Analysis of mRNA Expression Patterns in Peripheral Blood Cells of 3 Patients With Cancer After the First Fraction of 2 Gy Irradiation: An Integrated Case Report and Systematic Review

Abstract

Background: Radiation therapy induces acute and chronic radiological toxicity, in particular hematological toxicity (HT). This study aimed to explore the mechanistic clue and potential predictors at the messenger RNA (mRNA) level. Materials and Methods: Peripheral blood was collected from 3 patients with cervical cancer (CC), nasopharynx cancer (NC), and tongue cancer (TC) after the first 2 Gy fraction of radiotherapy (RT). High-throughput sequencing was used to assess mRNA profiles. Results: Eleven genes, such as ALAS2(5-aminolevulinate synthase), SLC4A1(solute carrier family 4 member 1), HBG2 (hemoglobin subunit gamma 2), TNFAIP3 (TNF α-induced protein 3), PER1 (period circadian clock 1), CCDC136 (coiled-coil domain containing 136), C9orf84 (chromosome 9 open reading frame 84), IL1B (interleukin 1β), FOSB (FosB protooncogene), NR4A2 (nuclear receptor subfamily 4), PARP15 (polymerase family member 15), had overlapping expression changes in all 3 cancers of which 3 ( ALAS2 , FOSB, and HBG2 ) are suggested as potential predictors for the early diagnosis of HT after RT. Conclusions: ALAS2, FOSB, and HBG2 may be useful predictors of HT in patients after RT. Eleven overlapping expression mRNAs among 3 cancers might be potential predictors for early diagnosis of radiation toxicity in patients.

Analysis of lncRNA-miRNA-mRNA Expression Pattern in Heart Tissue After Total Body Irradiation in a Mouse Model

“…Alas2, the rate limiting enzyme in heme synthesis, became increasingly downregulated as radiation dose increased. Similar downregulation of Alas2 was recently cited as a potential predictive marker for radiation induced hematological toxicity in cancer patients [74]. Other genes associated with hemoglobin synthesis, including Hbb-bt, Hbb-b1, and Hba-a1, were also downregulated.…”

Gene signature based on B cell predicts clinical outcome of radiotherapy and immunotherapy for patients with lung adenocarcinoma

“…PARP15, polymerase family member 15, was originally confirmed as a risk-related gene in diffuse large B cell lymphomas, and might be potential predictors of hematological toxicity associated with RT for acute myeloid leukemia, cervical cancer, nasopharynx cancer, and tongue cancer. [36][37][38][39][40] FADS3, as a fatty acid desaturase, was located along with FADS1 and FADS2, 41 which were reported to be associated with the occurrence and development of NSCLC and colon cancer. 42,43 Promoter methylation of RUBCNL was identified as a potential biomarker for early diagnosis of cervical cancer.…”

“…In the TILBSig, 3 B cell‐specific genes (PARP15, FADS3, and RUBCNL) were correlated with better prognosis, while the other three (BIRC3, SP110, and TLE1) were correlated with poor survival. PARP15, polymerase family member 15, was originally confirmed as a risk‐related gene in diffuse large B cell lymphomas, and might be potential predictors of hematological toxicity associated with RT for acute myeloid leukemia, cervical cancer, nasopharynx cancer, and tongue cancer 36–40 . FADS3, as a fatty acid desaturase, was located along with FADS1 and FADS2, 41 which were reported to be associated with the occurrence and development of NSCLC and colon cancer 42,43 .…”

Integrated analysis of transcriptomic and metabolomic profiling reveal the p53 associated pathways underlying the response to ionizing radiation in HBE cells

“…On one hand, IR has most susceptible to IR-induced injury. In our laboratory, we noted that, after the initial treatment of cancer patients with 2 Gy radiation, multiple mRNAs changed significantly compared to their levels prior to radiation treatment, with most of the affected mRNAs, such as ALAS2 and HBG2, showing the effects of hematological toxicity [10]. In fact, while radiation injury generally depends on the dose, exposure duration, and type of radiation, as well as on the gender, age and health status of the exposed individual, the underlying molecular mechanisms are related to lipid, protein and DNA damage caused by direct ionization of target molecules, and to the effects of hydroxyl radicals formed via radiolysis of water molecules [11].…”