Analysis and Pharmacokinetics of Apomorphine in Rat Brain by Microdialysis Coupled with Microbore HPLC Electrochemical Detection

Tsai, Tung Hu; Chen, C. F.; Cheng, Fu Chou; Kuo, Kou‐Wha; Tsai, Tsung-Yu

doi:10.1080/10826079708010665

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…As we know, HPLC and GC coupled with various detectors are powerful techniques for the analysis of compounds from complex matrices because their high capacity of separation and detection. In the previous studies, APO was separated by HPLC and detected by UV, fluorimetric and electrochemical detections 11–22. Among these previous studies, the highest sensitivity was achieved by using electrochemical detection with a LOD of 0.08 ng/mL 16.…”

Section: Resultsmentioning

confidence: 99%

“…High‐performance liquid chromatography (HPLC) coupled with different detectors including fluorimetric,11–13 UV,14, 15 and electrochemical16–22 detectors has been extensively adopted for determining APO in biological fluids. However, these HPLC methods required relatively long chromatographic times (usually more than 10 min) to complete a whole analytical procedure; as a result, these methods could not satisfy the high throughout required in PK studies.…”

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confidence: 99%

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Development of a sensitive and rapid liquid chromatography/tandem mass spectrometry method for the determination of apomorphine in canine plasma

Yang

Zhu

Deng

et al. 2006

Rapid Comm Mass Spectrometry

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A sensitive, rapid and specific quantitative liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination of apomorphine (APO) in canine plasma. The analytes were prepared using one-step liquid-liquid extraction, and analyzed on a Waters Symmetry C(18) column interfaced with triple quadrupole tandem mass spectrometer. A mixture of methanol/0.1% formic acid in water (70: 30, v/v) was employed as the isocratic mobile phase. Positive electrospray ionization was utilized as the ionization source. The analyte and clenbuterol (internal standard) were both detected using multiple reaction monitoring (MRM) mode. The limit of detection (LOD) obtained was 0.03 ng/mL. The assay was linear over the concentration range of 0.1-100 ng/mL, and provided good precision (RSD) and good accuracy (RE). The analyte was stable by using antioxidants throughout the whole study. The experimental results show that LC/MS/MS is a rapid and sensitive method to analyze APO in plasma. Finally, the proposed method was successfully applied to a pharmacokinetic study of APO after intranasal administration of 0.5 mg apomorphine to 10 healthy beagle dogs.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Development of a sensitive and rapid liquid chromatography/tandem mass spectrometry method for the determination of apomorphine in canine plasma

Yang

Zhu

Deng

et al. 2006

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

Determination of apomorphine in canine plasma by liquid chromatography–electrospray ionization mass spectrometry and its application to a pharmacokinetic study

Yang

Cai

et al. 2006

J. Sep. Sci.

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An LC-ESI-MS method was developed and validated for the assay of apomorphine in canine plasma using one-step liquid-liquid extraction. The analytes were separated on a Phenomenex Gemini C18 (150 mm x 2.0 mm id 3 microm) column and determined by MS in the positive ion mode. The linear range was 0.4-40 ng/mL with an LOD of 0.2 ng/mL for apomorphine in plasma. The intraday and interday precision and accuracy of quality control samples were < 5.9% RSD and < 7.5% bias for apomorphine. Extraction recoveries were > 80%. The validated method was successfully applied to analyze canine plasma samples in a pharmacokinetic study of apomorphine in dogs and detailed pharmacokinetic parameters were calculated.

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A review of microdialysis sampling for pharmacokinetic applications

Davies

1999

Analytica Chimica Acta

126

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Analysis and Pharmacokinetics of Apomorphine in Rat Brain by Microdialysis Coupled with Microbore HPLC Electrochemical Detection

Cited by 3 publications

References 22 publications

Development of a sensitive and rapid liquid chromatography/tandem mass spectrometry method for the determination of apomorphine in canine plasma

Development of a sensitive and rapid liquid chromatography/tandem mass spectrometry method for the determination of apomorphine in canine plasma

Determination of apomorphine in canine plasma by liquid chromatography–electrospray ionization mass spectrometry and its application to a pharmacokinetic study

A review of microdialysis sampling for pharmacokinetic applications

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