Analysis of Multi-Sample Pools in the Detection of SARS-CoV-2 RNA for Mass Screening: An Indian Perspective

Deka, Sangeeta; Kalita, Deepjyoti; Mangla, Amit; Shankar, Ravi

doi:10.4103/ijmm.ijmm_20_273

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…Mass screening, in turn, is limited by access of the population to laboratory testing, which in the case of PCR is limited by the capabilities of laboratories. In this regard, many states are adopting new approaches, whereby samples are pooled to increase the amount of PCR tests [15], [16], [17], [18].…”

Section: Discussionmentioning

confidence: 99%

The Value of Rapid Antigen Tests to Identify Carriers of Viable SARS-CoV-2

Shidlovskaya

Kuznetsova

Divisenko

et al. 2021

Preprint

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The search for effective methods to detect patients who excrete a viable virus is one of the urgent tasks of modern biomedicine. In the present study, we examined the diagnostic value of two antigen tests BIOCREDIT COVID-19 Ag (RapiGEN Inc., Korea) and SGTI-flex COVID-19 Ag (Sugentech Inc., Korea) for their diagnostic value in identifying patients who excrete viable SARS-CoV-2. As part of the study, we examined samples from 106 patients who had just been admitted to the hospital, who had undergone quantitative RT-PCR and assessment of viability of SARS-CoV-2 using cell culture. Sensitivity was 0.786 (0.492-0.953) for SGTI-flex COVID-19 Ag and 1 (0.768-1) for Biocredit COVID-19 Ag. Specificity of rapid tests was significantly higher than that of RT-PCR and was 0.663 (0.557-0.758) and 0.674 (0.568-0.768) for SGTI-flex COVID-19 Ag and Biocredit COVID-19 Ag versus 0.304 (0.213-0.409) obtained for PCR. Thus, for tasks of identifying viable SARS-CoV-2 during screening of conditionally healthy people, as well as monitoring those quarantined, rapid tests show significantly better results.

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Section: Discussionmentioning

confidence: 99%

The Value of Rapid Antigen Tests to Identify Carriers of Viable SARS-CoV-2

Shidlovskaya

Kuznetsova

Divisenko

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Mass screening, in turn, is limited by the access of the population to laboratory testing, which in the case of PCR is limited by the capabilities of laboratories. In this regard, many states are adopting new approaches, whereby samples are pooled to increase the amount of PCR tests [ 8 , 9 , 10 , 11 ]. An alternative to using PCR laboratories is to use rapid antigen tests, including for home use.…”

Section: Discussionmentioning

confidence: 99%

The Value of Rapid Antigen Tests for Identifying Carriers of Viable SARS-CoV-2

Shidlovskaya

Kuznetsova

Divisenko

et al. 2021

Viruses

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The search for effective methods to detect patients who excrete a viable virus is one of the urgent tasks of modern biomedicine. In the present study, we examined the diagnostic value of two antigen tests, BIOCREDIT COVID-19 Ag (RapiGEN Inc., Anyang, Korea) and SGTI-flex COVID-19 Ag (Sugentech Inc., Cheongju, Korea), for their diagnostic value in identifying patients who excrete viable SARS-CoV-2. As part of the study, we examined samples from 106 patients who had just been admitted to the hospital and who had undergone quantitative RT-PCR and assessment of viability of SARS-CoV-2 using cell culture. Assessment of the tests’ value for detecting samples containing viable virus showed high sensitivity for both tests. Sensitivity was 78.6% (95% CI, from 49.2% to 95.3%) for SGTI-flex COVID-19 Ag and 100% (95% CI, from 76.8% to 100%) for Biocredit COVID-19 Ag. The specificity of rapid tests was significantly higher than that of RT-PCR and was 66.3% (95% CI, from 55.7% to 75.8%) and 67.4% (95% CI, from 56.8% to 76.8%) for SGTI-flex COVID-19 Ag and Biocredit COVID-19 Ag versus 30.4% (95% CI, from 21.3% to 40.9%) obtained for PCR. Thus, for tasks of identifying viable SARS-CoV-2 during screening of conditionally healthy people, as well as monitoring those quarantined, rapid tests show significantly better results.

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Pooled Testing Strategies for SARS-CoV-2 diagnosis: A comprehensive review

Daniel

Anbalagan

et al. 2021

Diagnostic Microbiology and Infectious Disease

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SARS-CoV-2 has surged across the globe causing the ongoing COVID-19 pandemic. Systematic testing to facilitate index case isolation and contact tracing is needed for efficient containment of viral spread. The major bottleneck in leveraging testing capacity has been the lack of diagnostic resources. Pooled testing is a potential approach that could reduce cost and usage of test kits. This method involves pooling individual samples and testing them ‘ en bloc ’. Only if the pool tests positive, retesting of individual samples is performed. Upon reviewing recent articles on this strategy employed in various SARS-CoV-2 testing scenarios, we found substantial diversity emphasizing the requirement of a common protocol. In this article, we review various theoretically simulated and clinically validated pooled testing models and propose practical guidelines on applying this strategy for large scale screening. If implemented properly, the proposed approach could contribute to proper utilization of testing resources and flattening of infection curve.

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Analysis of Multi-Sample Pools in the Detection of SARS-CoV-2 RNA for Mass Screening: An Indian Perspective

Cited by 4 publications

References 6 publications

The Value of Rapid Antigen Tests to Identify Carriers of Viable SARS-CoV-2

The Value of Rapid Antigen Tests to Identify Carriers of Viable SARS-CoV-2

The Value of Rapid Antigen Tests for Identifying Carriers of Viable SARS-CoV-2

Pooled Testing Strategies for SARS-CoV-2 diagnosis: A comprehensive review

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