Analysis and purification of phorbol esters using normal phase HPLC and photodiode-array detection

Dimitrijevic, Sasa; Humer, U.; Shehadeh, Mayadah B.; Ryves, W. J.; Hassan, N. M.; Evans, Fred J.

doi:10.1016/s0731-7085(96)01867-5

Cited by 13 publications

(4 citation statements)

References 9 publications

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“…These compounds have been isolated from plants belonging to the family Euphorbiaceae. [26][27][28] Phorbols and their different derivatives are reported to be tumor promoters. These compounds do not induce tumors but promote tumor growth following exposure to a subcarcinogenic dose of a carcinogen.…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

et al. 2016

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Synadenium grantii is frequently used for the treatment of various diseases such as allergies, gastric disorders, and especially cancer. The aim of this study was to evaluate the possible antiproliferative potential of the methanol extract, fractions, and pure compounds from the stems of S grantii Phytochemical analysis was carried out by conventional chromatographic techniques, and the antiproliferative activity was analyzed using the sulforhodamine B assay and an MTT-based assay. Nonpolar fraction and its subfractions from the stems of S grantii exhibited promising cytostatic effect against several human tumor cell lines (glioma, breast, kidney, and lung), with total grown inhibition values ranging from 0.37 to 2.9 μg/mL. One of the active principles of this plant was identified as a rare phorbol diterpene ester, denoted as 3,4,12,13-tetraacetylphorbol-20-phenylacetate. This compound demonstrated antiproliferative activity against glioma, kidney, lung, and triple-negative breast cancer cell lines. These results demonstrate that S grantii stems produce active principles with relevant antiproliferative potential.

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Section: Discussionmentioning

confidence: 99%

Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

et al. 2016

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“…These compounds manifest various adverse biological effects, including inflammation [48], along with symptoms such as dizziness, vomiting, and diarrhea [32]. Additionally, several authors have reported other effects such as cell proliferation, platelet aggregation, lymphocyte mitogenesis, and prostaglandin production [4,15,41,43,49].…”

Section: Phorbol Estersmentioning

confidence: 99%

Detoxification Methods of Jatropha curcas Seed Cake and Its Potential Utilization as Animal Feed

de Barros,

Ferreira,

Fraga

et al. 2024

Fermentation

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Jatropha seed cake (JSC) derived from Jatropha curcas seeds is a by-product of biodiesel production and, due to its high protein content, has been considered as a potential animal feed ingredient. However, the presence of toxic compounds such as phorbol esters and other anti-nutritional factors limits its use in animal feeding. Several detoxification approaches have been used to tackle these constraints and this review aims to summarize the recent advances in JSC treatment aiming to enhance its potential as an animal feedstuff. The review first provides an overview of the structure and composition of phorbol esters and other anti-nutritional compounds, discussing its toxic effects on different animal species. It then explores several detoxification methodologies giving special emphasis to its effects on the nutritional composition of JSC and on the use of the treated substrate as a feed ingredient in fish, poultry, pigs, and ruminants, highlighting their growth performance, nutrient utilization, and animal health issues. Overall, the review concludes that these treatments hold great potential for the detoxification and utilization of JSC as an animal feed ingredient. However, further research is needed to optimize the treatment conditions, evaluate the economic feasibility, and assess the long-term effects of treated JSC on animal health and product quality.

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“…The basic structure of the compounds is a tigliane, daphnane, ingenane, and lathyrane skeleton. The classification was based on their basic pattern containing tri and tetracyclic ring systems [41]. Tigliane is the basic skeleton of phorbol ester (PE).…”

Section: Human Toxicity and Case Reportsmentioning

confidence: 99%

Rational use of Jatropha curcas L. in food and medicine: from toxicity problems to safe applications

et al. 2012

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Chapter 1 12 soil erosion. J. curcas L.is also known as the source of biodiesel, because from the seed, oil can be isolated by direct compression. This oil is used as a biofuel, candle and soap production, lighting and lubricant. In Europe the deoiled seedcake is believed to be suitable as animal feedstock and biofertilizer. In some rural areas in Indonesia the latex was traditionally used for treating toothache. Since J. curcas L. is considered as a future source of biodiesel, many people in Indonesia plant it in a huge plantation. they think if they can produce high amounts of oil from J. curcas L., it will replace the petroleum usage. Although, J. curcas L.is known to have many other usages, but the farmer did not realize they thought this plant only produced oil without any beneficial usage. So, they will be loss. This situation leads to a new concept that J. curcas L. should not only be used as biodiesel source, but it should give additional values to a farmer who plants this crop. 1 Ricinus communis seed 45-50 Non edible 2 Jatrophacurcas kernel 40-60 Non edible 3 Arachis hypogea kernel 35-55 Edible 4 Ceiba pentandra kernel 24-40 Non edible 5 Hevea brasilensis kernel 40-50 Non edible 6 Cocos nucifera kernel 60-70 Edible 7 Moringa oleifera seed 30-49 Edible 8 Aleurites moluccana kernel 57-69 Non edible 9 Sleichera trijuga kernel 55-70 Non edible Azadirachta indica kernel 40-50 Non edible Adenanthera pavonina Kernel 14-28 Edible Elais guineensis Pulp + Kernel 46-54 Edible Theobroma cacao Seed 54-58 Edible Sterculia foetida kernel 45-55 Non edible Callophyllum inophyllum kernel 40-73 Non edible Shorea stenoptera kernel 45-70 Edible Sesamum orientale seed 45-55 Edible Croton tiglium kernel 50-60 Non edible Annona muricata kernel 20-30 Non edible Annona squamosal seed 15-20 Non edible Cinnamomum burmanni seed 30 Edible Oryza sativa Bran 20 Edible Zea mays Germ 33 Edible Chapter 1 14 The aim of this thesis is to give an overview of the additional values of Jatropha curcas L. plant by characterization of its natural products that can be used as a safe pharmaceutical product. In addition the detoxicification of the plantcake allowing it to be used for animal stock has been researched. This thesis is a part of larger project for valorization Jatropha curcas L. plantation, especially in Indonesia. Recent developments in the technology of detoxification process and application of this ethnomedicinal plant to new fields of experimental medicine are reviewed in chapter 2. In this chapter recent data on biological activities, concepts and strategies for turning a toxic plant into a valuable crop with high pharmaceutical value are also discussed. A group of toxic compounds, which are relevant to study in J. curcas, are phorbol esters (PEs) since they are known as tumour promoter. In analysing those phorbol esters, phorbolmyristic acetate is used as a standard. This compound has two isomers which are α and β. In chapter 3 the differences between both isomers are discussed using LC-UV and LC-MS. Selecting wrong standard can ...

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Analysis and purification of phorbol esters using normal phase HPLC and photodiode-array detection

Cited by 13 publications

References 9 publications

Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

Detoxification Methods of Jatropha curcas Seed Cake and Its Potential Utilization as Animal Feed

Rational use of Jatropha curcas L. in food and medicine: from toxicity problems to safe applications

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