Analysis of a Human cDNA Containing a Tissue-Specific Alternatively Spliced LIM Domain

Putilina, Tatiana; Jaworski, Cynthia; Gentleman, Susan; McDonald, Benjamin D.; Kadiri, Maleck; Wong, Paul

doi:10.1006/bbrc.1998.9656

Cited by 23 publications

(25 citation statements)

References 18 publications

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“…Previous studies using Northern blot analysis investigated LMO7 expression in human adult and fetal tissues. In adults, LMO7 expression was found in lung, skeletal muscle, kidney, brain, and pancreas (Kang et al, 2000;Rozenblum et al, 2002) and in human fetal tissues in heart, lung, brain, kidney, and liver (Putilina et al, 1998). Except for the kidney all expression domains were also found in this study.…”

Section: Behsupporting

confidence: 64%

“…The LIM-only protein 7 (LMO7; PCD-1) was first described as a partial human pancreatic cDNA that encodes a single LIM domain (Putilina et al, 1998). LMO7 is a member of the PDZ and LIM domain-containing protein family and comprises not only an Nterminal LIM domain but contains also a C-terminal Calponin Homology (CH) domain and a PDZ domain.…”

Section: Introductionmentioning

confidence: 99%

“…Three splice forms have been described for human LMO7 (Putilina et al, 1998) and additional splice forms termed LMO7a, LMO7b (Ooshio et al, 2004) and LMO7S were found in the rat (Nakamura et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Additional expression was described in brain and pancreas adult tissue (Kang et al, 2000). Expression in fetal tissue was found to be high in the lung and heart and very weak in brain, kidney, and liver (Putilina et al, 1998). Microarray and semiquantitative RT-PCR studies of mouse hematopoietic cell lines showed that Lmo7 mRNA was absent from neuroblasts and T-lymphocytes (Lindvall et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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The lim domain only protein 7 is important in zebrafish heart development

Ott

Akker

Sakalis

et al. 2008

Developmental Dynamics

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The LIM domain only protein 7 (LMO7), a member of the PDZ and LIM domain-containing protein family is a candidate gene with possible roles in embryonic development and breast cancer progression. LMO7 has been linked to actin cytoskeleton organization through nectin/afadin and to cell-cell adhesion by means of E-cadherin/catenin. In addition, LMO7 has been shown to regulate transcription of the nuclear membrane protein Emerin and other muscle relevant genes. In this study, we used in situ hybridization to investigate LMO7 expression during embryonic development in three widely used vertebrate model species: the zebrafish, the chicken and the mouse. Our temporal and spatial gene expression analysis revealed both common and distinct patterns between these species. In mouse and chicken embryos we found expression in the outflow tract, the inflow tract, the pro-epicardial organ and the second heart field, structures highly important in the developing heart. Furthermore, gene knockdown experiments in zebrafish embryos resulted in severe defects in heart development with effects on the conduction system and on heart localization. In summary, we present here the first developmental study of LMO7. We reveal the temporal and spatial expression patterns of this important gene during mouse, chicken and fish development and our findings suggest essential functions for LMO7 during vertebrate heart development. Developmental Dynamics 237:3940 -3952, 2008.

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Section: Behsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The lim domain only protein 7 is important in zebrafish heart development

Ott

Akker

Sakalis

et al. 2008

Developmental Dynamics

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show abstract

“…overexpressed in breast cancers (Putilina et al, 1998;Kurihara et al, 2002). Inositol 1, 4, 5-triphospate receptor type 3 (IP(3)R3) is frequently expressed in secretion cells and known to be related to the IP3 signalling pathway, Ca 2+ influx, enzyme secretion and cell motility (Broad et al, 2001;Lee and Laychock, 2001), but its role in peritoneal dissemination is still unclear.…”

Section: Genetics and Genomicsmentioning

confidence: 99%

Differential gene expression profiles of gastric cancer cells established from primary tumour and malignant ascites

et al. 2002

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Advanced gastric cancer is often accompanied by metastasis to the peritoneum, resulting in a high mortality rate. Mechanisms involved in gastric cancer metastasis have not been fully clarified because metastasis involves multiple steps and requires a combination of altered expressions of many different genes. Thus, independent analysis of any single gene would be insufficient to understand all of the aspects of gastric cancer peritoneal dissemination. In this study, we performed a global analysis of the differential gene expression of a gastric cancer cell line established from a primary main tumour (SNU-1) and of other cell lines established from the metastasis to the peritoneal cavity (SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB). The application of a high-density cDNA microarray method made it possible to analyse the expression of approximately 21 168 genes. Our examinations of SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB showed that 24 genes were upregulated and 17 genes down-regulated besides expression sequence tags. The analysis revealed the following altered expression such as: (a) up-regulation of CD44 (cell adhesion), keratins 7, 8, and 14 (epitherial marker), aldehyde dehydrogenase (drug metabolism), CD9 and IP3 receptor type3 (signal transduction); (b) down-regulation of IL2 receptor g, IL4-Stat (immune response), p27 (cell cycle) and integrin b4 (adhesion) in gastric cancer cells from malignant ascites. We then analysed eight gastric cancer cell lines with Northern blot and observed preferential up-regulation and down-regulation of these selected genes in cells prone to peritoneal dissemination. Reverse transcriptase -polymerase chain reaction confirmed that several genes selected by DNA microarray were also overexpressed in clinical samples of malignant ascites. It is therefore considered that these genes may be related to the peritoneal dissemination of gastric cancers. The results of this global gene expression analysis of gastric cancer cells with peritoneal dissemination, promise to provide a new insight into the study of human gastric cancer peritoneal dissemination.

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Diseases of the Nucleoskeleton

Holaska

2016

Comprehensive Physiology

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The nucleus is separated from the cytosol by the nuclear envelope, which is a double lipid bilayer composed of the outer nuclear membrane and the inner nuclear membrane. The intermediate filament proteins lamin A, lamin B, and lamin C form a network underlying the inner nuclear membrane. This proteinaceous network provides the nucleus with its strength, rigidity, and elasticity. Positioned within the inner nuclear membrane are more than 150 inner nuclear membrane proteins, many of which interact directly with lamins and require lamins for their inner nuclear membrane localization. Inner nuclear membrane proteins and the nuclear lamins define the nuclear lamina. These inner nuclear membrane proteins have tissue-specific expression and diverse functions including regulating cytoskeletal organization, nuclear architecture, cell cycle dynamics, and genomic organization. Loss or mutations in lamins and inner nuclear membrane proteins cause a wide spectrum of diseases. Here, I will review the functions of the well-studied nuclear lamina proteins and the diseases associated with loss or mutations in these proteins. © 2016 American Physiological Society.

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Analysis of a Human cDNA Containing a Tissue-Specific Alternatively Spliced LIM Domain

Cited by 23 publications

References 18 publications

The lim domain only protein 7 is important in zebrafish heart development

The lim domain only protein 7 is important in zebrafish heart development

Differential gene expression profiles of gastric cancer cells established from primary tumour and malignant ascites

Diseases of the Nucleoskeleton

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