Analysis of mitochondrial oxygen consumption and hydrogen peroxide release from cardiac mitochondria using electrochemical multi-sensors

Cheng, Ming-Hao; Chicco, Adam J.; Ball, Daniel R.; Chen, Thomas W.

doi:10.1016/j.snb.2022.131641

Cited by 14 publications

(7 citation statements)

References 21 publications

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“…Assays of single oocyte metabolic function were performed using a microchamber with electrochemical-based oxygen and pH sensors. Fabrication of the electrochemical sensor chips and hardware in the device used in this study were previously reported 27 , 96 . The microchamber was filled with 180 μL of MOPS-buffered medium (G-MOPS™) and placed inside an incubator at 38.5 °C 96 .…”

Section: Methodsmentioning

confidence: 99%

“…Fabrication of the electrochemical sensor chips and hardware in the device used in this study were previously reported 27 , 96 . The microchamber was filled with 180 μL of MOPS-buffered medium (G-MOPS™) and placed inside an incubator at 38.5 °C 96 . Approximately 15 min before the assay, each denuded oocyte was warmed to 38.5 °C and pipetted onto the working electrode of the oxygen sensor, after oxygen and pH sensors had reached a steady baseline state.…”

Section: Methodsmentioning

confidence: 99%

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Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Catandi,

Fresa,

Cheng

et al. 2024

Sci Rep

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Obesity is a growing concern in human and equine populations, predisposing to metabolic pathologies and reproductive disturbances. Cellular lipid accumulation and mitochondrial dysfunction play an important role in the pathologic consequences of obesity, which may be mitigated by dietary interventions targeting these processes. We hypothesized that obesity in the mare promotes follicular lipid accumulation and altered mitochondrial function of oocytes and granulosa cells, potentially contributing to impaired fertility in this population. We also predicted that these effects could be mitigated by dietary supplementation with a combination of targeted nutrients to improve follicular cell metabolism. Twenty mares were grouped as: Normal Weight [NW, n = 6, body condition score (BCS) 5.7 ± 0.3], Obese (OB, n = 7, BCS 7.7 ± 0.2), and Obese Diet Supplemented (OBD, n = 7, BCS 7.7 ± 0.2), and fed specific feed regimens for ≥ 6 weeks before sampling. Granulosa cells, follicular fluid, and cumulus-oocyte complexes were collected from follicles ≥ 35 mm during estrus and after induction of maturation. Obesity promoted several mitochondrial metabolic disturbances in granulosa cells, reduced L-carnitine availability in the follicle, promoted lipid accumulation in cumulus cells and oocytes, and increased basal oocyte metabolism. Diet supplementation of a complex nutrient mixture mitigated most of the metabolic changes in the follicles of obese mares, resulting in parameters similar to NW mares. In conclusion, obesity disturbs the equine ovarian follicle by promoting lipid accumulation and altering mitochondrial function. These effects may be partially mitigated with targeted nutritional intervention, thereby potentially improving fertility outcomes in the obese female.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Catandi,

Fresa,

Cheng

et al. 2024

Sci Rep

Self Cite

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“…Mitochondria play an important role in hypoxia/reoxygenation injury because they consume over 95% of all oxygen that reaches our cells in order to produce ATP through oxidative phosphorylation [ 8 , 9 ]. They are also known to produce large amounts of reactive oxygen species (ROS) during hypoxia/reoxygenation, which also amplifies the damage by inducing the nonspecific oxidation of mitochondrial and cellular proteins, DNA, and various phospholipids, especially cardiolipin [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Hypoxia/Reoxygenation Induces Mitochondrial Cardiolipin Remodeling in Human Kidney Cells

Strazdauskas,

Trumbeckaite,

Jakstas

et al. 2024

IJMS

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Renal ischemia/reperfusion is a serious condition that not only causes acute kidney injury, a severe clinical syndrome with high mortality, but is also an inevitable part of kidney transplantation or other kidney surgeries. Alterations of oxygen levels during ischemia/reperfusion, namely hypoxia/reoxygenation, disrupt mitochondrial metabolism and induce structural changes that lead to cell death. A signature mitochondrial phospholipid, cardiolipin, with many vital roles in mitochondrial homeostasis, is one of the key players in hypoxia/reoxygenation-induced mitochondrial damage. In this study, we analyze the effect of hypoxia/reoxygenation on human renal proximal tubule epithelial cell (RPTEC) cardiolipins, as well as their metabolism and mitochondrial functions. RPTEC cells were placed in a hypoxic chamber with a 2% oxygen atmosphere for 24 h to induce hypoxia; then, they were replaced back into regular growth conditions for 24 h of reoxygenation. Surprisingly, after 24 h, hypoxia cardiolipin levels substantially increased and remained higher than control levels after 24 h of reoxygenation. This was explained by significantly elevated levels of cardiolipin synthase and lysocardiolipin acyltransferase 1 (LCLAT1) gene expression and protein levels. Meanwhile, hypoxia/reoxygenation decreased ADP-dependent mitochondrial respiration rates and oxidative phosphorylation capacity and increased reactive oxygen species generation. Our findings suggest that hypoxia/reoxygenation induces cardiolipin remodeling in response to reduced mitochondrial oxidative phosphorylation in a way that protects mitochondrial function.

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“…Even though enzyme-based electrochemistry has high selectivity and sensitivity, the difficulty in immobilizing and stabilizing enzymes limits their use. 11–13…”

Section: Introductionmentioning

confidence: 99%

“…Even though enzyme-based electrochemistry has high selectivity and sensitivity, the difficulty in immobilizing and stabilizing enzymes limits their use. [11][12][13] Jeong-Hyeop et al suggested a biosensor that works by the electrocatalytic activity of horseradish peroxidase (HRP)encapsulated protein nanoparticles (HEPNP) for the detection of H 2 O 2 . 14 Ali-saad Elewi et al also developed a biosensor based on hemoglobin immobilized on a screen-printed carbon electrode (SPCE).…”

Section: Introductionmentioning

confidence: 99%

A smartphone-interfaced, low-cost colorimetry biosensor for selective detection of bronchiectasisviaan artificial neural network

et al. 2022

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Analysis of mitochondrial oxygen consumption and hydrogen peroxide release from cardiac mitochondria using electrochemical multi-sensors

Cited by 14 publications

References 21 publications

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

In Vitro Hypoxia/Reoxygenation Induces Mitochondrial Cardiolipin Remodeling in Human Kidney Cells

A smartphone-interfaced, low-cost colorimetry biosensor for selective detection of bronchiectasisviaan artificial neural network

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