Análise das relações taxonômicas e sistemáticas entre espécies de triatomíneos (Hemiptera, Reduviidae) de colônias mantidas pelo Serviço Especial de Saúde de Araraquara, inferida de seqüências do 16S rDNA mitocondrial

Triatoma vitticeps (Stal, 1859) (Hemiptera, Triatominae): A Chagas Disease Vector or a Complex of Vectors?

“…13 The specimens used in cytogenetic studies were from the same populations of the material used for the molecular analysis. [14][15][16] All specimens analyzed showed the same cytogenetic characteristics: karyotype 2n = 24 (20A + X 1 X 2 X 3 Y) and heterochromatin restricted to only one of the X sex chromosomes and in the Y sex chromosome (Figure 1). These results demonstrate that T. vitticeps does not present intraspecific chromosome variation as already observed for other triatomines.…”

“…17 On the other hand, the different mitochondrial genes analyzed demonstrated high intraspecific variation between the genetic distances of T. vitticeps from different states ranging from 2.3% to 7.5% (Tables 2-4). Pereira et al 18 consider that 19 Based on this, different authors evaluated the specific status of triatomines by means of genetic distance using mitochondrial genes: Alevi et al 20 through the 16S gene corroborates the specific status of Triatoma pintodiasi Jurberg et al (2013); Alevi et al 21 through the 16S gene also have proposed the synonimization between Nesotriatoma bruneri Usinger (1944) and Nesotriatoma flavida (Neiva, 1911); Ceretti-Junior et al 14 based on the 16S gene reported low intraspecific variation between genetic distances of triatomines (0-0.6%), except for relationships between Triatoma brasiliensis Neiva (1911) specimens (1.3%) and Triatoma sordida (St ål, 1859) specimens (1%) from different geographic populations (specimens that possibly suffered cryptic speciation) 22,23 ; Panzera et al 23 using the COI gene observed a genetic distance of 5.3% between T. sordida from Brazil and from Argentina and suggest that T. sordida from Argentina is possibly a new cryptic species of T. sordida; Abad-Franch et al 24 used the genetic distance of cyt b (8.7%) between Rhodnius robustus Larrousse (1927) and Rhodnius barretti to characterize that R. barretti represents a distinct entity within the "robustus lineage"; and Rua et al 25 suggested that populations of Triatoma sanguisuga (Leconte, 1855) are divided into two subspecies because they have genetic distances close to five for cyt b.…”

Characterization of the Buccula, Rostrum, Stridulatory Sulcus, Scutellum, and External Female Genitalia ofTriatoma carcavalloi(Jurberg, Rocha & Lent, 1998),Triatoma circummaculata(Stål, 1859), andTriatoma rubrovaria(Blanchard, 1843) (Hemiptera, Reduviidae, Triatominae)

“…In cytogenetic, molecular, and morphometric analyses of the head, including the antennas, thorax, and abdomen, it was demonstrated that it is possible to distinguish completely between T. maculata (Erichson, 1848) , T. pseudomaculata (Corrêa & Espínola, 1964), and T. arthurneivai (Lent & Martins, 1940), with the evolutionary relation of the first species, in relation to the last ones, being questioned [13–15]. Isoenzymatic and chromatic results corroborate each other and favor the hypothesis of a distinct and isolated population T. rubrovaria [16].…”

Mitochondrial Gene Confirms the Specific Status of Triatoma pintodiasi Jurberg, Cunha, and Rocha, 2013 (Hemiptera, Triatominae), an Endemic Species in Brazil

“…It is important to note the utility of the 16S gene as molecular marker among triatomine species and its importance in systematic and taxonomic issues. 12 Furthermore, the extremely low genetic distance observed in this mitochondrial gene led to the suggestion that Nesotriatoma flavida and Nesotriatoma bruneri are the same species. 13 Phylogenetic analyses have shown the T. rubrovaria subcomplex to be monophyletic 11 (T. pintodiasi has never been included in these analyses due to its recent description).…”