Analysis of cotton (Gossypium hirsutum) root proteomes during a compatible interaction with the black root rot fungus Thielaviopsis basicola

Abstract

A proteomic approach was used to uncover the inducible molecular defense mechanism of cotton root occurring during the compatible interaction with Thielaviopsis basicola. Microscopic observation of cotton root inoculated with a suspension of conidia showed that this necrotrophic hemibiotroph fungus interacts with the plant and completes its life cycle in our experimental system. 2-DE analysis of root extracts taken after 1, 3, 5, and 7 days postinoculation and cluster analysis of the protein expression levels showed four major profiles (constant, upregulated, one slightly downregulated, and one dramatically downregulated). Spots significantly (p<0.05) upregulated were analyzed by LC-MS/MS and identified using MASCOT MS/MS ion search software and associated databases. These proteins included defense and stress related proteins, such as pathogenesis-related proteins and proteins likely to be involved in the oxidative burst, sugar, and nitrogen metabolism as well as amino acid and isoprenoid synthesis. While many of the identified proteins are common components of the defense response of most plants, a proteasome subunit and a protein reported to be induced only in cotton root following Meloidogyne incognita infection were also identified.

Scoping the potential uses of beneficial microorganisms for increasing productivity in cotton cropping systems

“…Molecular techniques, such as proteomics and transcriptomics, add a new dimension to the understanding of the overall responses of plants and pathogens during disease cycles (Nittler et al, 2005;Coumans et al, 2009Coumans et al, , 2010Coumans et al, , 2011. Such information can be useful in the development of disease control measures, including biocontrol.…”

Metarhizium anisopliae host–pathogen interaction: differential immunoproteomics reveals proteins involved in the infection process of arthropods

“…Recent works have used proteomics to discover novel compounds for application in biological control (Suarez et al 2005;Wang et al 2005a, b;Coumans et al 2009). Also, immunoproteomic was applied to identify immunogenic proteins in vaccine development (Wu et al 2008;Barbey et al 2009), clinical biomarkers (Pedersen et al 2005) and comparative analysis (Shin et al 2007).…”

Proteomics-based analysis reveals that Verticillium dahliae toxin induces cell death by modifying the synthesis of host proteins

“…2) showed a temporary upregulation; their expression increased by 24 h and subsequently decreased by 72 h after inoculation. Temporarily upregulated proteins that have a potential role in modulating plant responses to ROS were also reported for cotton infected by Thielaviopsis basicola (Coumans et al 2009). Spot b11 (Fig.…”