Analysis of differential expression profile of miRNA in peripheral blood of patients with lung cancer

Abstract

Purpose To identify potential molecular targets for lung cancer intervention and diagnosis, we analyzed the differential miRNA expression of peripheral blood between lung cancer patients and healthy controls. Methods Three pairs of cases’ and controls’ peripheral blood samples were evaluated for miRNA expression by microarray. 12 miRNAs were selected for RT‐PCR validation and target genes prediction. In addition, 4 miRNAs were selected for future validation by RT‐PCR in a large sample of 145 cases and 55 frequency‐matched healthy controls. Results A total of 338 differentially expressed miRNAs were screened and identified by microarray. According to the fold changes, the top ten upregulated miRNAs were hsa‐miR‐124‐3p, hsa‐miR‐379‐5p, hsa‐miR‐3655, hsa‐miR‐450b‐5p, hsa‐miR‐29a‐5p, hsa‐miR‐200a‐3p, hsa‐miR‐542‐3p, hsa‐miR‐138‐5p, hsa‐miR‐219a‐2‐3p, and hsa‐miR‐4701‐3p, and the top ten downregulated miRNAs were hsa‐miR‐34c‐5p, hsa‐miR‐135a‐5p, hsa‐miR‐132‐3p, hsa‐miR‐3178, hsa‐miR‐4449, hsa‐miR‐4999‐3p, hsa‐miR‐1246, hsa‐miR‐4424, hsa‐miR‐1252‐5p, and hsa‐miR‐24‐2‐5p. RT‐PCR verification of the 12 miRNAs revealed that 5 of 8 upregulated miRNAs, 2 of 4 downregulated miRNAs showed a significant difference between the cases and controls (P < .05). A large number of target genes and their functional set showed overlapping among the 453 predicted target genes of the 12 miRNAs (P < .01). RT‐PCR in the large sample confirmed the significant differential expression level of hsa‐miR‐29a‐5p, hsa‐miR‐135a‐5p, hsa‐miR‐542‐3p, and hsa‐miR‐4491 between cases and controls (P < .05), and three of these microRNA, except hsa‐miR‐29a‐5p, were significant after Bonferroni correction for adjustment of multiple comparisons. Conclusion There was a significant difference in miRNAs expression in the peripheral blood between lung cancer patients and healthy controls, and 4 miRNAs were validated by a large‐size sample.

High-Throughput MicroRNA Profiling of Vitreoretinal Lymphoma: Vitreous and Serum MicroRNA Profiles Distinct from Uveitis

“…High expression of this miRNA in vitreous and serum could provide insight into its role, although further study is needed to analyze the its function. Since only very few miRNAs and their target genes have been identified, and the functions of most miRNAs remain unclear [73], such insight into the interplay of miRNAs in VRL would help further steer rational diagnostic and therapeutic strategies and ultimately improve patient outcomes.…”

Non-coding RNAs in cancer: platforms and strategies for investigating the genomic “dark matter”

“…This method is often used to validate data obtained from microarray, as reported i.e. by He et al, which analyzed the differential expression profile of miRNAs in peripheral blood of lung cancer patients [66]. RT-qPCR technique include both TaqMan and SYBR green assays.…”

Analysis of the differential expression profile of miRNAs in myocardial tissues of rats with burn injury

“…MicroRNAs (miRNAs) are a type of endogenous noncoding small RNAs with a length of about 19-23 nucleotides [8]. To regulate the expression of their target genes at the post-transcriptional level, mature miRNAs mainly suppress the translation of their target mRNA by complementary base pairing [9]. miRNAs present in the fluids including urine, serum, plasma, tear and amniotic fluid, and they can resistant to harsh conditions [10].…”

“…miRNA also present in exosomes which can be taken up by neighboring or distant cells and subsequently modulate recipient cells [11]. So miRNAs could be a marker of intervention and diagnosis, such as hsa-miR-135a-5p, hsa-miR -542-3, and hsa-miR-4491 maybe the intervention and diagnosis marker of lung cancer via blood test [9]. It has also shown that miRNA can bind to hundreds of target mRNAs and play regulatory roles in almost all mammalian pathological and physiological activities.…”

“…It has also shown that miRNA can bind to hundreds of target mRNAs and play regulatory roles in almost all mammalian pathological and physiological activities. Furthermore, miRNAs are closely associated with the occurrence and development of several diseases [9]. Some miRNAs related to burn injuries, such as miR-21 which can be affected by heat-induced damage acts in response to the thermal stress by binding to Bcl-2 [12].…”