Analysis of decellularized mouse liver fragment and its recellularization with human endometrial mesenchymal cells as a candidate for clinical usage

Evaluation of Vascular Endothelial Growth Factor Gene Expression in Recellularized Liver Tissue by Mouse Embryo Fibroblast

“…Decellularization of mouse liver tissue fragments (n = 12) was achieved using a combination of Triton X-100 and 1% SDS as described previously [ 11 ] . Briefly, fragments of liver tissue were placed in Triton X-100 on a stirrer at 50 rpm for 24 hours, after washing with PBS.…”

“…Decellularized scaffolds can be manufactured through eliminating cellular components from the native tissue using detergent perfusion and enzyme solutions and/or physical methods [ 1 , 2 ] . Triton X-100 and SDS are common detergents that are widely used by investigators for the removal of cellular materials [ 8 - 11 ] . While the cells are destroyed by these detergents, the structure of decellularized liver tissue is well preserved.…”

“…In DLS, critical molecules that involve in cell attachment, migration, and proliferation, such as glycoproteins, proteoglycans, and polysaccharides, are maintained [ 12 ] . Our recent study has demonstrated that the application of Triton X-100 in combination with 0.1% SDS for 24 hours for decellularization of mouse liver fragments, results in favorable preservation of tissue morphology and the ECM molecules similar to the native control [ 11 ] .…”

“…For the mentioned purposes, the decellularized liver tissues are repopulated by several methods and using a variety of somatic and stem cells [ 10 , 13 - 15 , 18 , 19 ] . The main sources of cells applied for repopulating the decellularized liver tissue are primary hepatocytes derived from embryonic or adult tissues and mesenchymal stem cells obtained from bone marrow or adipose tissues [ 10 , 11 - 15 , 18 , 19 ] . Our previous study demonstrated that the mouse DLS facilitated the adhesion and penetration of human endometrial mesenchymal cells [ 11 ] .…”

“…The main sources of cells applied for repopulating the decellularized liver tissue are primary hepatocytes derived from embryonic or adult tissues and mesenchymal stem cells obtained from bone marrow or adipose tissues [ 10 , 11 - 15 , 18 , 19 ] . Our previous study demonstrated that the mouse DLS facilitated the adhesion and penetration of human endometrial mesenchymal cells [ 11 ] . Angiogenesis plays a crucial role in regeneration of damaged tissues, women's menstruation, pregnancy, and wound healing, and insufficient angiogenesis results in impaired healing of damaged tissues [ 20 ] .…”