Analysis of Genetically Modified Food Using High-Performance Separation Methods

Trojanowicz, Marek; Latoszek, Anna; Poboży, Ewa

doi:10.1080/00032711003653841

Cited by 8 publications

(11 citation statements)

References 92 publications

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“…At present, determination of genetically modified foods is carried out using methods involving the determination of specific DNA fragments or checking the quality and quantity of proteins []. For determination of DNA fragments, polymerase chain reaction (PCR) is used, common method from molecular biology, allowing for accurate detection of DNA sequences introduced in the modified plant [].…”

Section: Introductionmentioning

confidence: 99%

“…For determination of DNA fragments, polymerase chain reaction (PCR) is used, common method from molecular biology, allowing for accurate detection of DNA sequences introduced in the modified plant []. Determination of proteins for detection of genetic modification of plant material is carried out also using immunochemical methods where individual proteins are identified [] or non‐target profiling analysis methods including proteomics approaches [], based on a highly efficient method for separation and mass spectrometry []. Such studies are an essential part of risk assessment of genetically modified food [].…”

Section: Introductionmentioning

confidence: 99%

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Low‐molecular weight protein profiling of genetically modified maize using fast liquid chromatography electrospray ionization and time‐of‐flight mass spectrometry

Koc

Cañuelo

García-Reyes

et al. 2012

J of Separation Science

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In this work, the use of liquid chromatography coupled to electrospray time-of-flight mass spectrometry (LC-TOFMS) has been evaluated for the profiling of relatively low-molecular weight protein species in both genetically modified (GM) and non-GM maize. The proposed approach consisted of a straightforward sample fractionation with different water and ethanol-based buffer solutions followed by separation and detection of the protein species using liquid chromatography with a small particle size (1.8 μm) C(18) column and electrospray-time-of-flight mass spectrometry detection in the positive ionization mode. The fractionation of maize reference material containing different content of transgenic material (from 0 to 5% GM) led to five different fractions (albumins, globulins, zeins, zein-like glutelins, and glutelins), all of them containing different protein species (from 2 to 52 different species in each fraction). Some relevant differences in the quantity and types of protein species were observed in the different fractions of the reference material (with different GM contents) tested, thus revealing the potential use of the proposed approach for fast protein profiling and to detect tentative GMO markers in maize.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low‐molecular weight protein profiling of genetically modified maize using fast liquid chromatography electrospray ionization and time‐of‐flight mass spectrometry

Koc

Cañuelo

García-Reyes

et al. 2012

J of Separation Science

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“…Other techniques as gas chromatography (GC) still have an important role in food analysis, for instance, for the analysis of volatile fractions or fatty acids in foods [20]. Electrodriven separation techniques such as capillary electrophoresis (CE) or microchip capillary electrophoresis have found important applications in food analysis as can be deduced from the many review works devoted to this topic [21,22], including the detection of genetically modified organisms [23,24], nucleosides and nucleotides in foods [25], analysis of contaminants in emerging food safety issues and food traceability [26], and food-borne pathogens [27].…”

Section: Food Analysis: Current State Of the Art Methodologies And mentioning

confidence: 99%

Food Analysis: Present, Future, and Foodomics

Cifuentes

2012

ISRN Analytical Chemistry

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This paper presents a revision on the instrumental analytical techniques and methods used in food analysis together with their main applications in food science research. The present paper includes a brief historical perspective on food analysis, together with a deep revision on the current state of the art of modern analytical instruments, methodologies, and applications in food analysis with a special emphasis on the works published on this topic in the last three years (2009)(2010)(2011). The article also discusses the present and future challenges in food analysis, the application of "omics" in food analysis (including epigenomics, genomics, transcriptomics, proteomics, and metabolomics), and provides an overview on the new discipline of Foodomics.

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“…On the other hand, CE has proven its potential for profiling providing complementary information with respect to other applied methodologies as liquid chromatography (LC)-MS [ 17 ]. Current trends in the use of high-performance separation methods for the analysis of genetically modified foods have been reviewed [ 18 ]. In 2009, an overview focused on the applications of capillary electrophoresis and microchip capillary electrophoresis to the detection of GMOs was published [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis

Vega

Marina

2014

IJMS

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Advances in biotechnology have increased the demand for suitable analytical techniques for the analysis of genetically modified organisms. Study of the substantial equivalence, discrimination between transgenic and non-transgenic cultivars, study of the unintended effects caused by a genetic modification or their response to diverse situations or stress conditions (e.g., environmental, climatic, infections) are some of the concerns that need to be addressed. Capillary electrophoresis (CE) is emerging as an alternative to conventional techniques for the study and characterization of genetically modified organisms. This article reviews the most recent applications of CE for the analysis and characterization of transgenic cultivars in the last five years. Different strategies have been described depending on the level analyzed (DNA, proteins or metabolites). Capillary gel electrophoresis (CGE) has shown to be particularly useful for the analysis of DNA fragments amplified by PCR. Metabolites and proteins have been mainly separated using capillary zone electrophoresis (CZE) using UV and MS detection. Electrophoretic chips have also proven their ability in the analysis of transgenic cultivars and a section describing the new applications is also included.

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Analysis of Genetically Modified Food Using High-Performance Separation Methods

Cited by 8 publications

References 92 publications

Low‐molecular weight protein profiling of genetically modified maize using fast liquid chromatography electrospray ionization and time‐of‐flight mass spectrometry

Low‐molecular weight protein profiling of genetically modified maize using fast liquid chromatography electrospray ionization and time‐of‐flight mass spectrometry

Food Analysis: Present, Future, and Foodomics

Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis

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