Analysis antifungal compounds produced by endophytic bacteria from oil plant using Bioautography

Abstract

This study was undertaken to evaluate the antifungal activities of the bioactive compounds poduced by endophytic bacteria isolated from oil palm plant using TLC-Bioautography. Antifungal compounds used in this study have been extracted from potential endophytic bacteria of previous studies using ethyl acetate solvent. The antifungal compounds was tested for its activity against the pathogenic fungus Fusarium oxysporum f.sp. by using TLC-Bioautography method. The results showed that there are 3 spots on the TLC plate for samples of B11 antifungal compound with an incubation time of 54 hours and B11 with an incubation time of 24 hours. Observation of bioautographic antifungal activity test showed the presence of inhibition zones with the largest average percentage given by B11 with an incubation time of 24 hours is 11.59%. The inhibition zone provided B11 with an incubation time of 54 hours is only 11.08%.

Inoculation of Endophytic Bacteria for Increasing Plant Height and Number of Rice Roots (Oryza sativa)

“…This phase is caused by reduced sources of nutrients, the formation of inhibitory compounds, and unfavorable environmental factors (Purnama, 2023). Endophytic bacteria will experience a lysis phase or death phase after passing through the stationary phase (Candrawati et al, 2018).…”

“…Growth curves are needed to determine the optimum incubation time for endophytic bacteria isolated from banana plants. The growth curve in this study was created by counting the number of bacterial cells during incubation at specific time intervals (Candrawati et al, 2018). The calculation of the number of bacterial cells to create a growth curve begins with preculture, in which bacterial isolates from oblique agar are inoculated into NB with a volume of 6 mL and incubated for 8 hours at room temperature at a speed of 100 rpm.…”