Analysis and construction of pathogenicity island regulatory pathways in Salmonella enterica serovar Typhi

Ong, Su Yean; Ng, Fui Ling; Badai, Siti Suriawati; Yuryev, Anton; Alam, Maqsudul

doi:10.2390/biecoll-jib-2010-145

Cited by 13 publications

(1 citation statement)

References 56 publications

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“…The proteome of S. Typhimurium cells is known to change dynamically throughout adhesion, colonization, invasion, intracellular survival, proliferation, and biofilm formation under the control of SPI gene expression [46,47]. To understand the effect of RNase G on T3SS expression, we examined the protein expression profiles in supernatants of the WT and Δrng strains by SDS-PAGE (S3 Fig) and the major protein bands between 30 to 90 kDa by tandem mass spectrometry.…”

Section: Effect Of Rnase G Levels On S Typhimurium Protein Profilesmentioning

confidence: 99%

Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression

et al. 2021

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Bacteria utilize endoribonuclease-mediated RNA processing and decay to rapidly adapt to environmental changes. Here, we report that the modulation of hns mRNA stability by the endoribonuclease RNase G plays a key role in Salmonella Typhimurium pathogenicity. We found that RNase G determines the half-life of hns mRNA by cleaving its 5′ untranslated region and that altering its cleavage sites by genome editing stabilizes hns mRNA, thus decreasing S. Typhimurium virulence in mice. Under anaerobic conditions, the FNR-mediated transcriptional repression of rnc encoding RNase III, which degrades rng mRNA, and simultaneous induction of rng transcription resulted in rapid hns mRNA degradation, leading to the derepression of genes involved in the Salmonella pathogenicity island 1 (SPI-1) type III secretion system (T3SS). Together, our findings show that RNase III and RNase G levels-mediated control of hns mRNA abundance acts as a regulatory pathway upstream of a complex feed-forward loop for SPI-1 expression.

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Section: Effect Of Rnase G Levels On S Typhimurium Protein Profilesmentioning

confidence: 99%

Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression

et al. 2021

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Acyl homoserine lactone-based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions

et al. 2016

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Quorum sensing regulates a variety of phenotypes in bacteria including the production of virulence factors. Salmonella spp. have quorum sensing systems mediated by three autoinducers (AI-1, AI-2, and AI-3). The AI-1-mediated system is incomplete in that the bacterium relies on the synthesis of signaling molecules by other microorganisms. This study aimed to evaluate the influence of the AI-1 N-dodecanoyl-DL-homoserine lactone (C12-HSL) on the growth, motility, adhesion, and biofilm formation of Salmonella enterica serovar Enteritidis PT4 578 on a polystyrene surface. Experiments were conducted at 37 °C in anaerobic tryptone soy broth supplemented with C12-HSL and/or a mixture of four synthetic furanones, at the concentration of 50 nM each. The planktonic growth, adhesion, swarming, and twitching motility were not altered in the presence of C12-HSL and/or furanones under anaerobic conditions. However, C12-HSL induced biofilm formation after 36 h of cultivation as determined by quantification of biofilm formation, by enumeration of adhered cells to polystyrene coupons, and finally by imaging the presence of multilayered cells on an epifluorescence microscope. When furanones were present in the medium, an antagonistic effect against C12-HSL on the biofilm development was observed. The results demonstrate an induction of biofilm formation in Salmonella Enteritidis by AI-1 under anaerobic conditions. Considering that Salmonella does not produce AI-1 but respond to it, C12-HSL synthesized by other bacterial species could trigger biofilm formation by this pathogen in conditions that are relevant for its pathogenesis.

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Furanone and phytol influence metabolic phenotypes regulated by acyl-homoserine lactone in Salmonella

et al. 2022

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Analysis and construction of pathogenicity island regulatory pathways in Salmonella enterica serovar Typhi

Cited by 13 publications

References 56 publications

Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression

Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression

Acyl homoserine lactone-based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions

Furanone and phytol influence metabolic phenotypes regulated by acyl-homoserine lactone in Salmonella

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