Analysis of Human and Microbial Salivary Proteomes in Children Offers Insights on the Molecular Pathogenesis of Molar-Incisor Hypomineralization

Abstract

Molar incisor hypomineralization is a complex developmental enamel defect that affects the permanent dentition of children with significant functional and aesthetic implications. Saliva is an ideal diagnostic tool and ensures patients’ compliance by diminishing the discomfort especially in pediatric population. Lately, salivary proteome analysis has progressively evolved in various biomedical disciplines. As changes in saliva composition are associated with oral diseases, it is reasonable to assume that the saliva proteome of MIH-affected children might be altered compared to healthy children. This study analyzed the human and microbial salivary proteome in children with MIH in order to identify salivary markers indicative of the pathology. The conducted proteomic analysis generated a comprehensive dataset comprising a total of 1515 high confidence identifications and revealed a clear discrimination between the two groups. Statistical comparison identified 142 differentially expressed proteins, while the pathway analysis indicated deregulation of inflammation, immune response mechanisms, and defense response to bacteria in MIH patients. Bacterial proteome analysis showed a lower diversity for the microbial species, which highlights the dysbiotic environment established in the MIH pathology.

Revalence of Molar Incisor Hypomineralization among Primary School Children in Baquba City

“…Results shows a low percentage of extracted teeth due to MIH and these findings agrees with findings of previous study [41], this is might be related due to the devolving in the process of early detection of the MIH and the management of condition by preventive and conservative techniques . These finding do agree with the findings of previous studies, demarcated opacity were the most commen type of the defect with significant association, [42,43], this might be explained in part by the inclusion of younger children in the present research, since a number of those delineated opacities may break with period of time. This theory is backed by past research findings [44].…”

Revalence of Molar Incisor Hypomineralization among Primary School Children in Baquba City

“…Results shows a low percentage of extracted teeth due to MIH and these findings agrees with findings of previous study [41], this is might be related due to the devolving in the process of early detection of the MIH and the management of condition by preventive and conservative techniques . These finding do agree with the findings of previous studies, demarcated opacity were the most commen type of the defect with significant association, [42,43], this might be explained in part by the inclusion of younger children in the present research, since a number of those delineated opacities may break with period of time. This theory is backed by past research findings [44].…”

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

“…Recently, Pappa et al [ 165 ] analysed the human and microbial salivary proteome of children with molar incisor hypomineralisation (MIH) to identify salivary biomarkers of pathology. The study involved 10 children diagnosed with MIH and 10 healthy children (control sample).…”