Analysis of Expressed Sequence Tags from the Marine Microalga Pseudochattonella farcimen (Dictyochophyceae)

Dittami, Simon M.; Riisberg, Ingvild; John, Uwe; Orr, Russell J. S.; Jakobsen, Kjetill S.; Edvardsen, Bente

doi:10.1016/j.protis.2011.07.004

Cited by 15 publications

(9 citation statements)

References 88 publications

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“…stramenopile oomycetes. However, a study has recently confirmed the presence of at least two different putative PKS III in Pseudochattonella farcimen from another class of ochrophytes, the Dichtyochophyta (Dittami et al, 2012b). These data corroborate the fact that a lateral transfer of PKS III gene has occurred after separation of diatoms from other ochrophytes, but before the divergence of brown algae with pelagophytes and dichtyochophytes.…”

Section: Discussionmentioning

confidence: 67%

Structure/Function Analysis of a Type III Polyketide Synthase in the Brown Alga Ectocarpus siliculosus Reveals a Biochemical Pathway in Phlorotannin Monomer Biosynthesis

et al. 2013

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ORCID ID: 0000-0001-7358-6282 (P.P.).Brown algal phlorotannins are structural analogs of condensed tannins in terrestrial plants and, like plant phenols, they have numerous biological functions. Despite their importance in brown algae, phlorotannin biosynthetic pathways have been poorly characterized at the molecular level. We found that a predicted type III polyketide synthase in the genome of the brown alga Ectocarpus siliculosus, PKS1, catalyzes a major step in the biosynthetic pathway of phlorotannins (i.e., the synthesis of phloroglucinol monomers from malonyl-CoA). The crystal structure of PKS1 at 2.85-Å resolution provided a good quality electron density map showing a modified Cys residue, likely connected to a long chain acyl group. An additional pocket not found in other known type III PKSs contains a reaction product that might correspond to a phloroglucinol precursor. In vivo, we also found a positive correlation between the phloroglucinol content and the PKS III gene expression level in cells of a strain of Ectocarpus adapted to freshwater during its reacclimation to seawater. The evolution of the type III PKS gene family in Stramenopiles suggests a lateral gene transfer event from an actinobacterium.

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Section: Discussionmentioning

confidence: 67%

Structure/Function Analysis of a Type III Polyketide Synthase in the Brown Alga Ectocarpus siliculosus Reveals a Biochemical Pathway in Phlorotannin Monomer Biosynthesis

et al. 2013

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“…Expressed sequence tags (EST) library has been analyzed in strains from P. farcimen (Dittami et al. ).…”

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confidence: 99%

“…Nuclear-, chloroplast-, and mitochondrial-encoded DNA sequences identified one strain isolated from German North Sea in 2000 as P. verruculosa, whereas strains from Skagerrak isolated in 2001 and 2006 belonged to P. farcimen (Riisberg and Edvardsen 2008). Expressed sequence tags (EST) library has been analyzed in strains from P. farcimen (Dittami et al 2012).…”

mentioning

confidence: 99%

Environmental Optima for Seven Strains of Pseudochattonella (Dictyochophyceae, Heterokonta)

Skjelbred

Edvardsen

Andersen

2012

Journal of Phycology

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The ichthyotoxic flagellate Pseudochattonella has formed recurrent blooms in the North Sea, Skagerrak and Kattegat since 1998. Five strains of Pseudochattonella farcimen and two strains of P. verruculosa were examined in an assay comparing the light response of specific growth rates over a range of temperatures and salinities to get further knowledge on the autecology of members of this genus. Temperature optima were lower in P. farcimen (9°C-15°C) than in P. verruculosa (12°C-20°C). P. farcimen also showed a somewhat lower salinity optimum (18-26) than P. verruculosa (20-32). All strains showed light-dependent growth responses reaching saturation between 18 and 52 μmol · photons · m(-2) · s(-1) at optimal temperature and salinity conditions. Compensation point estimates ranged from 4.2 to 15 μmol · photons · m(-2) · s(-1) . Loss rates increased with temperature and were lowest at salinities close to optimal growth conditions. Blooms of P. farcimen have been recorded in nature under conditions more similar to those minimizing loss rates rather than those maximizing growth rates in our culture study.

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“…; Dittami et al . ). All three examples demonstrate the importance of bacteria as actors that have shaped the evolutionary history of the algae they co‐existed with.…”

Section: Algae As ‘Superorganisms’ In a Changing Environmentmentioning

confidence: 97%

A metabolic approach to study algal–bacterial interactions in changing environments

2014

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Increasing evidence exists that bacterial communities interact with and shape the biology of algae and that their evolutionary histories are connected. Despite these findings, physiological studies were and still are generally carried out with axenic or at least antibiotic-treated cultures. Here, we argue that considering interactions between algae and associated bacteria is key to understanding their biology and evolution. To deal with the complexity of the resulting 'holobiont' system, a metabolism-centred approach that uses combined metabolic models for algae and associated bacteria is proposed. We believe that these models will be valuable tools both to study algal-bacterial interactions and to elucidate processes important for the acclimation of the holobiont to environmental changes.

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Analysis of Expressed Sequence Tags from the Marine Microalga Pseudochattonella farcimen (Dictyochophyceae)

Cited by 15 publications

References 88 publications

Structure/Function Analysis of a Type III Polyketide Synthase in the Brown Alga Ectocarpus siliculosus Reveals a Biochemical Pathway in Phlorotannin Monomer Biosynthesis

Structure/Function Analysis of a Type III Polyketide Synthase in the Brown Alga Ectocarpus siliculosus Reveals a Biochemical Pathway in Phlorotannin Monomer Biosynthesis

Environmental Optima for Seven Strains of Pseudochattonella (Dictyochophyceae, Heterokonta)

A metabolic approach to study algal–bacterial interactions in changing environments

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