Analysis of a novel hypomorphic mutation in Trithorax-like gene affecting Drosophila melanogaster oogenesis

GAGA protein is essential for male germ cell development in Drosophila

“…It has previously been shown that some Trl mutations lead to female sterility due to serious defects during oogenesis (Bhat et al ., ; Ogienko et al ., ). By using different combinations of Trl mutations in this study, we have determined that mutant males also exhibit a significant decline in fertility and mature sperm production.…”

“…It is known that when active, the GAGA transcription factor has an intra‐nuclear localization and colocalizes with chromatin (Bhat et al ., ; Ogienko et al ., ). In the wild type, intra‐nuclear localization of anti‐GAGA antibody coincided with the early stages of spermatogenesis, that is in 2‐, 4‐, and 8‐cell cysts during interphase between mitosis, and in early 16‐cell cysts at the beginning of the synthetic phase (Fig.…”

“…It is interesting to note that a similar pattern of GAGA localization was observed in the ovaries of Trl R85 / Trl 362 mutant females. GAGA was clearly not detected in the germ‐line cells and cystoblast nuclei in the germarium and egg chambers (Ogienko et al ., ).…”

GAGA protein is required for multiple aspects of Drosophila oogenesis and female fertility

“…In Drosophila , in addition to Hox genes, GAF regulates a wide‐range of genes that are important for the formation of various organs and tissues at all stages of ontogenesis. It has been shown that GAF is necessary for embryogenesis, oogenesis, spermatogenesis, and eye and wing development (Bayarmagnai, Nicolay, Islam, Lopez‐Bigas, & Frolov, ; Bhat et al, ; Dos‐Santos et al, ; Ogienko et al, ; Ogienko et al, ; Omelina, Pavlova, Ogienko, & Baricheva, ). GAF is also involved in processes responsible for cellular memory, migration, and programmed cell death (Cavalli & Paro, ; Dorogova, Fedorova, Bolobolova, Ogienko, & Baricheva, ; Dorogova, Khrushcheva, Fedorova, Ogienko, & Baricheva, ; Mishra, Chopra, Srinivasan, & Mishra, ; Ogienko et al, ; Poux, Horard, Sigrist, & Pirrotta, ).…”

“…Previously, we showed that our originally generated mutations in Trl gene resulted in female sterility and caused a number of disruptions during oogenesis, including an abnormal number of oocytes and nurse cells, defects in cytoplasmic actin filament formation leading to rapid transport disorder, and failure of migration in different types of somatic cells (Ogienko et al, , , ). The range of abnormalities in oogenesis that we described earlier has a negative effect on oocyte formation, leading to a decrease in female fertility.…”