Analysis of IgG Subclass and IgE Response in Allergic Disease Caused by &lt;i&gt;Aspergillus fumigatus&lt;/i&gt; by Immunoblotting Techniques

J, Trompelt; Becker, W.-M.; Schlaak, M

doi:10.1159/000236697

Cited by 19 publications

(10 citation statements)

References 20 publications

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“…According to Trompelt et al . major allergens of A. fumigatus recognized by IgE antibodies in sera from seven asthmatic patients have molecular weights of 20, 29, 34, 38 and 45 kDa [29]. Proteins recognized with IgE in sera from six patients of ABPA have molecular weights of 14–45 kDa [29].…”

Section: Discussionmentioning

confidence: 99%

Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N‐terminal amino acid sequence analysis

Shen

Lin

Tam

et al. 2001

Clin Experimental Allergy

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Aspergillus species are common airborne fungi that have been identified as causative agents of extrinsic bronchial asthma. More than 10 allergens from A. fumigatus have been recently characterized by cDNA cloning. The objective of this study is to identify A. fumigatus allergens through immunoblot analysis using sera from asthmatic patients. IgE-binding components of A. fumigatus and IgE cross-reactivity among allergens of different prevalent airborne fungal species were analysed by immunoblot and immunoblot inhibition, respectively, using sera from asthmatic patients. The N-terminal amino acid sequences of major allergens identified were determined by Edman degradation. Among two batches (70 and 41 sera) of asthmatic sera tested, 19 (27%) and 14 (34%), respectively, have IgE immunoblot reactivity towards components of A. fumigatus. A 34-kDa protein that reacts with IgE antibodies in 15 (79%) and 11 (79%) of the 19 and 14 positive samples, respectively, may be considered a major allergen of A. fumigatus. The N-terminal amino acid sequences of the 34 kDa major allergen and the 30.5 and 30 kDa IgE-binding components of A. fumigatus showed sequence identity to that of the vacuolar serine proteinase from A. fumigatus. The results from immunoblot inhibition show IgE cross-reactivity among major allergens of A. fumigatus, P. notatum and P. oxalicum. Results obtained suggest that the 34 kDa major allergen of A. fumigatus may be a vacuolar serine proteinase. There is IgE cross-reactivity among serine proteinase allergens of A. fumigatus, P. notatum and P. oxalicum.

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Section: Discussionmentioning

confidence: 99%

Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N‐terminal amino acid sequence analysis

Shen

Lin

Tam

et al. 2001

Clin Experimental Allergy

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“…The heightened total and specific anti-Aspergillus IgE antibody responses have been described by several groups [14,18,[143][144][145][146][147]. In ABPA, there are also increased amounts of IgG and IgA anti-Aspergillus antibodies, which reflect the Th2 humoral versus Th1 cellular response to Aspergillus antigens in these patients [148][149][150][151][152][153][154][155]. Although other Aspergillus-exposed groups develop IgE, IgG, and IgA anti-Aspergillus antibodies, there is a quantitative increase in IgE anti-Aspergillus antibodies in patients with ABPA.…”

Section: Immunopathogenesis Of Abpa In Cfmentioning

confidence: 97%

Allergic Bronchopulmonary Aspergillosis in Cystic Fibrosis—State of the Art: Cystic Fibrosis Foundation Consensus Conference

et al. 2003

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Because of the difficulties of recognizing allergic bronchopulmonary aspergillosis (ABPA) in the context of cystic fibrosis (because of overlapping clinical, radiographic, microbiologic, and immunologic features), advances in our understanding of the pathogenesis of allergic aspergillosis, new possibilities in therapy, and the need for agreed-upon definitions, an international consensus conference was convened. Areas addressed included fungal biology, immunopathogenesis, insights from animal models, diagnostic criteria, epidemiology, the use of new immunologic and genetic techniques in diagnosis, imaging modalities, pharmacology, and treatment approaches. Evidence from the existing literature was graded, and the consensus views were synthesized into this document and recirculated for affirmation. Virulence factors in Aspergillus that could aggravate these diseases, and particularly immunogenetic factors that could predispose persons to ABPA, were identified. New information has come from transgenic animals and recombinant fungal and host molecules. Diagnostic criteria that could provide a framework for monitoring were adopted, and helpful imaging features were identified. New possibilities in therapy produced plans for managing diverse clinical presentations.

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“…Several fungi, including Aspergillus species, have been implicated in allergic asthma. The symptoms due to mold asthma can be distinguished from those attributable to other allergen-induced asthma by history of exposure, skin test reactivity or in vitro demonstration of Aspergillus -specific IgE in patients’ sera [29, 30]. The lack of reliable fungal extracts poses a problem in the development of standardized in vivo or in vitro tests to diagnose A. fumigatus allergy which needs to be discriminated from ABPA for optimal patient management [31, 32].…”

Section: The Clinical Spectrum Of Aspergillosismentioning

confidence: 99%

Recombinant Aspergillus fumigatus Allergens: From the Nucleotide Sequences to Clinical Applications

Crameri¹

1998

Int Arch Allergy Immunol

197

184

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Members of the genus Aspergillus are opportunistic pathogens for cold- and warm- blooded animals. They are associated with an impressive spectrum of diseases in humans, ranging from benign colonization of the lung to life-threatening diseases such as invasive aspergillosis or allergic bronchopulmonary aspergillosis (ABPA). Aspergillus fumigatus is the etiological agent identified in most of the Aspergillus-related human diseases and is therefore of particular clinical relevance. A major problem in the diagnosis of A. fumigatus-related complications arises from the lack of standardized fungal extracts. The allergenicity of commercial A. fumigatus extracts depends on many factors including the strain used, growth conditions, harvesting and extraction procedures hampering the development of diagnostic reagents suitable for the discrimination between A. fumigatus-related diseases. Molecular DNA/RNA technologies and biotechnological procedures allow cloning, characterization and production of large amounts of single, highly pure allergens. The development of phage surface display technologies for cloning cDNAs has speeded up the isolation of candidate cDNAs encoding allergens. Screening of an A. fumigatus cDNA library displayed on the surface of phage M13 allowed characterization and production of a panel of allergens identified as proteins with known biological function or as allergens without sequence homology to known proteins. They belong to two functional categories: secreted and cytoplasmic proteins. Secreted allergens are recognized by serum IgE of A. fumigatus-sensitized individuals with or without ABPA, whereas nonsecreted allergens are exclusively recognized by serum IgE of ABPA patients. The dissection of IgE-mediated immune responses to single A. fumigatus allergens allows a discrimination between ABPA and A. fumigatus sensitization with high specificity and sensitivity demonstrating the power of recombinant allergens for the differential diagnosis of A. fumigatus-related pulmonary complications.

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Analysis of IgG Subclass and IgE Response in Allergic Disease Caused by <i>Aspergillus fumigatus</i> by Immunoblotting Techniques

Cited by 19 publications

References 20 publications

Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N‐terminal amino acid sequence analysis

Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N‐terminal amino acid sequence analysis

Allergic Bronchopulmonary Aspergillosis in Cystic Fibrosis—State of the Art: Cystic Fibrosis Foundation Consensus Conference

Recombinant Aspergillus fumigatus Allergens: From the Nucleotide Sequences to Clinical Applications

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