Analysis of chitin synthase function in a plant parasitic nematode, Meloidogyne artiellia, using RNAi

Fanelli, Elena; Vito, M. Di; Jones, John T.; Giorgi, Carla De

doi:10.1016/j.gene.2004.11.045

Cited by 105 publications

(67 citation statements)

References 27 publications

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“…Attempts to develop similar gene disruption methods for plant-parasitic nematodes in order to assess the role of cellwall-degrading enzymes in parasitism have been fruitless. However, recent developments to knock down genes by RNA interference in other plant-parasitic nematodes currently are being adopted to M. incognita (Fanelli et al 2005;Rosso et al 2005;Urwin et al 2002), and these methods may provide information about the influences of endo-1,4-β-xylanases and other cell-wall-degrading enzymes on the host plant range of plantparasitic nematodes.…”

Section: Discussionmentioning

confidence: 99%

A Symbiont-Independent Endo-1,4-β-Xylanase from the Plant-Parasitic NematodeMeloidogyne incognita

Mitreva¹,

Roze²,

Overmars³

et al. 2006

MPMI

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Substituted xylan polymers constitute a major part of the hemicellulose fraction of plant cell walls, especially in monocotyledons. Endo-1,4-β-xylanases (EC 3.2.1.8) are capable of hydrolyzing substituted xylan polymers into fragments of random size. Many herbivorous animals have evolved intimate relationships with endosymbionts to exploit their enzyme complexes for the degradation of xylan. Here, we report the first finding of a functional endo-1,4-β-xylanase gene from an animal. The gene (Mi-xyl1) was found in the obligate plant-parasitic root-knot nematode Meloidogyne incognita, and encodes a protein that is classified as a member of glycosyl hydrolase family 5. The expression of Mi-xyl1 is localized in the subventral esophageal gland cells of the nematode. Previous studies have shown that M. incognita has the ability to degrade cellulose and pectic polysaccharides in plant cell walls independent of endosymbionts. Including our current data on Mi-xyl1, we show that the endogenous enzyme complex in root-knot nematode secretions targets essentially all major cell wall carbohydrates to facilitate a stealthy intercellular migration in the host plant.

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Section: Discussionmentioning

confidence: 99%

A Symbiont-Independent Endo-1,4-β-Xylanase from the Plant-Parasitic NematodeMeloidogyne incognita

Mitreva¹,

Roze²,

Overmars³

et al. 2006

MPMI

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“…Since then, RNAi has been used to investigate pathogenicity-related genes in plant parasitic cyst-and root-knot nematodes (Rosso et al, 1999;Urwin et al, 2002;Fanelli et al, 2005;Chen et al, 2005;Huang et al, 2006). Various methods have been developed for uptake of double-stranded RNA (dsRNA), including microinjection with dsRNA, soaking worms in a dsRNA solution, and feeding worm bacteria that express dsRNA; these are the three most frequent RNAi methods (Park et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of the cellulose gene of the pine wood nematode, Bursaphelenchus xylophilus, using RNA interference

Lu²,

Jin

et al. 2011

Genet. Mol. Res.

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“…elegans shares common ancestry with plantparasitic nematodes (Riddle, 1997) and it has been demonstrated that much like C. elegans, Meloidogyne artiellia, Heterodera glycines and Globodera pallida are sensitive to RNAi (Fanelli et al, 2005;Urwin et al, 2002). This suggests that RNAi mechanisms are conserved among nematode species.…”

Section: Discussionmentioning

confidence: 99%

Effects of ced-9 dsRNA on <i>Caenorhabditis elegans</i> and <i>Meloidogyne incognita</i>

Gaeta¹

2011

American Journal of Agricultural and Biological Sciences

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Problem Statement: In metazoans Programmed Cell Death (PCD) is essential for proper development. Suppression of PCD is needed to guarantee cell survival and in the nematode Caenorhabditis elegans the regulation of PCD is accomplished by the function of the ced-9 gene. Approach: In this work the use of double stranded RNA (dsRNA) to knock-down ced-9 gene function was tested as means to induce PCD. Results: Our results indicate that dsRNA targeting the cell death protection gene ced-9 is effective at decreasing the fecundity of C. elegans by up to 21%. The decreased fecundity correlated with an increased presence of cell corpses in developing embryos. Endogenous ced-9 transcript levels were reduced in progeny of ced-3 mutant nematodes fed bacteria expressing ced-9 dsRNA. These data suggest that nematode fecundity can be reduced by ingestion and exposure to dsRNAs targeting regulation of the cell death pathway. In an attempt to determine if plant parasitic nematodes are susceptible to the targeting of the PCD regulatory pathway we exposed Meloidogyne incognita, a plant parasitic nematode, to ced-9 dsRNA; here we show that this exposure results in decreased gall formation in the tobacco plants. Conclusion/Recommendations: Our results provide the first steps toward using RNAi technologies to attempt nematode control by targeting cell death pathways. Ongoing research with transgenic plants designed to express dsRNA for ced-9-like sequences will further test the feasibility of generating plants with RNAi-based resistance to parasitic nematodes.

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Analysis of chitin synthase function in a plant parasitic nematode, Meloidogyne artiellia, using RNAi

Cited by 105 publications

References 27 publications

A Symbiont-Independent Endo-1,4-β-Xylanase from the Plant-Parasitic NematodeMeloidogyne incognita

A Symbiont-Independent Endo-1,4-β-Xylanase from the Plant-Parasitic NematodeMeloidogyne incognita

Functional analysis of the cellulose gene of the pine wood nematode, Bursaphelenchus xylophilus, using RNA interference

Effects of ced-9 dsRNA on <i>Caenorhabditis elegans</i> and <i>Meloidogyne incognita</i>

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