Análise computacional da compactação da cromatina de espermatozoides de galo

Rodrigues, Ana Carolina Nunes; Rocha, João Victor Ritinto da; Beletti, Marcelo Emı́lio

doi:10.1590/s0102-09352009000600008

Cited by 11 publications

(16 citation statements)

References 13 publications

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“…However, in contrast with the data from other species, there are still only few studies of rooster semen; thus, there is still no reliable parameters for analysis (LUCASZEWICZ et al, 2008;ARRUDA et al, 2011). On the other hand, the semen of old roosters has been observed to have higher degree of chromatin changes than that of young roosters, which may decrease their fertility, even in the absence of changes in the semen physical characteristics (RODRIGUES et al, 2009). Bergstein et al (2014) reported that although sperm motility and membrane integrity tests are important, these may not reveal relevant sperm lesions.…”

Section: Resultsmentioning

confidence: 99%

Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase

Triques

Schmidt

Oro

et al. 2016

SCA

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Because of their membrane constitution, sperm cells of male broiler breeders are prone to lipid peroxidation, which affects their fertilizing capacity. The aim of the present study was to evaluate the effects of antioxidant diet supplementation on the reproductive characteristics of 12 male broiler breeders (Cobb) older than 50 weeks. The roosters were randomly distributed in a completely randomized experimental design with two treatments (treatment 1: commercial feed; treatment 2: commercial feed supplemented with canthaxanthin, lycopene, and vitamin C) and 6 replicates. Semen samples were collected by abdominal massage, and then underwent evaluation of semen volume, sperm concentration, motility, vigor, and morphology. Morphometric analyses of testes, combs, and dewlaps were performed at 68 weeks. Testes samples were collected for morphometric analysis of seminiferous tubules and analysis of cellular proliferation in the germinal epithelium by using immunohistochemical staining with anti-proliferating cell nuclear antigen (anti-PCNA) antibodies. Statistical data analysis was performed using the SAS software (SAS, 2002). No significant effects of antioxidant supplementation were observed on semen characteristics (p > 0.05). A significant positive effect of the antioxidant blend was observed on the percentage of normal sperm, dewlap weight and width, and testes weight and length (p < 0.05). PCNA-positive cell counts and morphometry of the seminiferous tubules were not affected by the treatment. Dietary antioxidant supplementation may represent a nutritional tool to increase fertility in male broiler breeder flocks during the post-peak production phase. Key words: Canthaxanthin. Roosters. Lycopene. Lipid peroxidation. Vitamin C. ResumoOs espermatozoides de machos reprodutores de frango de corte são células propensas a peroxidação lipídica, dada à constituição biológica de sua membrana, influenciando a capacidade fertilizante do macho. O objetivo deste trabalho foi avaliar o efeito da suplementação dietética de antioxidantes

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Section: Resultsmentioning

confidence: 99%

Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase

Triques

Schmidt

Oro

et al. 2016

SCA

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“…A study in roosters showed larger sperm head area in younger males due to increased morphological abnormalities [34]. In contrast, older boars showed spermatozoa with larger head area than their younger counterparts [35].…”

Section: Discussionmentioning

confidence: 99%

Sperm morphometry and chromatin condensation in Nelore bulls of different ages and their effects on IVF

