Analysis of Matrix-Dependent Cell Migration with a Barrier Migration Assay

Kroening, Sven; Goppelt‐Struebe, Margarete

doi:10.1126/scisignal.3126pl1

Cited by 24 publications

(12 citation statements)

References 13 publications

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“…This limits the potential effect of proliferation even in rapidly dividing cells, such as the breast cancer lines studied. A primary advantage of this microfabrication-based model compared to that of other barrier-type assays 10, 22, 37 was the inclusion of multiple sizes of islands within the same PDMS membrane, which substantially increased the efficiency of the assay. This assay is also highly suitable for cancer migration studies, since it depicted distinct localizations of cancer cells within the islands and an interfacial layer of stromal cells at the periphery, thus providing a more accurate 2D simulation of in vivo mammary tumors.…”

Section: Discussionmentioning

confidence: 99%

Combined Experimental and Mathematical Approach for Development of Microfabrication-Based Cancer Migration Assay

et al. 2011

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Migration of cancer cells is a key determinant of metastasis, which is correlated with poor prognosis in patients. Evidence shows that cancer cell motility is regulated by stromal cell interactions. To quantify the role of homotypic and heterotypic cell-cell interaction on migration, a two-dimensional migration assay has been developed by microfabrication techniques. Two breast cancer cell lines, MDA-MB-231 and MDA-MB-453, were used to develop micropatterns of cancer cells (cell islands) that revealed distinct migration profiles in this assay. Although the individual migration rates of these cells showed only a seven-fold difference, MDA-MB-453 islands migrated significantly lower than MDA-MB-231 islands, indicating differential regulation of migration in isolated cells vs. islands. Island size had the greatest impact on migration, primarily for MDA-MB-231 cells. Migration of MDA-MB-231 islands was decreased by interaction with homotypic cells, and significantly more by heterotypic non-cancer associated fibroblasts. In addition, a mathematical model of island migration in multi-cellular population has been developed using Stefan-Maxwell's equation. The model showed qualitative agreement with experimental results and predicted a biphasic relation between cell densities and island sizes. The combined experimental and mathematical model can be used to quantitatively study the impact of cell-cell interactions on migration.

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Section: Discussionmentioning

confidence: 99%

Combined Experimental and Mathematical Approach for Development of Microfabrication-Based Cancer Migration Assay

et al. 2011

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“…22,24,25 However, excessive force while inserting the stopper can disrupt matrix proteins coated onto the culture surface. To avoid this problem, preinserted stoppers can be purchased in protein-coated plates.…”

Section: Cell Excluding Methodsmentioning

confidence: 99%

Established and novel methods of interrogating two-dimensional cell migration

Ashby

Zijlstra

2012

Integr. Biol.

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The regulation of cell motility is central to living systems. Consequently, cell migration assays are some of the most frequently used in vitro assays. This article provides a comprehensive, detailed review of in vitro cell migration assays both currently in use and possible with existing technology. Emphasis is given to two-dimensional migration assays using densely organized cells such as the scratch assay. Assays are compared and categorized in an outline format according to their primary biological readout and physical parameters. The individual benefits of the various methods and quantification strategies are also discussed. This review provides an in-depth, structured overview of in vitro cell migration assays as a means of enabling the reader to make informed decisions among the growing number of options available for their specific cell migration application.

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“…Human primary tubular epithelial cells (a gift from Sven Kroening, Department of Nephrology and Hypertension, University Hospital of Erlangen) were isolated as described before (Kroening & Goppelt-Struebe, 2010). Cell isolation was performed from tumor-free areas of tumor nephrectomies.…”

Section: Cultivation Of Primary Tubular Epithelial Cellsmentioning

confidence: 99%

Contribution of the α8 Integrin Chain to the Expression of Extracellular Matrix Components

Volkert

Jahn

Dinkel

et al. 2014

Cell Communication & Adhesion

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In the kidney, the α 8 integrin chain (itga8) is expressed in mesenchymal cells and is upregulated in fi brotic disease. We hypothesized that itga8 mediates a profi brotic phenotype of renal cells by promoting extracellular matrix and cytokine expression. Genetic itga8 defi ciency caused complex changes in matrix expression patterns in mesangial and smooth-muscle cells, with the only concordant effect in both cell types being a reduction of collagen III expression. Silencing of itga8 with siRNA led to a decline of matrix turnover with repression of matrix metalloproteinases and reduction of matrix production. In contrast, de novo expression of itga8 in tubular epithelial cells resulted in reduced collagen synthesis. Overexpression of itga8 in fi broblasts did not change the expression of matrix molecules or regulators of matrix turnover. Thus, the infl uence of itga8 on the expression of matrix components was not uniform and celltype dependent. Itga8 seems unlikely to exert overall profi brotic effects in renal cells.

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Analysis of Matrix-Dependent Cell Migration with a Barrier Migration Assay

Cited by 24 publications

References 13 publications

Combined Experimental and Mathematical Approach for Development of Microfabrication-Based Cancer Migration Assay

Combined Experimental and Mathematical Approach for Development of Microfabrication-Based Cancer Migration Assay

Established and novel methods of interrogating two-dimensional cell migration

Contribution of the α8 Integrin Chain to the Expression of Extracellular Matrix Components

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