsen, E.

doi:10.1007/s10681-014-1276-0

Cited by 45 publications

(34 citation statements)

References 42 publications

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“…The single substitution, R3b G3111 , was present in one variety and the double substitution, Rpi‐R3b G1696/G3111 , in 8 varieties including Innovator, Picasso and the late blight differential line 2573 (2). For the differential line 2573 (2), it has been shown previously that Rpi‐R3b function, such as the recognition of the corresponding effector from P. infestans, Avr3b, is maintained (Zhu et al ., ). Similarly, Avr3b is recognised in Picasso and a specific cell death response is observed in Nicotiana benthamiana upon co‐infiltration of Rpi‐R3b G1696/G3111 with Avr3b (Strachan et al., submitted).…”

Section: Resultsmentioning

confidence: 97%

Tracking disease resistance deployment in potato breeding by enrichment sequencing

Armstrong

Vossen

Lim

et al. 2018

Plant Biotechnology Journal

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Summary Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing ( dR enSeq) enables the high‐confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost‐effective in monitoring resistances than whole‐genome sequencing and can be used to appraise (trans) gene integrity efficiently. All currently known NB ‐ LRR s effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dR enSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.

show abstract

Section: Resultsmentioning

confidence: 97%

Tracking disease resistance deployment in potato breeding by enrichment sequencing

Armstrong

Vossen

Lim

et al. 2018

Plant Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…The single substitution, R3b G3111 , was present in one variety and the double substitution, Rpi-R3b G1696/G3111 , in 8 varieties including Innovator, Picasso and the late blight differential line 2573 (2). For the differential line 2573 (2), it has been shown previously that Rpi-R3b function, such as the recognition of the corresponding effector from P. infestans, Avr3b, is maintained (Zhu et al, 2014). Similar, Avr3b is recognised in Picasso and a strong cell death responds is observed in Nicotiana benthamiana upon co-infiltration of Rpi-R3b G1696/G3111 with Avr3b (Strachan et al, submitted).…”

Section: Drenseq Identifies Functional Nlrs and Appraises Transgene Imentioning

confidence: 89%

Tracking disease resistance deployment in potato breeding by enrichment sequencing

Armstrong

Vossen

Lim

et al. 2018

Preprint

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SummaryFollowing the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans)gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.

show abstract

“…Many problems of this kind have been solved by development of monogenic differential plants [21]. However, in this case the use of P. infestans races with known virulence genes will probably fail to provide the same R gene identification accuracy, as compared to their direct identification using molecular markers.…”

Section: Introductionmentioning

confidence: 99%

ANTICIPATORY BREEDING: MOLECULAR MARKERS AS A TOOL IN DEVELOPING DONORS OF POTATO (Solanum tuberosum L.) LATE BLIGHT RESISTANCE FROM COMPLEX INTERSPECIFIC HYBRIDS

Fadina¹,

Бекетова²,

Соколова³

et al. 2017

S-h. biol.

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An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans

Cited by 45 publications

References 42 publications

Tracking disease resistance deployment in potato breeding by enrichment sequencing

Tracking disease resistance deployment in potato breeding by enrichment sequencing

Tracking disease resistance deployment in potato breeding by enrichment sequencing

ANTICIPATORY BREEDING: MOLECULAR MARKERS AS A TOOL IN DEVELOPING DONORS OF POTATO (Solanum tuberosum L.) LATE BLIGHT RESISTANCE FROM COMPLEX INTERSPECIFIC HYBRIDS

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