Analysis of genes encoding highly conserved lysine‐rich proteins in <i>Aplysia californica</i> and <i>Saccharomyces cerevisiae</i>

Auclair, Daniel; Lang, B. Franz; Forest, Pierre; DesGroseillers, Luc

doi:10.1111/j.1432-1033.1994.tb18704.x

Cited by 7 publications

(7 citation statements)

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“…We originally isolated the nbl cDNA clone by virtue of its abundance in a Namalwa Burkitt Lymphoma cDNA library, 5 while the rat homologue fte‐1 was identified on the basis of its disruption in revertant v‐ fos ‐transformed Rat‐1 cells 18 . RPS3a homologues in other species have been identified on the basis of their abundant expression in feline leukaemia virus‐induced lymphomas (cat), 15 in the ovary (mosquito), 53 in neurons (sea hare), 54 during S‐phase (higher plants) 55 and following induction by TNF‐α (mouse) 56 . The original identification of a RPS3a homologue in yeast, as encoding a regulator of mitochondrial protein import, 57 has been disputed by other studies that have implicated the two yeast RPS3a homologues in cell cycle regulation 27 , 54 .…”

Section: ‘One Gene–dual Function’: a Widespread Phenomenonmentioning

confidence: 99%

Involvement of ribosomal proteins in regulating cell growth and apoptosis: Translational modulation or recruitment for extraribosomal activity?

1999

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Summary Gene recruitment is a mechanism of molecular evolution whereby a gene product can function in more than one distinct capacity. The 'one gene-dual function' phenomenon is well illustrated by crystallins, structural proteins that play both specialized roles in the eye lens and also 'housekeeping' enzyme roles. Ribosomal proteins are integral components of the basal cellular machinery involved in protein synthesis, whose roles have been regarded collectively as important, but individually somewhat mundane. However, various individual ribosomal proteins and also translation initiation and elongation factors have been found to play roles in regulating cell growth, transformation and death, giving rise to increasing speculation that components of the translational apparatus can act as multifunctional proteins. Recently, we have shown that ribosomal protein S3a (RPS3a) plays important roles in cell transformation and death, whereby constitutively or transiently enhanced RPS3a expression can be regarded as 'priming' a cell for apoptosis and suppression of such enhanced expression as 'execution'. While it is unclear whether RPS3a acts in a capacity mechanistically distinct from that in translation, such a possibility is discussed in this article in the light of recent, although not exhaustively reviewed, findings implicating the involvement of other individual ribosomal proteins in modulating and/or effecting changes in cellular responses and growth patterns in an extraribosomal capacity independent of their conventional role in translation.

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Section: ‘One Gene–dual Function’: a Widespread Phenomenonmentioning

confidence: 99%

Involvement of ribosomal proteins in regulating cell growth and apoptosis: Translational modulation or recruitment for extraribosomal activity?

1999

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“…2; Tables 1 and 2). Both of these S. cerevisiae genes have been given various names as a result of their various means of isolation or apparent functions: MFT1 (PLC2 and KRP-Y1) and PLC1 (CYC7) (2,16,24). However, Takakura and coworkers (57) have shown convincingly that FIG.…”

Section: Discussionmentioning

confidence: 99%

“…Two Rp10 genes exist in S. cerevisiae, MFT1 (also known as PLC2 and KRP-Y1) and PLC1 (CYC7) (2,16,24). Therefore, RP10 sequences in the C. albicans genome were investigated by Southern analysis (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…While the RP10 mRNA was not repressed by a mild heat shock at 37ЊC (Fig. 6), the levels of many S. cerevisiae mRNAs decrease transiently in response to such a treatment (21) and, in particular, the expression of an S. cerevisiae Rp10 gene is known to decrease dramatically at 39ЊC (2). Nevertheless, the tight regulation of the C. albicans RP10 mRNA during growth (Fig.…”

Section: Vol 177 1995mentioning

confidence: 99%

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Structure and regulation of a Candida albicans RP10 gene which encodes an immunogenic protein homologous to Saccharomyces cerevisiae ribosomal protein 10

