Analysis of c-kit protein expression in small-cell lung carcinoma and its implication for prognosis

Follicular Expression of c-Kit/SCF and Inhibin-α in Mouse Ovary During Development

“…As shown in the negative controls in Figure 1, there was no immunological staining ( Figure 1C, 1F, and 1I). IHC localization for c-kit was identified in small-cell lung carcinoma (SCLC) and has been used as a marker for SCLC (Sekido et al 1991;Tsuura et al 1994;Naeem et al 2002). SCF was expressed in some epithelial cells of fetal lung terminal bronchi.…”

Expression of the tyrosine kinase c‐kit is an independent prognostic factor in patients with small cell lung cancer

“…As described by Naeem et al 11 and Micke et al, 12 immunohistochemical staining was performed for standardized c-kit detection, using a primary antibody against c-kit that targets the COOHterminal domain of the intracellular part of the c-kit receptor (catalogue 4502; Dako, Hamburg, Germany). Briefly, fresh tumor tissue specimens were immediately formalin-fixed following bronchoscopy or surgery.…”

“…9 -12 The reported differences can be explained by the use of different criteria to rate tumors as c-kit ϩ or not. For example, Naem et al 11 found 53% of 30 SCLC tumors to be c-kit ϩ ; in their definition, only tumors with Ͼ50% c-kit-expressing cells were regarded as c-kit ϩ . In contrast, in the study of Micke et al, 12 a tumor was accepted as c-kit ϩ if Ͼ10% of all tumor cells had "strict, clear-cut positive expression" of c-kit.…”

“…In the literature, proportions between 27.9% 9 and 78% 10 of c-kit ϩ SCLC tumors are described. Further, c-kit has been associated with both unfavorable prognosis 11,12 and favorable prognosis 9 and has not been a predictor of survival at all. 13 In the present study, we examined the prognostic value of c-kit expression on survival in 203 patients with SCLC.…”

C-Kit (CD117) Expression in Patients with Small Cell Lung Cancer

“…Biyopsi örneklerinde yapılan çalışmalarda ise c-kit ekspresyon sıklığı %53,3 veya %37 olarak rapor edilmiştir (13,14). Bizim çalışmamızda ise KHAK'lı hastaların c-kit ekspresyonu parafin bloklarda %24,1 olarak bulunmuştur.…”