Analysis of Long-term Morphological Changes of Micro-patterned Molecules and Cells on PDMS and Glass Surfaces

Abstract

Products were prepared as previously reported. [20][21][22] Detailed procedures for the patterning method and the fabrication method of a mold for patterning, the analysis method, the adsorption of protein, and the cell cultivation can be found in Supporting Information.2017 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. E-mail: yo.tanaka@riken.jp Analysis of Long-term Morphological Changes of Micro-patterned Molecules and Cells on PDMS and Glass SurfacesShun-ichi FUNANO, Nobuyuki TANAKA, and Yo TANAKA † Quantitative Biology Center (QBiC), RIKEN,[1][2][3] Suita, Japan We demonstrated that our previously developed gas-phase fluoroalkylsilane patterning method was applicable to polydimethylsiloxane (PDMS) and we compared the stability of patterned proteins and cultured cells between PDMS and glass surfaces. The shapes of the protein patterns were stable on both glass and PDMS surfaces for more than 1 week. The cell patterns were stable on glass surfaces for 1 week, while those on PDMS collapsed within a few days. These results indicated that our method was applicable to PDMS, although, compared with glass, PDMS has an unsolved issue for its application to long-term patterning of cells.

User‐friendly cell patterning methods using a polydimethylsiloxane mold with microchannels

“…In addition, the cytotoxicity of the fluoro‐functional group was restrictive because the cell pattern was maintained during more than one month of cultivation. Additionally, during cultivation of C2C12 cells, the widths of the cell patterns on the PDMS bottom dishes were measured (Funano, Tanaka, & Tanaka, ). Patterns disappeared by overall cell detachment after the cultivation of 5 days.…”

Flow analysis on microcasting with degassed polydimethylsiloxane micro-channels for cell patterning with cross-linked albumin

“…Furthermore, the gelation process of agarose solution, which requires an uncertain time in the micro-channel, is required in agarose microcasting. The authors have also developed a gas-based cell repellent surface patterning method by using the degassed PDMS to suck a special gas into the microchannel for surface treatment [18,19]. This method could also be used for ultra-small patterning or patterning in a micro-channel [20], which is normally done by a thermal fusion bonding method [21,22].…”

Simple Isolation of Single Cell: Thin Glass Microfluidic Device for Observation of Isolated Single Euglena gracilis Cells

“…Microfluidic devices are considered suitable for overcoming these difficulties because they can achieve precise control of cell manipulation in μL to pL scale and easily fit to a laboratory environment in terms of size, reduced consumption of expensive reagents and requiring fewer number of target cells. 10 These characteristics result in low contamination risk. [11][12][13][14][15] Furthermore, microfluidic devices allow simple operation for manipulating cells, which is highly desired to avoid unintended loss or damage to precious cell samples.…”