Análise morfométrica da parede intestinal e dinâmica de mucinas secretadas no jejuno de frangos suplementados com probiótico Bacillus subtilis cepa C3102

Rocha, Priscilla Maria Cavalcante; Barros, Maria Edna Gomes de; Evêncio-Neto, Joaquim

doi:10.1590/s0100-736x2016000400010

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…Regarding the goblet cell counting, other authors also report the increase of these cells in situations of exposure to various xenobiotics (FLEMING et al, 2010;OEHLERS et al, 2012;DOS SANTOS et al, 2016). These cells participate in the maintenance and development of the intestinal epithelium and secrete mucus, an essential substance for protection against infections, which prevents the contact of microorganisms with intestinal epithelial cells (ROCHA et al, 2016). Increase in mucus production may represent a defense response to exposure to high concentrations of MnCl 2 , as observed in the acute experiment.…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

Rodrigues

Souza

Silva

et al. 2018

CeN

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Manganese is an essential element for many physiological processes. However, in prolonged exposures or at high concentrations it may present toxicity to several organs, which has made it an object of study in pharmacological, behavioral, neurological and environmental research in different organisms. Thus, it was aimed to evaluate the damage caused by acute and chronic exposures to this element, through the histopathological analysis of the zebrafish (Danio rerio) intestine. Samples were embedded in paraffin for further processing in rotating microtome, and the slides were stained with Alcian Blue and hematoxylin for observation and analysis under light microscopy. The results demonstrated the potential of the metal to cause histological changes in this organ, such as fusion of villi (in 0.5 and 4.0 mg L-1), and to increase by more than 50% the number of goblet cells (in 8.0 and 16 mg L-1) in the region analyzed, mainly in the highest concentrations of Manganese Chloride and in chronic exposures, characterizing a possible inflammation of the intestinal epithelium. Thus, the histological analysis of intestines demonstrated to be a reliable alternative for the toxicological evaluation. It is important to monitor the concentrations of this and other metals in the water bodies, given their toxicity.

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Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

Rodrigues

Souza

Silva

et al. 2018

CeN

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“…Segundo Batista et al (2016) trabalhando com suplementação de probióticos na ração para a espécie Solea senegelensis, verificaram que no intestino um aumento na área da secção, do comprimento das vilosidades e da largura dos vilos, indicando um aumento da área superficial demonstrando uma melhora na capacidade de absorção dos nutrientes. Rocha et al (2016), explicam que o probiótico Bacillus subtilis como proteção de forma antimicrobiana para os vilos e superfícies absortivas contra microrganismos patógenos em peixes, além, de auxiliarem na digestão e na absorção intestinal.…”

Section: Níveis De Probióticos (G/100g)unclassified

Probióticos do gênero Bacillus em dietas para pós-larvas de tilápia do Nilo (Oreochromis niloticus)

Castro

Xavier

Silva

et al. 2021

RSD

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Com o objetivo de avaliar diferentes níveis de probiótico em dietas para pós-larvas de tilápia do Nilo, 1.680 pós-larvas de tilápia do Nilo (0,05±0,01g) foram distribuídos em 24 aquários de 110 L, em um delineamento inteiramente casualizado com seis tratamentos e quatro repetições, considerando como unidade experimental um aquário com 70 alevinos. Durante o experimento foram monitorados diariamente os parâmetros de qualidade de água utilizando sonda multiparâmetro portátil sendo avaliado a temperatura da água (26,9±0,5°C), oxigênio dissolvido (4,0±0,7mg/L), condutividade elétrica (170,1±46,6μS/cm) e pH (7,2±0,5). Foi formulada uma dieta basal (controle – 0,00) e realizado níveis de inclusão de probiótico do gênero Bacillus 4,0x 1012 UFC (0,02; 0,04; 0,06; 0,08; 0,10 g/100g) de ração. Ao final, foram avaliados os parâmetros de desempenho zootécnico e composição centesimal da carcaça, onde não foi observada diferenças (P<0,05) sobre os parâmetros avaliados. Foi realizada histologia do intestino, e as medidas as altura e largura dos vilos e espessura da túnica, onde observou-se influência (P<0,05) sobre a largura dos vilose espessura da túnica, que apresentaram melhores respostas nos níveis de inclusões mais elevado de probióticos (0,08 e 0,10g). Apesar de, não haver influência positiva dos níveis de inclusão de probiótico do gênero Bacillus na dieta não influenciarem no desempenho zootécnico da tilápia do Nilo, recomenda-se a inclusão de 0,08g/kg de probiótico do gênero Bacillus por apresentar melhora na saúde intestinal das pós-larvas de tilápia do Nilo.

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“…Acid mucins are in uenced by the type of diet and form barriers against bacteria and agents that limit absorption (Rocha et al 2016).…”

Section: Intestinal Morphohistologymentioning

confidence: 99%

Dietary Lysine Requirements of Colossoma Macropomum (Cuvier, 1818) Based on Growth Performance, Hepatic and Intestinal Morphohistology and Plasma Biochemistry

ARdS

MdS

Wlp

et al. 2021

Preprint

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This study aimed to determine the dietary lysine requirements of juvenile Colossoma macropomum tambaqui based on growth performance. We also evaluated gut and hepatic histomorphometry as well as blood metabolites in accordance with the increased levels of dietary lysine. The juveniles (33.88 ± 2.47 g) were fed until apparent satiation with diets containing 6.60, 9.72, 12.84, 15.96, 19.08 and 22.20 g/kg of lysine. Fish were randomly distributed in groups of 10 fish per tank and assays were performed in triplicate, during 90 days. Tambaqui fed with 15.96 g/kg dietary lysine showed higher final weight (p = 0.001) and optimized feed conversion ratio (p = 0.001). Morphohistological modifications were present in livers of fish fed with low levels of lysine. In the proximal intestine, mucosa layer density was greater at the level of 15.96 g/kg (p = 0.001). In the middle intestine, height (p = 0.001) and perimeter (p = 0.001) of the villi were greater at low levels of lysine (respectively, 9.72 and 12.84 g/kg dietary lysine). Tambaqui fed with 15.96 g/kg of lysine achieved higher plasma protein concentrations (p = 0.01). Using the second-order polynomial regression analysis as support, and based on protein efficiency rate and body weight gain, dietary lysine requirement for juvenile tambaqui was calculated as 15.4–15.6 g/kg of diet (5.7–5.8% of dietary protein).

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Análise morfométrica da parede intestinal e dinâmica de mucinas secretadas no jejuno de frangos suplementados com probiótico Bacillus subtilis cepa C3102

Cited by 8 publications

References 15 publications

Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

Probióticos do gênero Bacillus em dietas para pós-larvas de tilápia do Nilo (Oreochromis niloticus)

Dietary Lysine Requirements of Colossoma Macropomum (Cuvier, 1818) Based on Growth Performance, Hepatic and Intestinal Morphohistology and Plasma Biochemistry

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