Analysis of DNA methylation patterns associated with drought stress response in faba bean ( Vicia faba L.) using methylation-sensitive amplification polymorphism (MSAP)

Abstract

Analysis of DNA methylation patterns associated with drought stress response in faba bean (Vicia faba L.) using methylation-sensitive amplification polymorphism (MSAP).Environmental and Experimental Botany http://dx.

The contrasting response to drought and waterlogging is underpinned by divergent DNA methylation programs associated with gene expression in sesame

“…Similar conclusions were drawn from the epigenetic responses of clover and hemp which decreased the methylation level under chromium stress but an active de novo synthesis of methylated cytosine was found to positively correlate with the intensity of the stress in the rape genome (Aina et al, 2004;Labra et al, 2004). Nonetheless, we deduced that hypermethylation under drought stress is a tolerance strategy in sesame and it would be valuable to compare in a future experiment the epigenetic alterations of (1) several sesame genotypes with contrasting levels of drought tolerance to understand the intra-species variation (Abid et al, 2017), (2) various plant species exhibiting contrasting responses to drought to uncover the inter-species variation (Aina et al, 2004).…”

“…Similarly, a quantification of the genome wide cytosine methylation polymorphism using the MSAP analysis showed a predominant hypomethylation in a tolerant rice accession under drought stress (Gayacharan and Joel, 2013). Furthermore, Abid et al (2017) recently noticed that drought stress reduces the methylation level in two faba bean genotypes, irrespective to their tolerance level. These contrasting reports imply that different methylation mechanisms for drought tolerance exist in plant species.…”

“…Under drought treatment, a decrease of DNA methylation was observed in leaf tissues of two faba bean genotypes (Abid et al, 2017). Moreover, Bednarek et al (2017) observed an elevated demethylation in both non-tolerant and tolerant plants, with de novo methylation occurring less frequently than demethylation under Aluminum stress in triticale lines.…”

“…The methylation-sensitive amplified polymorphism (MSAP) technique developed by Reyna-López et al (1997) is an adaptation of amplified fragment length polymorphism analysis and has proven to be a powerful tool for analyzing DNA methylation. The MSAP technique has been applied to study CpG methylation in the genome of plants, somaclonal epigenetic variation, cytosine methylation during various developmental stages and resistance to biotic and abiotic stresses (Ashikawa, 2001;Matthes et al, 2001;Portis et al, 2004;Sha et al, 2005, Ruiz-Garcia et al, 2005Tang et al, 2014;Zhang et al, 2015;Bednarek et al, 2017;Abid et al, 2017). Different approaches have been proposed to interpret MSAP outputs (Schulz et al, 2013;Fulneček and Kovařik, 2014), but none takes into account the multiple events reflecting various methylation patterns that must take place simultaneously to explain the differences in individual digestion patterns between control and stressed materials.…”

Response of DREB transcription factor to drought stress based on DNA methylation in wheat

“…Many studies have found that degree and state of DNA methylation in plant would change under drought stress, low temperature, high salt and other conditions [32,33], especially change of methylation state in the promoter of gene [34]. Under drought stress, methylation level altered in the promoter of DREB2, DREB6 and Wdreb2, compared with the control, methylation level in DREB2 and DREB6 promoter decreased as stressed for 2h, then increased along with the increase of stress time, which was opposite in Wdreb2 promoter.…”