Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

“…None of these PI positive bacteria were able to recover and grow on appropriate media after cell sorting, which agrees with others [22,43,44]. PI was therefore considered testimony of dead cells.…”

Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures

“…In both cases within 4 h of inoculation almost all cells were either only Chamsartra et al, 2005;Amanullah et al, 2002) and the dual staining combination with PI/bisoxanol is suitable for Gram -ve cells e.g. E. coli, Salmonella spp., Acinetobacter johnsonii (Boswell et al, 1998;Want et al, 2009.;Hewitt & Nebe-von-Caron 2001;Nebe-von-Caron et al, 2000;Boswell et al, 1998). This is likely to be species and process specific whilst the interpretation of such data can only be reliably …”

Flow cytometry analysis of germinating Bacillus spores, using membrane potential dye

“…Recently, new viability assays were developed for bacteria (Keer and Birch 2003). These tests were essentially based on respiratory activity (Bhupathiraju et al 1999;Rodriguez et al 1992), external membrane integrity (Nebe-von-Caron et al 2000), intracellular pH (Chitara et al 2000), and membrane potential (Deere et al 1995;Manson et al 1993Manson et al , 1994Manson et al , 1995. The major advantage of these new technologies is the rapidity of response and the multi-parametric nature of the collected information.…”