Analysis of hematologic alterations, immune responses and metallothionein gene expression in Nile tilapia (<i>Oreochromis niloticus</i>) exposed to silver nanoparticles

Thummabancha, Kubpaphas; Onparn, Nuttaphon; Srisapoome, Prapansak

doi:10.1080/1547691x.2016.1242673

Cited by 51 publications

(18 citation statements)

References 41 publications

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“…Similarly, the evaluation of haematological parameters is also considered an essential criterion in assessing nanomaterials' toxicity. [ 39 ] Therefore, the haematological analysis performed after the chronic exposure period illustrated no significant alterations of haematological parameters in EHN‐administered mice than the control group, except the elevation of neutrophils. Further, histopathology analysis of H&E stained organ tissue sections demonstrated normal anatomy of vital organs (heart, brain, spleen, liver and thymus) in control and EHN‐treated groups.…”

Section: Discussionmentioning

confidence: 91%

Europium Hydroxide Nanorods Mitigate Hind Limb Ischemia in Wistar Rats

Nethi

Barui

Jhelum

et al. 2021

Advanced Therapeutics

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Lack of adequate blood supply to the limb extremities results in critical limb ischemia (CLI) and subsequent tissue death. Conventional approaches for CLI treatment include bypass surgery and angioplasty, which achieved limited clinical success due to their complex invasiveness. Of late, growth factor and cell‐based therapies emerged as alternative treatment strategies for CLI. However, these strategies could not achieve much success due to the associated drawbacks. Earlier, this study group develops europium hydroxide nanorods (EHN) which demonstrates potential proangiogenic (stimulating new blood vessel growth) properties. Recently it is shown that EHN could ameliorate the myocardial ischemia in Wistar rats by promoting therapeutic angiogenesis and mitigate cadmium‐induced vascular toxicity. Considering the importance of revascularization in managing CLI, it is hypothesized that the proangiogenic EHN might be useful for its effective treatment. Hence, in the present study, the therapeutic efficacy of EHN is comprehensively evaluated in the hind limb ischemia model of Wistar rats. The EHN‐administered ischemic rats exhibit improved motility, enhanced blood flow to the ischemic limb along with increased expression of angiogenic factors. Additionally, toxicity and pharmacokinetic studies of EHN in mice demonstrate their nontoxic nature, suggesting the feasibility of their practical clinical application for CLI treatment.

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Section: Discussionmentioning

confidence: 91%

Europium Hydroxide Nanorods Mitigate Hind Limb Ischemia in Wistar Rats

Nethi

Barui

Jhelum

et al. 2021

Advanced Therapeutics

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“…Changes in hematological parameters have been reported in fish exposed both to mixtures of pollutants and to single substances (Bacchett et al 2011;Seriani et al 2013;Fujimoto et al 2012;Carraschi et al 2016;Cruz et al 2015;Thummabancha et al 2016;Kumar et al 2017). Low frequencies of erythroblasts and thrombocytes could be explained by water contaminants generating intrasplenic and intrahepatic damage, thus decreasing hematopoiesis.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in functional enzymes (Bonnail et al 2016;Vieira et al 2017;Sadauskas-Henrique et al 2017), effects of cytogenotoxicity (Prado, et al 2014, de Almeida Duarte et al 2016, mutagenicity (Araújo al. 2014;Weldetinsae et al 2017), and hematological, immunological, and histopathological changes (da Cruz et al, 2015;Corrêa et al 2017;Thummabancha et al 2016;Hedayati and Darabitabar 2017) all represent well-documented biomarkers used in environmental monitoring.…”

Section: Introductionmentioning

confidence: 99%

Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond

Silva

Corrêa

Abessa

et al. 2017

Environ Sci Pollut Res

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The present study evaluated the water quality of a polluted pond through the analysis of in vitro mucociliary transport, hematological parameters, and biomarkers of cyto-genotoxicity in the Nile tilapia (Oreochromis niloticus). Blood and mucus samples were collected from ten specimens from the polluted pond and from ten specimens from a control area. The fish were anesthetized with 3% benzocaine, mucus was collected directly from the gills, and blood was drawn from the caudal artery. Blood smears were stained using the May-Grünwald Giemsa process for the differential leukocyte counts and to determine the frequency of leukocytes, thrombocytes, erythroblasts, micronuclei, and nuclear abnormalities. The results revealed low transportability in vitro, a high percentage of monocytes and eosinophils, and increased frequency of leukocytes and nuclear abnormalities in fish from the polluted pond. However, the frequency of thrombocytes and erythroblasts and the percentage of lymphocytes and neutrophils were significantly lower. It is possible to conclude that changes in fish are due to poor water quality and that these non-destructive biomarkers can be used for the biomonitoring of aquatic environments vulnerable to contamination.

