Analysis of hydroquinone and some of its ethers by using capillary electrochromatography

Desiderio, Claudia; Ossicini, L.; Fanali, Salvatore

doi:10.1016/s0021-9673(99)01197-8

Cited by 56 publications

(25 citation statements)

References 15 publications

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“…[7][8] An extensive literature survey revealed Spectrophometric [9][10][11] , TLC [12] and HPLC [13][14][15] for the determination of Hydroquinone and the reported analytical procedure for the estimation of monobenzone in bulk sample and unit dosage forms include HPLC [16][17][18][19] , TLC [20] and Electrophoresis techniques. [21] But there is no method which describes the simultaneous determination of Hydroquinone and Monobenzone from cream dosage form meant for external application.…”

Section: Molecular Structure Of Hydroquinonementioning

confidence: 99%

Development of Analytical Method for Simultaneous Estimation of Hydroquinone and Monobenzone in Topical Formulation by Rp-HPLC

Barange¹

2017

WJPR

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A new specific, precise, accurate and robust RP-HPLC method has been developed for the simultaneous determination of Hydroquinone and Monobenzone in a pharmaceutical cream formulation. The chromatographic separation was carried out at Analytical technology HPLC instrument (Software: HPLC Work Station) equipped with Deuterium lamp as detector, HPLC pump and manual injecting facility programmed at 20 μL capacity per injection was used. The stationary phase was Zodiac Column C 18 (250 mm x 4.6 mm, 5μm) at ambient temperature. The mobile phase was Acetonitrile: Triethylamaine Buffer (60:40) adjust pH 3.5 by 10% orthophosphoric acid. Detection was carried out at 230nm using UV Detector. The flow rate was 1.0mL/min and retention time was about 3.3mins and 5.1mins for Hydroquinone and Monobenzone. The linearity was obtained in the concentration range of 24-64µg/mL and 30-70µg/mL for Hydroquinone and Monobenzone respectively. Mean percentage recoveries were 99.89% for Hydroquinone and 99.57% for Monobenzone. The LOD of Hydroquinone and Monobenzone was found to be 1 μg/mL and 2.0μg/mL whereas the LOQ was 5μg/mL and 10 μg/mL respectively. The assay values of both the analytes was found to be well within the limits that is 100.05% and 99.55%for Hydroquinone and Monobenzone respectively. Percentage relative standard deviation of percent assay values for replicate sample preparation was 1.09% for Hydroquinone and 0.96% for Monobenzone. The method was robust with respect to change in flow rate, and composition of mobile phase.

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Section: Molecular Structure Of Hydroquinonementioning

confidence: 99%

Development of Analytical Method for Simultaneous Estimation of Hydroquinone and Monobenzone in Topical Formulation by Rp-HPLC

Barange¹

2017

WJPR

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“…In the present study, NLC formulation of HQ was prepared that provides effective treatment in hyper pigmentation and melasma by passing the major obstacle in skin permeation. A detailed literature survey regarding existing methods of analysis like high-performance liquid chromatography (HPLC) [10], thin layer chromatography (TLC) [11], micellar electrokinetic chromatography (MEKC) [12], and capillary electrochromatography (CEC) techniques [13] in various biological matrices revealed that these methods are expensive, more sophisticated and involves extensive skills, thus there is need to develop simple spectrophotometric method for the estimation of Hydroquinone in various dosage forms. There is one reported UV spectrophotometric method for the estimation of hydroquinone individually in liposomes using methanol [14] and other UV spectrophotometric method for the simultaneous estimation of tazarotene and hydroquinone in gel preparation [15].…”

Section: Fig 1: Chemical Structure Of Hydroquinonementioning

confidence: 99%

Development and Validation of Uv-Spectrophotometric Method for Estimation of Hydroquinone in Bulk, Marketed Cream and Preapared NLC Formulation

Kaur

et al. 2017

Int J App Pharm

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Objective: The aim of the present work was to develop a simple, rapid, accurate and economical UV-visible spectrophotometric method for the determination of hydroquinone (HQ) in its pure form, marketed formulation as well as in the prepared nanostructured lipid carrier (NLC) systems and to validate the developed method.Methods: HQ was estimated at UV maxima of 289.6 nm in pH 5.5 phosphate buffer using UV-Visible double beam spectrophotometer. Following the guidelines of the International Conference on Harmonization (ICH), the method was validated for various analytical parameters like linearity, precision, and accuracy robustness, ruggedness, limit of detection, quantification limit, and formulation analysis. Results:The obtained results of the analysis were validated statistically. Recovery studies were performed to confirm the accuracy of the proposed method. In the developed method, linearity over the concentration range of 5-40 μg/ml of HQ was observed with the correlation coefficient of 0.998 and found in good agreement with Beer Lambert's law. The precision (intra-day and inter-day) of the method was found within official RCD limits (RSD<2%). Conclusion:The sensitivity of the method was assessed by determining the limit of detection and limit of quantification. It could be concluded from the results obtained that the purposed method for estimation of HQ in pure form, in the marketed ointment and in the prepared NLC-formulation was simple, rapid, accurate, precise and economical. It can be used successfully in the quality control of pharmaceutical formulations and for the routine laboratory analysis.

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“…al., 1999), capillary electrochromatography (Desiderio et al, 2000), micellar chromatography (Sakodinskaya et al, 1992) and other analytical techniques (Wang, 1995;Cruz, Fatibello-Filho, 2000;Rueda et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Determination of optimum wavelength and derivative order in spectrophotometry for quantitation of hydroquinone in creams

García¹,

Santoro²,

Singh³

et al. 2007

Rev. Bras. Cienc. Farm.

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Analysis of hydroquinone and some of its ethers by using capillary electrochromatography

Cited by 56 publications

References 15 publications

Development of Analytical Method for Simultaneous Estimation of Hydroquinone and Monobenzone in Topical Formulation by Rp-HPLC

Development of Analytical Method for Simultaneous Estimation of Hydroquinone and Monobenzone in Topical Formulation by Rp-HPLC

Development and Validation of Uv-Spectrophotometric Method for Estimation of Hydroquinone in Bulk, Marketed Cream and Preapared NLC Formulation

Determination of optimum wavelength and derivative order in spectrophotometry for quantitation of hydroquinone in creams

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