Analysis of drug-susceptibility patterns and gene sequences associated with clarithromycin and amikacin resistance in serial Mycobacterium abscessus isolates from clinical specimens from Northeast Thailand

Ananta, Pimjai; Kham-ngam, Irin; Chetchotisakd, Ploenchan; Chaimanee, Prajuab; Reechaipichitkul, Wipa; Namwat, Wises; Lulitanond, Viraphong; Faksri, Kiatichai

doi:10.1371/journal.pone.0208053

Cited by 39 publications

(33 citation statements)

References 39 publications

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“…When the antimicrobial resistance rates obtained in the present study were compared with the results of previous studies, we found that there were high rates of resistance to multiple antimicrobials among the three MABC subspecies. The resistance rates regarding fluoroquinolones (CIP and MXF) and other broad-spectrum antibiotics (such as DOX, SXT, and MIN) were similar (all >70%) to those previously reported for MABC isolates in Taiwan Province of China, 23 as well as in Japan, 24 Thailand, 25 the USA, 26 and France. 27 In contrast, the rate of IPM resistance varied significantly among different studies.…”

Section: Discussionsupporting

confidence: 82%

Antimicrobial Susceptibility of Mycobacterium abscessus Complex Clinical Isolates from a Chinese Tertiary Hospital

Guo

Cao

et al. 2020

IDR

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Introduction: Mycobacterium abscessus complex (MABC) is a group of important infectious agents that are highly associated with drug resistance, and antibiotic treatment is usually ineffective. This study investigated the characteristics of antimicrobial susceptibility of MABC isolates and the synergy between certain β-lactam combinations against MABC infection. Methods: We collected 129 MABC isolates from patients with lower respiratory tract infections and categorized them into three subspecies. The minimum inhibitory concentrations (MICs) of 15 antimicrobials for the MABC isolates were determined using commercial Sensititre RAPMYCOI MIC plates and the broth microdilution method, as recommended in the CLSI (M24-A2). In addition, the MICs of imipenem, alone and with ceftazidime and/or avibactam, were assessed in vitro for all isolates. The erm(41) and rrl genes were also sequenced. Results: The MABC isolates exhibited >80% resistance to 11 of the 15 antimicrobials. Regarding the remaining four antimicrobials, the isolates were least resistant to tigecycline (12.4%) and amikacin (3.9%), and only partially resistant to two cefoxitin (39.5%) and imipenem (40.3%). Compared with M. massiliense isolates, M. abscessus and M. bolletii isolates were more resistant to amikacin and imipenem, whereas M. abscessus was significantly less resistant to tigecycline relative to M. massiliense and M. bolletii isolates. The clarithromycin inducible resistance rate was 68.4% and 74.3% among M. bolletii and M. abscessus isolates. Furthermore, 88.7% of the M. abscessus isolates carried a T at position 28 of erm(41), which is associated with inducible clarithromycin resistance. In addition, compared to imipenem with avibactam only, the MIC 50 and MIC 90 values of imipenem after adding ceftazidime plus avibactam were decreased fourfold. Conclusion: The antimicrobial resistance rates and the characteristics of the erm(41) gene associated with inducible clarithromycin resistance were different among the three MABC subspecies. There was also synergy between imipenem and 100μg/mL ceftazidime against MABC isolates.

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Section: Discussionsupporting

confidence: 82%

Antimicrobial Susceptibility of Mycobacterium abscessus Complex Clinical Isolates from a Chinese Tertiary Hospital

Guo

Cao

et al. 2020

IDR

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“…Specifically, M. abscessus subsp. abscessus and M. bolletii might harbour a functional gene erm(41) leading to lack of efficacy of macrolide; therein, raising questions on the utility of clarithromycin in the treatment of infections caused by these microorganisms with inducible macrolide resistance [18,27,28]. To investigate for this probability, gene sequencing is advisable.…”

Section: Discussionmentioning

confidence: 99%

“…e same issue emerged also for imipenem. Being so, while a prolonged incubation is recommended to test clarithromycin in vitro activity [19,29,30], a long incubation time can cause the inactivation of other antibiotics such as imipenem [28,30]. In light of this, the reported results might have been misleading.…”

