Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of Klebsiella pneumoniae

Abstract

Klebsiella pneumoniae (K. pneumoniae) is an opportunistic bacterium that has drawn attention due to its resistance to carbapenem antibiotics. The treatment of patients with severe infections has been challenging. Thus, silver nanoparticles (AgNPs) have been applied for their antimicrobial effects. This work aims to analyze the synergistic effect of the carbapenem antibiotic Imipenem with AgNPs against different susceptibility clinical profiles of K. pneumoniae. The silver nanoparticles were synthesized by bottom-up methodology and capped with alpha-lipoic acid. Susceptibility tests were performed using four K. pneumoniae strains with different susceptibility profiles to Imipenem. The strains were induced to form a biofilm for 48 h. Crystal violet and Resazurin assays were performed to determine biofilm formation and minimal inhibitory concentration, respectively. The reduction in Imipenem concentration with the association of nanoparticles was found in all strains studied in planktonic form, and the synergism between silver nanoparticles and Imipenem was demonstrated through the analysis of the fractional inhibitory concentration index. The viability percentage was reduced at rates ≥80% in the biofilm analysis, characterized by the minimal biofilm inhibitory concentration. The study’s proposed association resulted in inhibitory effects on different K. pneumoniae profiles, both in planktonic forms and biofilm, with peculiar behavior in the Imipenem-resistant profile.

RETRACTED: Eradication of Klebsiella pneumoniae pulmonary infection by silver Oxytetracycline Nano-structure

“…The MIC was decreased by the antibiotic nanoparticles combination; this value was more significant for nanoparticles carrying both ciprofloxacin and imipenem against Staphylococcus aureus (Shafiei et al 2020). The combination of AgNPs with Imipenem caused MIC assays of antibiotic concentrations to decrease roughly by a range of 16 to 256 fold (Fontoura et al 2023). The presence of surfactant vesicles might lower the MIC values of antibiotics more than free (Abo Kamer et al 2023).…”

Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis

“…Once the antibiotic has a specific target and the AgNPs work in several ways to cause the microorganism’s death, it is suggested that synergy occurs when these effects are summed up due to the functionalization of nanoparticles with vancomycin. Several studies reported that different combinations of these two types of components could exhibit synergistic antibacterial effects [ 33 , 39 – 41 ]. In the studies by Buszewski et al .…”

Eradication of Klebsiella pneumoniae pulmonary infection by silver oxytetracycline nano-structure

“…The MIC was decreased by the antibiotic nanoparticles combination; this value was more significant for nanoparticles carrying both ciprofloxacin and imipenem against Staphylococcus aureus (Shafiei et al 2020 ). The combination of AgNPs with Imipenem caused MIC assays of antibiotic concentrations to decrease roughly by a range of 16 to 256 fold (Fontoura et al 2023 ). The presence of surfactant vesicles might lower the MIC values of antibiotics more than free (Abo Kamer et al 2023 ).…”

“…It has been demonstrated that using Nano-carriers (as Nano-structure) for conventional antibiotics improves their antibacterial efficacy against drug resistant microorganisms (Ficai and Grumezescu 2017). Adhesion of nanostructures to microbial cell surfaces, causes numerous cell wall deformation at sites of adhesion (Luan et al 2018).…”

“…1a; Surfactants were used to create new kinds of improved highly ordered active Ag-OTC-Ns (Wei et al 2019). It has been demonstrated that using Nano carriers (Nano-structure) for improves conventional antibiotics efficacy against XDR microorganisms (Ficai and Grumezescu 2017). The ability of these nanostructures to modify their geometry, shell morphology, and shell material had attracted a lot of attention because of their unique properties and potential applications ranging from antimicrobial and anti-inflammatory efficacy to drug delivery.…”