et al. 1995

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, Infect. Immun. 61:4263-4271, 1993). cDNA10 was used to isolate its cognate gene, and both the cDNA and gene were sequenced, revealing a major open reading frame with the potential to encode a basic protein of 256 amino acids with a predicted molecular weight of 29 kDa. Over its entire length, the open reading frame showed strong homology at both the nucleic acid (75 to 78%) and amino acid (79 to 81%) levels to two Saccharomyces cerevisiae genes encoding the 40S ribosomal protein, Rp10. Therefore, our C. albicans gene was renamed RP10. Northern (RNA) analyses in C. albicans 3153 revealed that RP10 expression is regulated in a manner very similar to that of S. cerevisiae ribosomal genes. The level of the RP10 mRNA decreased upon heat shock (from 25 to 45؇C) and was tightly regulated during growth. Maximal levels of the mRNA were reached during mid-exponential phase before they decreased to negligible levels in stationary phase. The level of the RP10 mRNA was induced only transiently during the yeast-to-hyphal morphological transition but did not appear to respond to hyphal development per se.Candida albicans is a commensal fungus of humans which can cause irritating infections of the epithelial tissues, particularly in the mouth and urogenital tract (39). In the immunocompromised host, it can cause deep-seated and systemic infections, which may be life threatening. Little is known about the relative importance of the various potential virulence factors of C. albicans during pathogenesis. These factors include the production of extracellular hydrolytic enzymes, especially proteases and phospholipases (5,10,22,26,32,41), the ability to adhere to host tissues (8), the immunomodulatory effects of various cell wall components (34,58), and the ability to undergo a dimorphic transition from a budding yeast to a hyphal form (39,52). The relationship between the dimorphic transition and pathogenicity remains unclear (10,33,39,42,51). Both forms are found in infected tissues (40, 42), but C. albicans hyphae appear to be better adapted to penetrate epithelia (48) and therefore might play a role in establishing deepseated infections.Although numerous factors can stimulate C. albicans dimorphism in vitro (39), little is known about the mechanisms which control the transition. Different approaches are being taken to identify genes involved in the control of dimorphism. For example, the identification of S. cerevisiae genes that influence pseudohyphal growth in this yeast (17, 30) may lead ultimately to the isolation of homologs that are important for dimorphism in C. albicans. Also, differential hybridization strategies are proving successful in the identification of genes which are regulated during dimorphism despite the fact that the expression of many genes fluctuates during the yeast-to-hyphal form transition (54, 55). Such strategies have led to the isolation of the C. albicans ECE1 and PHR1 genes, which are expressed specifically in the hyphal growth form (4, 14), but these genes are not required for the transition unde...

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“…M88335; Gordon et al, 1992); Aplysia (Ac.Num. X68555; Auclair et al, 1994). Amino acids are represented by the standard single-letter code.…”

Section: Sequence Analysis and Genomic Organization Of Clonementioning

confidence: 99%

**Cloning and characterization of cDNAs preferentially expressed in the ovary of the mosquito,*Anopheles gambiae***

Zurita

Reynaud

Kafatos

1997

Insect Molecular Biology

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We used differential screening to isolate from an ovarian cDNA library two expressed sequences that are enriched substantially in ovaries of blood-fed female Anopheles gambiae, as compared to female carcass and male mosquitoes. One of these clones encodes an isoform of histone H2B, whose transcript is polyadenylated at the 3' end. The other cDNA clone encodes a protein that is highly conserved in evolution and has been implicated in growth control although its function is still obscure. Both genes can be used to study gene activation during An. gambiae oogenesis.

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Analysis of genes encoding highly conserved lysine‐rich proteins in Aplysia californica and Saccharomyces cerevisiae

Cited by 7 publications

References 18 publications

Involvement of ribosomal proteins in regulating cell growth and apoptosis: Translational modulation or recruitment for extraribosomal activity?

Involvement of ribosomal proteins in regulating cell growth and apoptosis: Translational modulation or recruitment for extraribosomal activity?

Structure and regulation of a Candida albicans RP10 gene which encodes an immunogenic protein homologous to Saccharomyces cerevisiae ribosomal protein 10

**Cloning and characterization of cDNAs preferentially expressed in the ovary of the mosquito,*Anopheles gambiae***

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Analysis of genes encoding highly conserved lysine‐rich proteins in Aplysia californica and Saccharomyces cerevisiae

Cited by 7 publications

References 18 publications

Involvement of ribosomal proteins in regulating cell growth and apoptosis: Translational modulation or recruitment for extraribosomal activity?

Involvement of ribosomal proteins in regulating cell growth and apoptosis: Translational modulation or recruitment for extraribosomal activity?

Structure and regulation of a Candida albicans RP10 gene which encodes an immunogenic protein homologous to Saccharomyces cerevisiae ribosomal protein 10

Cloning and characterization of cDNAs preferentially expressed in the ovary of the mosquito,Anopheles gambiae

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**Cloning and characterization of cDNAs preferentially expressed in the ovary of the mosquito,*Anopheles gambiae***