Analysis of Flavonoids and Phenolic Acids in &lt;I&gt;Iris tectorum&lt;/I&gt; by HPLC-DAD-ESI-MS&lt;SUP&gt;n&lt;/SUP&gt;

Shu, Pan; Hong, Junli; Wu, Gang; Yu, Boyang; Qin, Min-Jian

doi:10.3724/sp.j.1009.2010.00202

Cited by 39 publications

(27 citation statements)

References 17 publications

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“…[3] Many isoflavones were named after the type of Iris from which they were common or firstly isolated. Identification of isoflavones and their derivatives, in the absence of standards, was achieved using the available literature on phytochemicals previously isolated or just identified in some of Iris spp, [5,7,13,30,[41][42][43] as well as by studying of its MS fragmentation pattern (exact mass and MS 4 fragmentation). Tables 1 and 2 summarized MS data for all isoflavone derivatives (compounds 32 -52) found in our Iris species.…”

Section: Isoflavones and Their Derivativesmentioning

confidence: 99%

Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above‐Ground Vegetative Parts and Flower of Three Iris Species

Kostić

Gašić

Pešić

et al. 2019

Chemistry & Biodiversity

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This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above‐ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC‐Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C‐glycosides, luteolin O‐glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.

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Section: Isoflavones and Their Derivativesmentioning

confidence: 99%

Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above‐Ground Vegetative Parts and Flower of Three Iris Species

Kostić

Gašić

Pešić

et al. 2019

Chemistry & Biodiversity

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“…The MS 2 spectra of compound 4 , 7 and 8 gave ions at m / z 463 and 301, originating from successive losses of 162 Da, suggesting the presence of two hexose residues, while the MS n information on compound 11 indicated the presence of one hexose residue. Compound 11 was unambiguously identified as tectoridin, which was confirmed by comparing with the reference standard compound (Shu et al , ; Ablajan, ). Compounds 4 , 7 and 8 , the three O ‐glycosides isomers, were differentiated by ESI‐MS n spectra in negative mode.…”

Section: Resultsmentioning

confidence: 99%

Qualitative and Quantitative Evaluation of Phenolic Compounds in Iris dichotoma Pall

Wei

Shu

Hong

et al. 2011

Phytochemical Analysis

Self Cite

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“…Various approaches have thus been proposed to use mass spectrometric fragmentations for structural characterization of flavonoids which are by far the most studied natural products by mass spectrometry [13,14,[36][37][38][39][40][41][42][43][44][45][46][47][48]. Furthermore the low-energy CID MS/MS spectra of various flavonoid aglycones and glycosides in mixture were described in both LC-ESI-MS/MS [30,31,49-51,] and LC-APCI-MS/MS [16,25].…”

Section: Application Of Positive and Negative Esi-ms Cid-ms And Tandmentioning

confidence: 99%

Mass Spectroscopic Methods for the Characterization of Flavonoid Compounds

Es‐Safi¹

2012

CBC

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Flavonoids are a broad class of polyphenolic secondary metabolites widely dispersed throughout the plant kingdom and found in substantial levels in commonly consumed fruits, vegetables and beverages. These compounds have aroused considerable interest because of their potential beneficial biochemical and antioxidant effects on human health. The structures of flavonoids are characterized by a fifteen-carbon backbone consisting of two aromatic and one oxygenated rings. Flavonoids could be divided into various types of monomeric aglycones, bi-, tri-, and oligo-flavonoids including proanthocyanidins.Owing to the importance of flavonoids, isolation and structural elucidation of natural flavonoids play an important role in many areas of science. This is generally performed by a combination of chromatographic and spectroscopic methods, including ultraviolet, nuclear magnetic resonance and mass spectrometry. Among all these techniques, mass spectrometry remains an important tool for the identification and structural determination of flavonoids. Its advantages are high sensitivity and possibilities of hyphenation with liquid chromatographic methods for the analysis of mixtures of compounds. Different desorption ionization methods allow the analysis of underivatized glycosides. Advances in mass spectrometry methodology have been shown to be extremely valuable for flavonoid analysis especially the use of mild ionization techniques, which have improved the possibility of recording molecular ions and suppressed the detection limits by several orders of magnitude. Detailed structural information can be obtained by resorting to cone voltage fragmentation and use of various types of mass analyzers.In this chapter, examples of the usefulness of mass spectroscopy techniques to the structural characterization of various flavonoids will be presented. In particular analysis of flavonoid aglycones and mono and di-O-glycosides, oligomeric and polymeric procyanidins using electropsray (ESI), matrix-assisted laser desorption ionization (MALDI), and tandem (MS/MS) mass spectrometry will be also presented.

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Analysis of Flavonoids and Phenolic Acids in <I>Iris tectorum</I> by HPLC-DAD-ESI-MS<SUP>n</SUP>

Cited by 39 publications

References 17 publications

Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above‐Ground Vegetative Parts and Flower of Three Iris Species

Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above‐Ground Vegetative Parts and Flower of Three Iris Species

Qualitative and Quantitative Evaluation of Phenolic Compounds in Iris dichotoma Pall

Mass Spectroscopic Methods for the Characterization of Flavonoid Compounds

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