Anal Human Papillomavirus Testing with Digene's Hybrid Capture 2 Using Two Different Sampling Methods

Roka, Florian; Roka, Julia; Trost, Andreas; Schalk, Horst; Zagler, Christian; Kirnbauer, Reinhard; Salat, Andreas

doi:10.1007/s10350-007-9082-6

Cited by 17 publications

(11 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flocked swabs outperformed Dacron for cell count per slide based on slide imaging [ 29 ]. However, sample collection using Dacron swabs identifi ed more human papillomavirus-positive patients and yielded higher relative light unit values than using the cervical brush [ 42 ].…”

Section: Biomarkersmentioning

confidence: 95%

Anal Cytology

Darragh

Palefsky

2015

The Bethesda System for Reporting Cervical Cytology

View full text Add to dashboard Cite

Anal cytology was fi rst included in the 2001 Bethesda System Atlas. It has gained acceptance as a tool for anal cancer screening in conjunction with high-resolution anoscopy (HRA) and biopsy -in a role similar to the Pap test [ 1 -4 ]. Recommendations in TBS 2001 included guidance on sampling, adequacy, use of Bethesda terminology for anal cytology, and basic morphologic characteristics of anal squamous intraepithelial lesions (ASIL). This 2014 update to the chapter incorporates a brief review of the epidemiology of anal cancer, additional images, and expands information on the performance characteristics of anal cytology, the role of HPV testing and biomarkers, and briefl y addresses clinical management. Anal CancerAnal squamous cell carcinoma is an uncommon cancer. Over 90 % of anal cancers are attributable to persistent HPV infections with HPV16 predominating [ 5 ]. The 2014 American Cancer Society [ 6 ] estimates for anal cancer in the United States are approximately 7,210 new cases (4,550 in women and 2,660 in men) and 950 deaths (580 in women and 370 in men). However, rates of anal squamous cell carcinoma have been increasing over the last several decades, especially in high-risk groups.

show abstract

Section: Biomarkersmentioning

confidence: 95%

Anal Cytology

Darragh

Palefsky

2015

The Bethesda System for Reporting Cervical Cytology

View full text Add to dashboard Cite

show abstract

“…Cotton swabs should not be used as the cells are absorbed and do not release as easily into the cytology medium or onto the slide. Synthetic swabs have also been shown to provide better sampling for HPV DNA testing (Roka et al, 2008).…”

Section: Anal Cytology Collectionmentioning

confidence: 99%

Elements of an Anal Dysplasia Screening Program

Jay

2011

Journal of the Association of Nurses in AIDS Care

View full text Add to dashboard Cite

The incidence of anal cancer in HIV-infected men who have sex with men (MSM) is highly elevated compared to the general population, as is the incidence of its precursor lesion, high-grade anal intraepithelial neoplasia (HGAIN). MSM in general and other immunocompromised populations are also at higher risk. Treatment of HGAIN may prevent development of cancer, similar to the decrease in cervical cancers that has occurred since the advent of cervical cancer screening programs in women. Cervical cancer screening tools have been adapted and validated for screening, diagnosis, and treatment of anal HGAIN. Anal cancer screening programs have now been available for more than a decade, although they are not yet standards of care. Incorporating screening procedures into practice depends on the available resources in a particular community. This article discusses the procedures for anal cancer screening including cytology, digital anal rectal examinations, high-resolution anoscopy, and biopsy.

show abstract

“…Therefore, this was our choice for screening patients [13]. All of these findings suggest that HPV infection is important for the development of LAIN, HAIN and ASCUS, and that HIV-positivity increases the risk of disease in HPV-positive subjects [27].…”

Section: Discussionmentioning

confidence: 99%

“…The aim of the present study was to evaluate the influence of highrisk HPV DNA test on screening for anal lesions [13].…”

Section: Introductionmentioning

confidence: 99%

High-Risk HPV DNA Test for Screening Anal Lesions in HIV-Positive and Negative Patients

Cruzand¹

2014

J AIDS Clin Res

View full text Add to dashboard Cite

The high incidence of anal dysplasia is related to many factors. The infections caused by HPV and HIV seem to be determinants of the following anal Pap smear changes: atypical squamous cells of undetermined significance (ASCUS), low-grade anal intraepithelial neoplasia (LAIN) and high-grade anal intraepithelial neoplasia (HAIN). Highrisk HPV infection is confirmed by the HPV DNA test, using hybrid capture technology. Screening for anal lesions should be complemented with the anal Pap smear protocol and high-resolution anoscopy. The aim of this study was to evaluate the influence of the high-risk HPV DNA test on screening for anal lesions. We evaluated 70 HIV-positive and negative patients who had previously had anal intercourse, at CRT/AIDS São Paulo from January 2013 to December 2013. All of the patients underwent the high-risk HPV DNA test, anal Pap smear protocol and highresolution anoscopy. The anal lesions were treated with 90% trichloroacetic acid (TCA) and 5% imiquimod for 12 weeks. The statistical analysis was performed using hypothesis tests on proportions and the significance level was set at less than 5%. 31 HIV-positive male patients, 19 HIV-negative male patients and 20 HIV-negative female patients were evaluated. 28 HIV-positive patients were positive for the high-risk HPV DNA test and 29 patients were positive for the high-risk HPV DNA test and had anal dysplasia. Our conclusion is that it was more common for HIVpositive patients to be infected with oncogenic HPV and the oncogenic HPV was more frequent in patients with anal dysplasia.

show abstract

Anal Human Papillomavirus Testing with Digene's Hybrid Capture 2 Using Two Different Sampling Methods

Cited by 17 publications

References 21 publications

Anal Cytology

Anal Cytology

Elements of an Anal Dysplasia Screening Program

High-Risk HPV DNA Test for Screening Anal Lesions in HIV-Positive and Negative Patients

Contact Info

Product

Resources

About