Section: Discussionmentioning

confidence: 99%

Treating Primary Arthroprosthesis Infection Caused byMycobacterium abscessussubsp. abscessus

Pace

Antinolfi

Borroni

et al. 2019

Case Reports in Infectious Diseases

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Prosthetic joint infections (PJI) caused by nontuberculous mycobacteria are very rare, and results of treatment can be unpredictable. A 72-year-old female underwent hip replacement after an accidental fall in a local hospital in Santo Domingo. The postoperative period was uneventful except for a traumatic wound near the surgical scar. PJI caused by Mycobacterium abscessus subsp. abscessus was diagnosed 6 months later. A two-stage reimplantation was performed after a 3-month period of aetiology-directed therapy, including amikacin, imipenem, and clarithromycin. M. abscessus isolate was reported to be resistant to clarithromycin when incubation was protracted for 14 days and to harbour the gene erm(41). The patient manifested major side effects to tigecycline. At reimplant, microbiologic investigations resulted negative. Overall, medical treatment was continued for a 7-month period. When discontinued and at 6-month follow-up, the patient was clinically well, inflammatory markers were normal, and the radiography showed well-positioned prosthesis. Mycobacterium abscessus subsp. abscessus is a very rare cause of PJI, yet it must be included in the differential diagnosis, especially when routine bacteria cultures are reported being negative. Further investigations are needed to determine any correlations between clinical results and in vitro susceptibility tests, as well as the clinical implications of M. abscessus subsp. abscessus harbouring the functional gene erm(41). Moreover, investigations are needed for determine optimal timings of surgery and lengths of medical therapy to improve patient outcome.

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“…Currently there are no interpretative breakpoints available for meropenem, ertapenem and doripenem antibiotics for which those related to imipenem are considered [29]. Breakpoints for tigecycline were based on those used by Ananta [30].…”

Section: Methodsmentioning

confidence: 99%

Efficacy of carvacrol against resistant rapidly growing mycobacteria in the planktonic and biofilm growth mode

et al. 2019

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Rapidly growing mycobacteria (RGM) are environmental bacteria found worldwide with a propensity to produce skin and soft-tissue infections. Among them, the most clinically relevant species is Mycobacterium abscessus . Multiple resistance to antibiotics and the ability to form biofilm contributes considerably to the treatment failure. The search of novel anti-mycobacterial agents for the control of biofilm growth mode is crucial. The aim of the present study was to evaluate the activity of carvacrol (CAR) against planktonic and biofilm cells of resistant RGM strains. The susceptibility of RGM strains (n = 11) to antibiotics and CAR was assessed by MIC/MBC evaluation. The CAR activity was estimated by also vapour contact assay. The effect on biofilm formation and preformed biofilm was measured by evaluation of bacterial growth, biofilm biomass and biofilm metabolic activity. MIC values were equal to 64 μg/mL for most of RGM isolates (32–512 μg/mL), MBCs were 2–4 times higher than MICs, and MICs of vapours were lower (16 μg/mL for most RGM isolates) than MICs in liquid phase. Regarding the biofilm, CAR at concentrations of 1/2 × MIC and 1/4 × MIC showed a strong inhibition of biofilm formation (61–77%) and at concentration above the MIC (2–8 × MIC) produced significant inhibition of 4- and 8-day preformed biofilms. In conclusion, CAR could have a potential use, also in vapour phase, for the control of RGM.

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Analysis of drug-susceptibility patterns and gene sequences associated with clarithromycin and amikacin resistance in serial Mycobacterium abscessus isolates from clinical specimens from Northeast Thailand

Cited by 39 publications

References 39 publications

<p>Antimicrobial Susceptibility of <em>Mycobacterium abscessus</em> Complex Clinical Isolates from a Chinese Tertiary Hospital</p>

<p>Antimicrobial Susceptibility of <em>Mycobacterium abscessus</em> Complex Clinical Isolates from a Chinese Tertiary Hospital</p>

Treating Primary Arthroprosthesis Infection Caused byMycobacterium abscessussubsp. abscessus

Efficacy of carvacrol against resistant rapidly growing mycobacteria in the planktonic and biofilm growth mode

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