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“…Previous studies have demonstrated that AgNPs are toxic to all tested marine organisms in a dose-dependent manner, indicating that AgNPs may have negative effects on marine organisms at different trophic levels of the marine environment. Immunotoxic effects of AgNPs have been demonstrated in some aquatic animals such as Nile tilapia and mussel ( Gagne et al, 2013 ; Thummabancha, Onparn & Srisapoome, 2016 ). Nevertheless, to date the potential toxicity of AgNPs on marine mammals such as cetaceans has not been sufficiently studied.…”

Section: Introductionmentioning

confidence: 99%

Th2 cytokine bias induced by silver nanoparticles in peripheral blood mononuclear cells of common bottlenose dolphins (Tursiops truncatus)

Wang

Chang

et al. 2018

PeerJ

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BackgroundSilver nanoparticles (AgNPs) have been widely used in many commercial products due to their excellent antibacterial ability. The AgNPs are released into the environment, gradually accumulate in the ocean, and may affect animals at high trophic levels, such as cetaceans and humans, via the food chain. Hence, the negative health impacts caused by AgNPs in cetaceans are of concern. Cytokines play a major role in the modulation of immune system and can be classified into two types: Th1 and Th2. Th1/Th2 balance can be evaluated by the ratios of their polarizing cytokines (i.e., interferon [IFN]-γ/Interleukin [IL]-4), and animals with imbalanced Th1/Th2 response may become more susceptible to certain kinds of infection. Therefore, the present study evaluated the in vitro cytokine responses of cetacean peripheral blood mononuclear cells (cPBMCs) to 20 nm citrate-AgNPs (C-AgNP20) by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).MethodsBlood samples were collected from six captive common bottlenose dolphins (Tursiops truncatus). The cPBMCs were isolated and utilized for evaluating the in vitro cytokine responses. The cytokines evaluated included IL-2, IL-4, IL-10, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. The geometric means of two housekeeping genes (HKGs), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and β2-microglobulin (B2M), of each sample were determined and used to normalize the mRNA expression levels of target genes.ResultsThe ratio of late apoptotic/necrotic cells of cPBMCs significantly increased with or without concanavalin A (ConA) stimulation after 24 h of 10 µg/ml C-AgNP20 treatment. At 4 h of culture, the mRNA expression level of IL-10 was significantly decreased with 1 µg/ml C-AgNP20 treatment. At 24 h of culture with 1 µg/ml C-AgNP20, the mRNA expression levels of all cytokines were significantly decreased, with the exceptions of IL-4 and IL-10. The IFN-γ/IL-4 ratio was significantly decreased at 24 h of culture with 1 µg/ml C-AgNP20 treatment, and the IL-12/IL-4 ratio was significantly decreased at 4 or 24 h of culture with 0.1 or 1 µg/ml C-AgNP20 treatment, respectively. Furthermore, the mRNA expression level of TNF-α was significantly decreased by 1 µg/ml C-AgNP20 after 24 h of culture.DiscussionThe present study demonstrated that the sublethal dose of C-AgNP20 (≤1 µg/ml) had an inhibitory effect on the cytokine mRNA expression levels of cPBMCs with the evidence of Th2 cytokine bias and significantly decreased the mRNA expression level of TNF-α. Th2 cytokine bias is associated with enhanced immunity against parasites but decreased immunity to intracellular microorganisms. TNF-α is a contributing factor for the inflammatory response against the infection of intracellular pathogens. In summary, our data indicate that C-AgNP20 suppresses the cellular immune response and thereby increases the susceptibility of cetaceans to infection by intracellular microorganisms.

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Analysis of hematologic alterations, immune responses and metallothionein gene expression in Nile tilapia (Oreochromis niloticus) exposed to silver nanoparticles

Cited by 51 publications

References 41 publications

Europium Hydroxide Nanorods Mitigate Hind Limb Ischemia in Wistar Rats

Europium Hydroxide Nanorods Mitigate Hind Limb Ischemia in Wistar Rats

Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond

Th2 cytokine bias induced by silver nanoparticles in peripheral blood mononuclear cells of common bottlenose dolphins (Tursiops truncatus)

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