Analgesic and antibutyrylcholinestrasic activities of the venom prepared from the Mediterranean jellyfish Pelagia noctiluca (Forsskal, 1775)

Abstract

BackgroundToxins derived from jellyfishes have been exploited as a model for the development of new drug promising applications to treat neurodegenerative diseases. The present work is aimed to evaluate the acute toxicity of crude venom of Pelagia noctiluca and then to screen the analgesic and antibutyrylcholinestrasic (anti-BuChE) activities of the crude venom and its fractions.MethodsSephadex G75 gel was used to separate crude venom of Pelagia noctiluca, which led to some fractions. In addition, in vivo analgesic and in vitro plasma antibutyrylcholinestrasic activities were carried out with Pelagia crude venom and its fractions respectively.ResultsThe crude venom and its fractions displayed analgesic and anti-BuChE activities at different doses without inducing acute toxicity. Fraction 2 possesses the highest analgesic and antibutyrylcholinestrasic properties. The crude venom and fraction 1 had shown to possess less significant inhibitory activity against analgesic and antibutyrylcholinestrasic models.ConclusionsBased on this study, the crude venom of Pelagia noctiluca is found to be a useful tool for probing pharmacological activity. The purification and the determination of chemical structures of compounds of active fractions of the venom are under investigation.

Ancient Venom Systems: A Review on Cnidaria Toxins

“…Recently antibutyrylcholinestrasic activity was detected in the crude venom extracted from the tentacle material of the Mediterranean jellyfish Pelagia noctiluca . Inhibition of butylcholinestrase in the central nervous system may prove useful in the treatment of neurodegenerative diseases such as Alzheimer’s disease and senile dementia [ 138 ].…”

Bioactive toxins from stinging jellyfish

“…The SDS-PAGE profiles of venom extracted from Mediterranean and Caribbean specimens (S anchez-Rodríguez and Lucio-Martínez, 2011;Ayed et al, 2012c;Maisano et al, 2013) revealed protein bands between 4 and 150 kDa, with the most intense bands at an apparent molecular weight higher than 80 kDa (S anchez-Rodríguez and Lucio-Martínez, 2011;Ayed et al, 2012c).…”

“…In a first study, the two main fractions were used to test the analgesic activity at low doses and the inhibition of butyrylcholineesterase activity. The latter could be linked to the neurotoxic effects of the venom (Ayed et al, 2012c). In a second study, four fractions were isolated (Ayed et al, 2012b).…”

Synthesis and Biological Activities of Novel 1,7-dihydropyrazolo[3,4-d]imidazo[1,2-f]pyrimidines

“…The analgesic activity was performed according to the method of Ayed et al [10]. Swiss mice (20-30 g) were selected one day prior to each test and were divided into six groups of six mice each.…”