An UPLC-MS/MS method for determination of solasonine in rat plasma and its application of a pharmacokinetic and bioavailability study

Chen, Yuanyuan; Zhang, Shuangshuang; Chen, Dahui; Zhou, Mi; Zheng, Jing; Xiang, Zheng

doi:10.1016/j.jchromb.2015.01.017

Cited by 13 publications

(6 citation statements)

References 13 publications

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“…In order to exclude the influences of sex as shown in References [17,18], only male mice were used in this study. In addition, thanks to the extremely low absolute bioavailability of oral administration [34], mice were administered with solasonine by intraperitoneal injection. According to Reference [25] and Horn’s assay ( n = 5, 2.15×) [33], the in vivo toxicity of solasonine was evaluated at the doses 10, 21.5, 46.4, and 100 mg/kg.…”

Section: Resultsmentioning

confidence: 99%

In Vivo Toxicity of Solasonine and Its Effects on cyp450 Family Gene Expression in the Livers of Male Mice from Four Strains

Zhong

Chen

et al. 2018

Toxins

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Solasonine was reported to inhibit tumour cell growth in several different models. The in vivo toxicity of solasonine, the effects of genetic background on its toxicity, and its possible roles in regulating the expression of cyp450 family genes were still unclear and required characterisation. Here, Horn’s assays were performed on male mice from four different strains, and the expression of cyp450 family genes in their livers was examined by RT-PCR and ELISA. Mice treated by intraperitoneal injection with high levels of solasonine showed immediate post-excitatory depression, intraperitoneal tissue adhesion, and dissolving of cells in the liver. Furthermore, these four mouse strains showed different toxicological sensitivity to solasonine. The strains, in decreasing order of LD50 value, rescuing speed of body weight, and more severe pathological symptoms, were KM, ICR, C57BL/6, and BALB/c. Interestingly, more cyp450 genes were downregulated at the mRNA and/or protein level in the livers of male mice from C57BL/6 or BALB/c strains than those from KM or ICR strains. These results suggest that (1) Solasonine has hepatic toxicity and downregulates cyp450 genes expression at transcriptional and/or post-transcriptional levels; (2) Genetic background is an important factor which can affect the in vivo toxicity; (3) Downregulation of cyp450 gene expression in the liver may be a clue to help understand whether or not a given strain is sensitive to solasonine; (4) Influences on the expression of cyp450 genes should be considered when using solasonine alone, or in combination with other drugs.

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Section: Resultsmentioning

confidence: 99%

In Vivo Toxicity of Solasonine and Its Effects on cyp450 Family Gene Expression in the Livers of Male Mice from Four Strains

Zhong

Chen

et al. 2018

Toxins

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“…A variety of sample preparation techniques have been applied in a bioanalytical method such as protein precipitation, liquid-liquid extraction, and solid-phase extraction [32]. Among these, the most widely used are protein precipitation and liquid-liquid extraction followed by solid-phase extraction which are used in different category such as antibiotic [33][34][35][36][37][38][39][40], anticancer [36,[41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62], antiviral [63][64][65][66][67], antifungal [68][69][70][71][72], cardiovascular [73][74][75][76][77]…”

Section: Application Of Uhplc-ms/msmentioning

confidence: 99%

Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations

et al. 2019

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Background: UHPLC-MS/MS is connected in various research facilities for the qualitative and quantitative investigation of a pharmaceutical substance, pharmaceutical items, and biological specimen. Main body: The commence review article is an endeavor to offer pervasive awareness around assorted aspects and details about the UHPLC-MS/MS and related techniques with the aim on practice to an estimation of medicinal active agents in the last 10 years. The article also focused on isolation, separation, and characterization of present impurity in drug and biological samples. Conclusion: Review article compiles a general overview of medicinally important drugs and their analysis with UHPLC-MS/MS. It gives fundamental thought regarding applications of UHPLC-MS/MS for the study on safety limit. The summary of developed UHPLC-MS/MS methods gives a contribution to the future trend and limitations in this area of research.

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“…8a). As shown in the figure, after the tail vein injection of drugs (single-dose samples), the time-concentration curve (TCC) of the solasonine in the lung is similar to the plasma drug metabolism feature in previous study (Chen et al, 2015). After intravenous injection, the drug quickly enters the lung tissue from the blood and reaches a peak at the time of about 5 min (Tmax).…”

Section: Lungsupporting

confidence: 71%

“…Previous studies (Chen et al, 2015), traditional LC-MS / MS methods were used to measure the concentration of solasonine in rat blood by i.v. The results showed that the solasonine distribution showed a rapid trend in absorption and elimination.…”

Section: Discussionmentioning

confidence: 99%

Quantitative MALDI-MSI combined with LC-MS/MS metabolomics analysis to study the inhibition of solasonine in lung cancer

Shi

Jin

et al.

Authorea

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Background and purpose: The Chinese medicine monomer solasonine has been shown to be an effective inhibitor of Lung adenocarcinoma in vitro and in vivo. The research on the application of solasonine in lung cancer mostly involves the cell level, the lack of information on the spatial distribution of drugs and related metabolic pathways are common problems faced by many Chinese medicine monomers. Experimental Approach: LC-MS/MS metabolomics analysis was performed to reveal the underlying regulatory mechanism, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) and 3D computational reconstruction were applied to illustrated the spatial-temporal distribution of solasonine. Solamargine was chosen as the internal standard to correct the calibration curve due to the similarity in structure. Key Results: Metabolomics analysis illustrated that solasonine promotes A549 cells ferroptosis via GPX4-induced destruction of the glutathione redox system. Detailed distribution features of solasonine in different organs were revealed by MALDI-MSI after intravenous administration in the mice. The heterogeneity of solasonine distribution and penetration in tumor demonstrated that significant drug deposits around the necrotic area. Conclusion and Implication: The anti-tumor mechanism of solasonine associated with ferroptosis is identified for the first time. It provides an additional basis for the previous conclusion that solasonine promotes tumor necrosis. Quantitative spatial-temporal information obtained here can improve our understanding of pharmacokinetics (PK), pharmacodynamics (PD), potential transient toxicities of solasonine in organs, and possibly direct further optimization of drug properties to reduce drug-induced organ toxicity and broaden the scope of application.

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An UPLC-MS/MS method for determination of solasonine in rat plasma and its application of a pharmacokinetic and bioavailability study

Cited by 13 publications

References 13 publications

In Vivo Toxicity of Solasonine and Its Effects on cyp450 Family Gene Expression in the Livers of Male Mice from Four Strains

In Vivo Toxicity of Solasonine and Its Effects on cyp450 Family Gene Expression in the Livers of Male Mice from Four Strains

Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations

Quantitative MALDI-MSI combined with LC-MS/MS metabolomics analysis to study the inhibition of solasonine in lung cancer

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