Analysis of microRNA expression in CD133 positive cancer stem‑like cells of human osteosarcoma cell line MG-63

Xiong, Shunbin; Liu, Weifeng; Liu, Huiqi; Qi, Hui; Wu, Chengai; Ran, Yuliang

doi:10.7717/peerj.12115

Cited by 5 publications

(5 citation statements)

References 47 publications

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“…In the two procedures, known miRNAs were both up-regulated for hsa-miR-493-5p, and down-regulated for hsa-miR-3199 and hsa-miR-184. These miRNAs were mainly active in tumor-related research [ 42 – 44 ]. Nevertheless, in our study, hsa-miR-493-5p was negatively correlated with anthropometric indicators, and glucose metabolism-related indicators, while hsa-miR-184 was positively correlated with WHR, and these results suggested possible objectives of miRNAs that underwent similar changes in SG and RYGB.…”

Section: Discussionmentioning

confidence: 99%

Early changes of microRNAs in blood one month after bariatric surgery

Lu,

Gao,

et al. 2024

Diabetol Metab Syndr

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Background Changes in microRNAs (miRNAs) are relevant to bariatric surgery and its comorbidities. The characteristics of changes in miRNAs of the early postoperative period following both bariatric procedures, sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB), as well as the factors that related to the effectiveness of early weight loss remain unclear. Methods We recruited 18 patients who performed SG and 15 patients who performed RYGB. Their preoperative and 1-month postoperative clinical data and fasting serum samples were collected, and the latter were analyzed by RNA-sequencing. Differential expression analysis of miRNAs was performed by the R-tool. Functional classification annotation and pathway enrichment analysis of targeted genes were analyzed by KOBAS software. The change profiles of miRNAs for both surgeries and their correlation with clinical characteristics and weight loss effectiveness were further analyzed. Results A total of 85 differentially expressed miRNAs were identified before and after SG, while a total of 76 were found before and after RYGB. The target genes of these miRNAs were similar in the Gene Ontology enrichment analysis in SG and RYGB, and the enrichment analysis in the Kyoto Encyclopedia of Genes and Genomes was mainly related to metabolic pathways. Hsa-miR-493-5p, hsa-miR-184, and hsa-miR-3199 exhibited similar changes in SG and RYGB, and the former two were correlated with clinical characteristics. Hsa-miR-6729-5p, hsa-miR-4659b-5p, and hsa-miR-2277-5p were correlated with the weight loss effectiveness of SG, while hsa-miR-4662a-5p was correlated with the weight loss effectiveness of RYGB. Conclusions Short-term metabolic improvement and weight loss occurring after SG and RYGB surgery might be related to changes in miRNAs, which act on multiple biological pathways by regulating genes. In addition, some clinical characteristics and miRNAs were related to the effectiveness of early weight loss after SG and RYGB surgery. Clinical Trial Registration ChiCTR2200058333.

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Section: Discussionmentioning

confidence: 99%

Early changes of microRNAs in blood one month after bariatric surgery

Lu,

Gao,

et al. 2024

Diabetol Metab Syndr

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“…Xiong et al . [ 23 ] found that 6 differentially expressed miRNA target genes in osteosarcoma involved in the PI3K-Akt signaling pathway. Forced expression of ART3 stimulates proliferation of triple-negative breast cancer cells and modulates triple-negative breast carcinogenesis via activation of Akt and ERK pathways [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Zhang et al [22] reported that protein tyrosine kinase is poorly expressed in breast cancer cells and represses invasion and metastasis of breast cancer cells. Xiong et al [23] found that 6 differentially expressed miRNA target genes in osteosarcoma involved in the PI3K-Akt signaling pathway. Forced expression of ART3 stimulates proliferation of triple-negative breast cancer cells and modulates triple-negative breast carcinogenesis via activation of Akt and ERK pathways [24].…”

Section: Discussionmentioning

confidence: 99%

The expression of SERPINE1 in colon cancer and its regulatory network and prognostic value

