Analysis of Glycerophosphates. II. Determination of Small Amounts of Orthophosphates in Glycerophosphates**Scientific Section, A Ph. A., Dallas meeting, 1936.

Analyses of Sodium Meta-, Pyro-, and Orthophosphates: WIth Some Annotations on Methods

“…It seemed desirable, therefore, to develop a simple method which would permit the isolation of a characteristic derivative of succinic acid in order to provide both qualitative and quantitative evidence of the tissue composition. Auwers, in 1896 (8), observed that anhydrides of organic acids condense with certain aromatic amines to give insoluble crystalline derivatives. Succinic acid is readily converted into its anhydride when heated with acetyl chloride (6), and the condensation product of succinic anhydride with p-toluidine mentioned by Auwers is a well-crystallized substance that is almost insoluble in toluene and possesses excellent properties for quantitative isolation and for identification.…”