Analysis of HLA-DRB1*1501 in Multiple Sclerosis Patients in Khuzestan Province, Iran

Abstract

Background: Multiple sclerosis (MS) is a demyelinating, autoimmune and neurodegenerative disease of the central nervous system (CNS). The pathogenesis of this disease is still unknown, although there are evidences of environmental factors affecting subjects with genetic predisposition factors. The contribution of HLA-DRB1*1501 to MS risk has been replicated and confirmed in most population-based studies. Since there are no data with respect to the association of HLA-DRB1*1501 and MS in Khuzestan province. Objectives: The aim of this study was to investigate the correlation of this allele with MS in Khuzestan province. Methods: In this case-control study, DRB1*1501 allele was analyzed by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 200 MS patients and 200 unrelated healthy individuals, without any autoimmune disease from the same geographical region. The frequencies of the mentioned allele were compared between the patients and control group using SPSS 16 statistical software and the chi square test. Results:The results demonstrated that distribution of DRB1*1501 allele was statistically different between patient and control (41.5% vs. 22.81%, P < 0.001); significant correlation was observed among these allele with both Arab (28.84% vs. 50.84%, P = 0.005) and Persian (15.87% vs. 35.55%, P = 0.018) ethnicities; although no association was revealed between mentioned allele and disease clinical course (relapsing-remitting, secondary-progressive, progressive-relapsing and primary-progressive), expanded disability scale score (EDSS) and gender. Conclusions:The study presents association of susceptibility to multiple sclerosis in southwest of Iran with HLA-DRB1*1501 allele in both Arab and Persian ethnic; although DRB1*1501 may be is not involved in the pathogenesis of different MS disease subtypes. The results are consistent with most of the other studies in Iran; and also most studies in European populations.

Association Between HLA-A*02 Genotype and Multiple Sclerosis in Khuzestan Province, Iran

“…For this reason, investigation on MS is necessary in Iran. Frequencies of class II of HLA include HLA-DQB1*0602, -DRB1*1501, and -DRB5*01 variants in the MS population of Khuzestan Province were already reported; association of HLA-DRB1*1501 and no association of HLA DRB5*01 and HLA-DQB1*0602 with MS in the population were observed (28)(29)(30). The current study investigated the association between HLA-A*02, an important type of HLA-A gene of HLA class I in the diagnosis or pathogenesis of MS, and MS in Khuzestan Province, Iran.…”

Association of human leukocyte antigen-DRB haplotype in multiple sclerosis population of Khuzestan, Iran

“…The MOG and DRBI*1501 primers were demonstrated in a previous study. 2 The result validation was done by sequencing selected samples randomly.…”

“…The HLA-DRB1*1501 and HLA-DRB5*05 frequency were already published. 2 , 17 The percentage of HLA-DRB1*1501-DRB5*05 haplotype was calculated. Fisher’s exact and chi-square tests were used for comparison of the named haplotype percentage in the case and control groups.…”

“…In particular, MS association with the human leukocyte antigen (HLA) genes from the MHC class II, DRB1*1501 has consistently been found. 2…”

“…So far, several studies have been carried out on the association of DRB1*1501 and MS in Iran. 2 , 8 - 11 Also, there is a high spectrum of variation in Khuzestan Province, Iran, in neighborhood of the Arabic countries and the Persian Gulf due to high migration rate from different ethnic populations. 12 , 13 As there is no study so far about HLA-DRB1*1501-DRB5*01 correlation with the mentioned disease and given the ethnic variation as well as based on the fact that Iranian Arabs are mostly located in Khuzestan Province, this survey might be of great importance.…”