Analysis and in situ characterization of activated sludge communities capable of benzotriazole biodegradation

Herzog, Bastian; Huber, Birgit; Lemmer, Hilde; Horn, Harald; Müller, Elisabeth

doi:10.1186/2190-4715-25-31

Cited by 11 publications

(8 citation statements)

References 45 publications

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“…Moreover, a relationship between the abundance of β- Proteobacteria and the degree of pollution has been previously hypothesized (Brümmer et al, 2003). Indeed, an association between the β- Proteobacteria and benzotriazole biodegradation has been reported in activated sludge (Herzog et al, 2013). Furthermore, several species of β- Proteobacteria able to degrade 2,4-D, MCPA, metolachlor, terbuthylazine, other pesticides (Barra Caracciolo et al, 2010; Liu et al, 2011) and also PAHs (Ghosal et al, 2016; Singleton et al, 2009) have been found.…”

Section: Discussionmentioning

confidence: 99%

Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river

Saccà

Ferrero²,

Loos

et al. 2019

Science of The Total Environment

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The Water Framework Directive (WFD) regulates freshwater and coastal water quality assessment in Europe. Chemical and ecological water quality status is based on measurements of chemical pollutants in water and biota together with other indicators such as temperature, nutrients, species compositions (phytoplankton, microalgae, benthos and fish) and hydromorphological conditions. However, in the current strategy a link between the chemical and the ecological status is missing. In the present WFD, no microbiological indicators are foreseen for integrating the different anthropogenic pressures, including mixtures of chemicals, nutrients and temperature changes, to provide a holistic view of the freshwater ecosystem water quality. The main aim of this work was to evaluate if natural microbial populations can be valuable indicators of multiple stressors (e.g. chemical pollutants, temperature, nutrients etc.) to guide preventive and remediation actions by water authorities. A preliminary survey was conducted to identify four sites reflecting a contamination gradient from the source to the mouth of a river suitable to the objectives of the European Marie Curie project, MicroCoKit. The River Tiber (Italy) was selected as a pilot case study to investigate the correlation between bacteria taxa and the chemical status of the river. The main physicochemical parameters, inorganic elements, organic pollutants and natural microbial community composition were assessed at four selected sites corresponding to pristine, agricultural, industrial and urban areas for three consecutive years. The overall chemical results indicated a correspondence between different groups of contaminants and the main contamination sources at the selected sampling points. Phylogenetic analysis of the microbial community analyzed by Fluorescence In Situ Hybridization method (FISH) revealed differences among the four sampling sites which could reflect an adaptive bacterial response to the different anthropogenic pressures.

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Section: Discussionmentioning

confidence: 99%

Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river

Saccà

Ferrero²,

Loos

et al. 2019

Science of The Total Environment

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“…Of note, ASVs identified as Pseudomonas veronii , a taxon implicated in biotransformation of other aromatic compounds (e.g., Nam et al., ) made up 18% of sequences from MT, the source that resulted in the most BZT biotransformation (though nonsignificantly so) and the most urban site. It is possible that urban sites may select duckweed genotypes or favor microbes with increased BZT biotransformation, and indeed, others have found that microorganisms pre‐exposed to BZT had higher growth and biotransformation rates (Herzog et al., ; Kowalska and Felis, ).…”

Section: Discussionmentioning

confidence: 99%

“…We thus hypothesized (1) that NaCl would magnify negative effects of BZT on duckweeds and microbes. Some microbes and plants cause biotransformation of BZT (Herzog et al, 2013;Kowalska and Felis, 2015;LeFevre et al, 2015), raising the possibility that duckweeds and microbes could potentially be used for bioremediation of this emerging contaminant. Further, microbes can reduce plant salinity stress in several systems (Mayak et al, 2004;Bal et al, 2013;Rho et al, 2018).…”