Wang¹,

Wang²,

Gao³

et al. 2023

BMC Gastroenterol

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Background Serpin Peptidase Inhibitor 1 (SERPINE1) promotes cancer progression by making it easier for cancer cells to spread to surrounding normal tissue. We expect to understand the prognostic value and regulatory network of SERPINE1 in colon cancer using bioinformatics methods. Methods The expression of target gene SERPINE1 in varying cancers was analyzed by the Tumor Immune Estimation Resource (TIMER) database. SERPINE1 expression in Colon Adenocarcinoma and normal tissue samples was assessed by starBase and UALCAN databases. SERPINE1 expression in clinical tissues was assayed using quantitative reverse transcription Polymerase Chain Reaction (qRT-PCR). SERPINE1 expression was detected in colon cancer patients with various clinical features (age, gender, nodal metastasis status, race, stages, and subtype) using analysis of variance. Survival curve was used to analyze the effect of high and low expression of SERPINE1 on the survival time of patients with different clinical phenotypes. Gene Set Enrichment Analysis (GSEA) was conducted on the results of LinkFinder calculation using LinkInterpreter module, which was combined with Pearson correlation analysis to obtain the kinase targets and miRNA targets, transcription factor targets, and corresponding signaling pathways associated with SERPINE1. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed on GSEA result. Finally, Gene Multiple Association Network Integration Algorithm (GeneMANIA) was utilized to establish a network of genes related to the kinases MAPK1, miR-18a, and SRF_Q, and biological functions were analyzed. Results Based on TIMER, starBase, and UALCAN databases, SERPINE1 was found to be remarkably highly expressed in colon cancer patients, which was further verified by clinical tissue. It was also associated with different clinical features (nodal metastasis status, stages, subtypes). Additionally, survival analysis showed that patients with low expression of SERPINE1 had a longer survival time, suggesting that SERPINE1 was a prognostic risk factor for colon cancer. Pearson correlation analysis revealed that the expression of Integrin Alpha 5 (ITGA5), Matrix Metallopeptidase 19 (MMP19), and ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4) had the highest correlation with that of SERPINE1. The GSEA results indicated that these genes were mainly enriched in the pathways of RNA expression and kinases. Finally, GeneMANIA analysis was introduced to construct the molecular network of SERPINE1. Conclusion Overall, our bioinformatics analyses comprehensively described the networks involved SERPINE1 in colon cancer and the potentially associated molecular mechanisms.

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“…To the best of our knowledge, miRNA-487b has not been described in renal cancer. However, miRNA-487b was shown to be downregulated in osteosarcoma CD133 positive cell lines [ 40 ] and inhibited osteosarcoma chemoresistance and metastasis [ 41 ]. The suppressive role of miRNA-487b was also indicated in colorectal cancer via targeting MYC, SUZ12 and KRAS [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Signatures in Clear Cell Renal Cell Carcinoma: High-Throughput Searching for Key miRNA Markers in Patients from the Volga-Ural Region of Eurasian Continent

Gilyazova

Ivanova

Измайлов

et al. 2023

IJMS

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Clear cell renal cell carcinoma (ccRCC) is characterized by high molecular genetic heterogeneity, metastatic activity and unfavorable prognosis. MicroRNAs (miRNA) are 22-nucleotide noncoding RNAs that are aberrantly expressed in cancer cells and have gained serious consideration as non-invasive cancer biomarkers. We investigated possible differential miRNA signatures that may differentiate high-grade ccRCC from primary disease stages. High-throughput miRNAs expression profiling, using TaqMan OpenArray Human MicroRNA panel, was performed in a group of 21 ccRCC patients. The obtained data was validated in 47 ccRCC patients. We identified nine dysregulated miRNAs (miRNA-210, -642, -18a, -483-5p, -455-3p, -487b, -582-3p, -199b and -200c) in tumor ccRCC tissue compared to normal renal parenchyma. Our results show that the combination of miRNA-210, miRNA-483-5p, miRNA-455 and miRNA-200c is able to distinguish low and high TNM ccRCC stages. Additionally, miRNA-18a, -210, -483-5p and -642 showed statistically significant differences between the low stage tumor ccRCC tissue and normal renal tissue. Contrariwise, the high stages of the tumor process were accompanied by alteration in the expression levels of miRNA-200c, -455-3p and -582-3p. Although the biological roles of these miRNAs in ccRCC are not totally unclear, our findings need additional investigations into their involvement in the pathogenesis of ccRCC. Prospective studies with large study cohorts of ccRCC patients are important to further establish the clinical validity of our miRNA markers to predict ccRCC.

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Analysis of microRNA expression in CD133 positive cancer stem‑like cells of human osteosarcoma cell line MG-63

Cited by 5 publications

References 47 publications

Early changes of microRNAs in blood one month after bariatric surgery

Early changes of microRNAs in blood one month after bariatric surgery

The expression of SERPINE1 in colon cancer and its regulatory network and prognostic value

MicroRNA Expression Signatures in Clear Cell Renal Cell Carcinoma: High-Throughput Searching for Key miRNA Markers in Patients from the Volga-Ural Region of Eurasian Continent

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