Analysis by elisa and Western blotting of antibody reactivities in cattle infected with Mycobacterium paratuberculosis after absorption of serum with M phlei

Milner, Anthony; Lepper, A.W.D.; Symonds, W.N.; Grüner, Eva

doi:10.1016/s0034-5288(18)30675-1

Cited by 82 publications

(37 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Kit was standardized as per method of Milner 15 with some modifi cation 16 . Briefl y, 0.1μg and 4.0 μg per plate of MAP 'Bison type' antigen (Kit 1), and commercial bovine antigen (Kit 2) respectively, were taken in 10 ml of carbonate-bi carbonate buffer, (pH 9.6) and coated in duplicate wells of fl at bottom 96 well ELISA plate.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits

Singh¹,

Singh²,

Singh³

et al. 2007

Indian J Microbiol

View full text Add to dashboard Cite

Country lacks sensitive and indigenous diagnostic kits for the screening of goats and sheep against Johne's disease. Therefore an indigenous ELISA kit was developed using protoplasmic antigen from native Mycobacterium avium subspecies paratuberculosis 'Bison Type' strain of goat origin (Kit 1). In the present study, kit 1 and two commercial kits (kit 2 and 3) were evaluated with respect to 'Gold Standard' fecal culture in 71 animals (55 goats and 16 sheep). Kit 1 using indigenous antigen (protoplasmic antigen) was sensitive at very low concentration (0.1 μgm / well) as compared to purifi ed commercial protoplasmic antigen (4 μgm / well) used in kit 2, in the Type 1 reactors (strong positive as positive). Screening of 71 animals by fecal culture detected 38.0% animals (goats-40.0%, sheep-31.2%) as positive (clinical shedders of bacilli) from these farm animals. Of the farm animals located at Central Institute for Research on Goats, herds of goat were endemic whereas, sheep fl ocks were comparatively resistant to Johne's disease. The 29.5 and 61.9, 15.4 and 57.7 and 4.2 and 14.0% animals (goats and sheep) were in the category of sero-reactors type 1 and 2 of the ELISA kits 1, 2 and 3, respectively. In the type 1 sero-reactors, sensitivity and specifi city of kit 1, 2 and 3 was 53.7 and 86.0, 17.8 and 86.0 and 3.5 and 94.7%, respectively. Indigenous ELISA test (kit 1) was signifi cantly superior for the screening of goatherds and sheep fl ocks against JD as compared to commercial ELISA kits (Kit 2 and 3).

show abstract

Section: Methodsmentioning

confidence: 99%

Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits

Singh¹,

Singh²,

Singh³

et al. 2007

Indian J Microbiol

View full text Add to dashboard Cite

show abstract

“…The sensitivity of culture was expected to be influenced by culture method and laboratory ; however, quantitative information on culture sensitivity by laboratory/specific method is lacking and changes in culture sensitivity were therefore not accounted for in the analysis. If animals were given two or more infectious doses of MAP in a short period of time and faecal cultures were reported in months postexposure, we assumed that values of time since infection were reported as time since first dose [4,5,[26][27][28][29][30].…”

Section: Standardization Of Experimental Data Across Studiesmentioning

confidence: 99%

A meta-analysis of the effect of dose and age at exposure on shedding ofMycobacterium aviumsubspeciesparatuberculosis(MAP) in experimentally infected calves and cows

et al. 2011

View full text Add to dashboard Cite

SUMMARYA meta-analysis was performed using all published and one unpublished long-term infection-challenge experiments to quantify the age- and dose-dependence of early and late shedding of Mycobacterium avium subsp. paratuberculosis (MAP) in cattle. There were 194 animals from 17 studies that fulfilled the inclusion criteria, of which 173 received a known dose of MAP and 21 were exposed naturally. Results from parametric time-to-event models indicated that challenging older calves or using multiple-exposure experimental systems resulted in a smaller proportion and shorter duration of early shedding as well as slower transition to late shedding from latent compartments. Calves exposed naturally showed variable infection progression rates, not dissimilar to other infection routes. The log-normal distribution was most appropriate for modelling infection-progression events. The infection pattern revealed by the modelling allowed better understanding of low-grade endemicity of MAP in cattle, and the parameter estimates are the basis for future transmission dynamics modelling.

show abstract

“…Tests available for diagnosis of bovine paratuberculosis include conventional fecal culture (HEY), 12,15,30,33,34 radiometric fecal culture (RCM), 6 the complement fixation (CF) t e s t , 9 , 1 4 , 2 0 , 2 3 , 2 6 agar gel immunodiffusion (AGID) test, [25][26][27] the absorbed enzyme-linked immunosorbent assay (ELISA), 8,17,18,36,37 and DNA or RNA gene probes. 29,31 Different sensitivities and specificities have been reported for each test, and conflicting results have been found for the same test, creating confusion as to the actual diagnostic accuracy of each test and the best test or combination of tests to be used.…”

mentioning

confidence: 99%

A Repository of Specimens for Comparison of Diagnostic Testing Procedures for Bovine Paratuberculosis

Sockett

Carr

Richards³

et al. 1992

J VET Diagn Invest

View full text Add to dashboard Cite

Analysis by elisa and Western blotting of antibody reactivities in cattle infected with Mycobacterium paratuberculosis after absorption of serum with M phlei

Cited by 82 publications

References 12 publications

Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits

Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits

A meta-analysis of the effect of dose and age at exposure on shedding ofMycobacterium aviumsubspeciesparatuberculosis(MAP) in experimentally infected calves and cows

A Repository of Specimens for Comparison of Diagnostic Testing Procedures for Bovine Paratuberculosis

Contact Info

Product

Resources

About