et al. 2017

Self Cite

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The aim of this study was to evaluate the chromatin packing and sperm head morphometry of cryopreserved semen of Nelore bulls (Bos taurus indicus) of different ages. Furthermore, the influence of the degree of chromatin compaction on in vitro embryo production (IVP) was investigated. Forty bulls were divided into three groups: young (1.8-2 years), adult (3.5-7 years), and senile (8-14.3 years). The ejaculates were frozen according to standards established by the Artificial Insemination Center located in the Southeast of Brazil. Toluidine blue staining was used for simultaneous evaluation of the sperm chromatin and sperm head morphometry. Chromomycin A3 (CMA3) was applied to analyze sperm protamination and IVP for embryonic development. Spermatozoa of young bulls presented higher values for area (A, pixels), perimeter (P, pixels), and width (W, pixels) compared to adults and senile (young: A = 1848.5 ± 119.79, P = 10.23 ± 0.29, and W = 1.95 ± 0.1; adults: A = 1672.9 ± 104.46, P = 9.86 ± 0.33, and W = 1.81 ± 0.06; senile: A = 1723.1 ± 124.41, P = 9.97 ± 0.33, and W = 1.83 ± 0.09; P < 0.0001) and showed higher protamination deficiency when analyzed by CMA3 (young: 1.57 ± 0.76; adults: 1.09 ± 0.63, and senile: 0.90 ± 0.59; P < 0.05). Likewise, variables of sperm head size (A, P, and W) and protamination assessed by CMA3 showed negative correlation with age and positive correlation with ellipticity, evaluated by toluidine blue method (P < 0.05). Sperm head area was larger in spermatozoa presenting chromatin instabilities than spermatozoa without chromatin alteration (P < 0.0001). There was no difference in IVP when using semen with larger or smaller portions of spermatozoa with chromatin instabilities, indicating that the proportion of sperm with abnormal chromatin compaction (4%-16.15%) did not interfere with early embryonic development. From our results, it can be concluded that sperm of young Nelore bulls have larger heads compared to adults and senile due to reduced protamine content when evaluated by CMA3 and higher proportion of major sperm defects assessed by differential interference contrast microscopy.

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“…Among these tests, SCSA is the most used (Beletti, 2013), but its high cost lowers its practical applicability. The toluidine blue technique is easy and inexpensive (Beletti and Mello, 2004;Erenpreiss et al, 2004), and it has been used for chromatin stability evaluation of many species: humans (Erenpreiss et al, 2001;Beletti and Costa, 2003;Erenpreiss et al, 2004), rabbit (Beletti and Mello, 2004), bovine, caprine, ovine, canine (Beletti and Costa, 2003), fowl (Rodrigues et al, 2009), equine (Naves et al, 2004) and porcine (Arraztoa et al, 2016). The toluidine blue technique was chosen for the present study because spermatozoa head morphology, morphometry, and chromatin can all be evaluated concomitantly, as described by Beletti et al (2005).…”

Section: Discussionmentioning

confidence: 99%

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

Gaggini¹,

Rocha²,

Souza³

et al. 2017

Anim Reprod

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In boar studs, morphological analyses are used to evaluate sperm quality and thereby categorize ejaculates as either approved or rejected. Normally, morphological characteristics correlate with chromatin disorders, but studies to date have only considered the average of abnormalities; cells were not segregated as normal or abnormal. The aim of this study was to assess whether the presence of cytoplasmic droplets was associated with morphometric characteristics and chromatin instability of spermatozoa heads. Morphological analyses were performed on semen from 11 boars using phase contrast microscopy (200 cells per sample). Normal cells were differentiated from those with cytoplasmic droplets and both types were evaluated separately. Photomicrographs were acquired of normal spermatozoa (Group NOR, N = 1,207) as well as spermatozoa with proximal and distal cytoplasmic droplets (Group DROP, N = 725). Sperm-head morphometry and chromatin structure were evaluated using the toluidine blue technique. Spermatozoa heads in the DROP group were longer (8.37 ± 0.60 × 8.31 ± 0.53; P = 0.025), narrower (4.16 ± 0.21 × 4.19 ± 0.19; P = 0.03), and more symmetric on the sides (0.973 ± 0.012 × 0.971 ± 0.011; P = 0.007) than were spermatozoa heads of the NOR group. The DROP group also had a greater average ellipticity (0.335 ± 0.034 × 0.329 ± 0.031; P = 0.0004), a greater percentage of decondensed chromatin (2.71 ± 3.87 × 2.28 ± 1.38; P < 0.0008), and a greater chromatin heterogeneity (4.66 ± 1.40 × 4.40 ± 1.42; P < 0.0001). A greater frequency of semen collection results in a shorter period of cell maturation and this probably affected the degree of chromatin condensation and the cytoplasmic droplet migration, with concomitant effect on the head morphometry measurements. In conclusion, compared with normal spermatozoa, those with cytoplasmic droplets show altered morphometric characteristics, such as longer and narrower spermatozoa heads. They likewise have greater chromatin instability, resulting in a higher percentage of decondensed chromatin and more heterogeneous chromatin.

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Análise computacional da compactação da cromatina de espermatozoides de galo

Cited by 11 publications

References 13 publications

Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase

Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase

Sperm morphometry and chromatin condensation in Nelore bulls of different ages and their effects on IVF

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

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