mentioning

confidence: 99%

Resilience to multiple stressors in an aquatic plant and its microbiome

O'Brien

Luo

et al. 2019

American J of Botany

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Premise Outcomes of species interactions, especially mutualisms, are notoriously dependent on environmental context, and environments are changing rapidly. Studies have investigated how mutualisms respond to or ameliorate anthropogenic environmental changes, but most have focused on nutrient pollution or climate change and tested stressors one at a time. Relatively little is known about how mutualisms may be altered by or buffer the effects of multiple chemical contaminants, which differ fundamentally from nutrient or climate stressors and are especially widespread in aquatic habitats. Methods We investigated the impacts of two contaminants on interactions between the duckweed Lemna minor and its microbiome. Sodium chloride (salt) and benzotriazole (a corrosion inhibitor) often co‐occur in runoff to water bodies where duckweeds reside. We tested three L. minor genotypes with and without the culturable portion of their microbiome across field‐realistic gradients of salt (3 levels) and benzotriazole (4 levels) in a fully factorial experiment (24 treatments, tested on each genotype) and measured plant and microbial growth. Results Stressors had conditional effects. Salt decreased both plant and microbial growth and decreased plant survival more as benzotriazole concentrations increased. In contrast, benzotriazole did not affect microbial abundance and even benefited plants when salt and microbes were absent, perhaps due to biotransformation into growth‐promoting compounds. Microbes did not ameliorate duckweed stressors; microbial inoculation increased plant growth, but not at high salt concentrations. Conclusions Our results suggest that multiple stressors matter when predicting responses of mutualisms to global change and that beneficial microbes may not always buffer hosts against stress.

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“…Former studies already proved that wastewater treatment plants (WWTP), which receive domestic and industrial wastewater, constitute one major point source for these compounds to be released into the aquatic environment [ 6 , 12 - 15 ]. Therefore biodegradation studies, performed under specific laboratory conditions to exclude abiotic processes, are implicitly required to gain information about the biological removal potential of activated sludge communities as they are one way to reduce the input of these compounds into aquatic environmental systems [ 16 - 20 ]. Laboratory experiments already proved a completely different removal behavior of benzotriazole, 4- and 5-tolyltriazole [ 11 , 21 ] but biodegradation conditions remain rather unclear.…”

Section: Introductionmentioning

confidence: 99%

Screening and monitoring microbial xenobiotics’ biodegradation by rapid, inexpensive and easy to perform microplate UV-absorbance measurements

et al. 2014

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BackgroundEvaluation of xenobiotics biodegradation potential, shown here for benzotriazoles (corrosion inhibitors) and sulfamethoxazole (sulfonamide antibiotic) by microbial communities and/or pure cultures normally requires time intensive and money consuming LC/GC methods that are, in case of laboratory setups, not always needed.ResultsThe usage of high concentrations to apply a high selective pressure on the microbial communities/pure cultures in laboratory setups, a simple UV-absorbance measurement (UV-AM) was developed and validated for screening a large number of setups, requiring almost no preparation and significantly less time and money compared to LC/GC methods. This rapid and easy to use method was evaluated by comparing its measured values to LC-UV and GC-MS/MS results. Furthermore, its application for monitoring and screening unknown activated sludge communities (ASC) and mixed pure cultures has been tested and approved to detect biodegradation of benzotriazole (BTri), 4- and 5-tolyltriazole (4-TTri, 5-TTri) as well as SMX.ConclusionsIn laboratory setups, xenobiotics concentrations above 1.0 mg L-1 without any enrichment or preparation could be detected after optimization of the method. As UV-AM does not require much preparatory work and can be conducted in 96 or even 384 well plate formats, the number of possible parallel setups and screening efficiency was significantly increased while analytic and laboratory costs were reduced to a minimum.

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Analysis and in situ characterization of activated sludge communities capable of benzotriazole biodegradation

Cited by 11 publications

References 45 publications

Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river

Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river

Resilience to multiple stressors in an aquatic plant and its microbiome

Screening and monitoring microbial xenobiotics’ biodegradation by rapid, inexpensive and easy to perform microplate UV-absorbance measurements

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