Analgesic Effects and the Mechanisms of Anti-inflammation of Ergostatrien-3β-ol from Antrodia camphorata Submerged Whole Broth in Mice

Abstract

Ergostatrien-3β-ol (ST1), an active and major ingredient from Antrodia camphorata (AC) submerged whole broth was evaluated for the analgesic and anti-inflammatory effects. Treatment of male imprinting control region (ICR) mice with ST1 (1, 5, and 10 mg/kg) significantly inhibited the numbers of acetic-acid-induced writhing response in 10 min. Also, our result showed that ST1 (10 mg/kg) significantly inhibited the formalin-induced pain in the late phase (p < 0.001). In the anti-inflammatory test, ST1 (10 mg/kg) decreased the paw edema at 4 and 5 h after λ-carrageenin (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. We also demonstrated that ST1 significantly attenuated the malondialdehyde (MDA) level in the edema paw at 5 h after Carr injection. ST1 (1, 5, and 10 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at 5 h after Carr injection. Also, ST1 (5 and 10 mg/kg) diminished the serum tumor necrosis factor (TNF-R) at 5 h after Carr injection. Western blotting revealed that ST1 (10 mg/kg) decreased Carr-induced inducible nitric oxide synthase (iNOS), and cycloxyclase (COX-2) expressions at 5 h in the edema paw. An intraperitoneal (ip) injection treatment with ST1 also diminished neutrophil infiltration into sites of inflammation, as did indomethacin (Indo). The anti-inflammatory mechanisms of ST1 might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver through the suppression of TNF-R and NO.

Genomic and transcriptomic analyses of the medicinal fungusAntrodia cinnamomeafor its metabolite biosynthesis and sexual development

“…In contrast, A. cinnamommea is an endemic mushroom that is restricted to the endemic aromatic tree Cinnamomum kanehirai Hayata in Taiwan. The economic value of A. cinnamommea is mainly due to its medicinal use (8)(9)(10)(11). According to Ortiz-Santana et al (3), A. cinnamomea (T. camphorates) belongs to a clade that includes Taiwanofungus, Dacryobolus, and other related genera.…”

“…cinnamomea is rich in polysaccharides (4) and produces specific ergostane triterpenoids, including antcins C and K (5), zhankuic acids A, B, and C (6), and antrocamphin (7). A. cinnamomea has long been the most valued medicinal fungus in Taiwan, as a remedy for various diseases, including hypertension, abdominal pains, hepatitis B, and cancers (8)(9)(10)(11). Also, the extract of A. cinnamomea has long been used to ameliorate the effects of alcohol intoxication.…”

Knowledge Discovery of Suppressive Effect of Disease and Increased Anti-oxidative Function by Low-dose Radiation Using Self-organizing Map

“…Other reports showing the oxidative stress-related disease suppressive effect of antioxidants were col- and selectively extracted from these reports [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] ( Table 2). Dataset 2 had 122 records in 17 reports and 6% missing values.…”

“…10) Our results showed that doi: 10.3769/radioisotopes.67. 43 2 1 Datasets Dataset 1 was selectively extracted from our reports over the past five years. They show the suppressive effects on the oxidative stress-related diseases induced by low-dose radiation [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] (Table 1).…”

Efeito do tratamento quimioterápico sobre a ação da proteína quinase dependente de RNA (PKR) nos sistemas nociceptivo e muscular

“…Os neurônios do gânglios da raiz dorsal são considerados os principais alvos de ação da cisplatina, podendo causar danos no DNA nuclear e morte celular (Cavaletti et al, 1995;Carozzi et al, 2015) e (Huang et al, 2010). Embora o efeito da cisplatina em danos no DNA nuclear tenha sido amplamente investigado, sua interação com DNA mitocondrial foi apenas recentemente investigado (Podratz et al, 2011).…”