Analysis and Quantification of Oxidized Low-Density Lipoprotein-Induced Lipid Droplets in Macrophages Through High-Content Screening: Application for Antiatherogenic Drugs Discovery

Adipocyte-based high throughput screening for anti-obesity drug discovery: Current status and future perspectives

“…In the quantification of intracellular LDs [55] , we are able to calculate the average number of LDs or the total amount of fluorescence in adipocytes. However, if the number of cells is too small or too high, the conclusion will be incorrect.…”

Cobalt Chloride Induces Macrophage Foam Cell Formation: A Chemical Hypoxia Model for Anti-Atherosclerotic Drug Screening

“…The murine macrophage cell line, RAW 264.7, was purchased from the American Type Culture Collection (Manassas, VA) and cultured as described in our earlier study. 13 RAW 264.7 macrophages were maintained in cell culture medium composed of Dulbecco's modified Eagle medium (Invitrogen Life Technologies, Carlsbad, CA), 10% fetal bovine serum (Invitrogen), 100 unit/mL of penicillin (Invitrogen), and 100 mg/mL of streptomycin (Invitrogen) at 37°C in a humidified incubator with 5% CO 2 .…”

Inhibition of Ca2+-calpain signaling is a new mechanism using Laminaria japonica polysaccharide to prevent macrophage foam cell formation and atherosclerosis

“…Once the imbalance between lipid uptake and clearance occurs, the abnormal accumulation of intracellular lipid droplets will trigger foam cell formation. 2,3 The formation of foam cells is a critical pathological basis of atherosclerosis, which seriously threatens the cardiovascular health of humans. 4 According to the report issued by the World Health Organization, the deaths of atherosclerosis related cardiovascular diseases reach 17.9 million worldwide each year.…”