Analysis of nucleotides directly from TLC plates using MALDI-MS detection

Isbell, Donna T.; Gusev, Arkady I.; Taranenko, N. I.; Chen, C. H.; Hercules, David M.

doi:10.1007/s002160051534

Cited by 23 publications

(20 citation statements)

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“…Many classes of compounds have so far been analyzed by TLC with direct on-plate (MA)LDI-MS detection, including dyes (Gusev et al, 1995a), products of various organic reactions (St Hilaire et al, 1998), metal complexes (Matsumoto et al, 2002), siderophores from bacterial samples (Hayen & Volmer, 2005), pharmaceuticals and their impurities (Mowthorpe et al, 1999;Crecelius et al, 2002Crecelius, Clench, & Richards, 2004a;Salo et al, 2007), peptides (Gusev et al, 1995a;Mehl & Hercules, 2000), proteins (Gusev et al, 1995b;Bakry et al, 2007), nucleotides (Isbell et al, 1999), polymers (Matsumoto et al, 1999), as well as gangliosides (Ivleva et al, 2004(Ivleva et al, , 2005Dreisewerd et al, 2005), glycosphingolipids (GSLs) (Guittard, Hronowski, & Costello, 1999;Distler et al, 2008), LAB-ON-A-PLATE & phospholipids (Fuchs et al, 2007;Rohlfing et al, 2007), stem cell lipids (Fuchs et al, 2008), and bacterial toxins (Meisen et al, 2009). An example is shown in Figure 20.…”

Section: On-plate Separationmentioning

confidence: 99%

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Urban

Amantonico

Zenobi

2011

Mass Spectrometry Reviews

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We review the literature that describes how (matrix-assisted) laser desorption/ionization (MA)LDI target plates can be used not only as sample supports, but beyond that: as functional parts of analytical protocols that incorporate detection by MALDI-MS or matrix-free LDI-MS. Numerous steps of analytical procedures can be performed directly on the (MA)LDI target plates prior to the ionization of analytes in the ion source of a mass spectrometer. These include homogenization, preconcentration, amplification, purification, extraction, digestion, derivatization, synthesis, separation, detection with complementary techniques, data storage, or other steps. Therefore, we consider it helpful to define the "lab-on-a-plate" as a format for carrying out extensive sample treatment as well as bioassays directly on (MA)LDI target plates. This review introduces the lab-on-plate approach and illustrates it with the aid of relevant examples from the scientific and patent literature.

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Section: On-plate Separationmentioning

confidence: 99%

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Urban

Amantonico

Zenobi

2011

Mass Spectrometry Reviews

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“…TLC separation of larger peptides with detection by MALDI-TOF-MS was described as well (Bakry et al 2007). The detection of smaller oligonucleotides is possible by using 2,4,6-trihydroxyacetophone as matrix and ammonium hydroxide/methanol as solvent system, and then the detection limits in the range 10 pg were reported (Isbell et al 1999). In the next example, capabilities and disadvantages of combined MALDI-TOF MS and HPTLC for analysis of egg yolk lipids was described (Fuchs et al 2009).…”

Section: Thin Layer Chromatography/mass Spectrometry (Tlc-ms)mentioning

confidence: 99%

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

Tuzimski

2010

High-Performance Thin-Layer Chromatography (HPTLC)

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Sample preparation, detection, identification, and quantitative determination of biomolecules are presented in this chapter. Advantages of planar chromatography and the basic principles (chambers, sample application, and chromatogram development) are also described. Rapid detection of biomolecules plays a strategical role in their investigation. Hyphenated techniques such as planar chromatography coupled to UV diode array detection and to mass spectrometry provide on-line extensive structural information on the metabolites prior to their isolation. In this chapter, the combination of thin-layer chromatography (TLC) with biomolecules specific detection by diode array scanning (DAD), mass spectrometry (MS), nuclear magnetic resonance (NMR), and Fourier transform infrared spectroscopy (TLC-FTIR) is discussed. In the last part of the chapter, the reader will gain useful information about a recent method of planar chromatography -multidimensional planar chromatography (MD-PC) and information on application of different modes of multidimensional planar chromatography and combination of this technique with diode array detection (MD-PC-DAD) and HPLC-DAD for separation, detection, and qualitative and quantitative determination of biomolecules. Planar chromatography as a pilot technique for HPLC is also described.Biomolecules are widespread throughout the world. It is an organic molecule produced by a living organism, including large polymeric molecules such as proteins, polysaccharides, and nucleic acids as well as small molecules such as a primary metabolites, secondary metabolites, and natural products. Many sample-preparation techniques are used in biomolecule analysis; the method selected depends on the

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“…Nevertheless, the detection of smaller oligonucleotides (obtained by enzymatic digestion of native DNA prior to TLC) is possible without major problems (Isbell et al 1999). Using 2,4,6-trihydroxyacetophenone as matrix and ammonium hydroxide/ methanol as solvent system, detection limits of small oligonucleotides in the range of about 10 pg were reported (Isbell et al 1999).…”

Section: Dna and Nucleic Acidsmentioning

confidence: 99%

“…Using 2,4,6-trihydroxyacetophenone as matrix and ammonium hydroxide/ methanol as solvent system, detection limits of small oligonucleotides in the range of about 10 pg were reported (Isbell et al 1999). …”

Section: Dna and Nucleic Acidsmentioning

confidence: 99%

TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years

Schiller

Fuchs

Teuber

et al. 2010

High-Performance Thin-Layer Chromatography (HPTLC)

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Thin-layer chromatography (TLC) is a widely used, fast and inexpensive method. Even minor constituents of complex mixtures can be often characterized. Unfortunately, the unambiguous assignment of the spots to defined compounds is often difficult because common staining methods normally reveal only (at best) a substance class, not a specific compound. For instance, in the case of phospholipids, the lipid class, but not the detailed fatty acyl composition, can be determined (normal phase TLC). Nowadays mass spectrometry (MS) seems to be the most suitable method for analyte characterization due to its high sensitivity and mass accuracy. This became possible by the invention of soft ionization methods such as matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) MS and electrospray ionization (ESI) although many further desorption techniques are available nowadays. This review summarizes the so far available knowledge about direct TLC/MS couplings and gives an overview about selected compounds that could be successfully analyzed. Chromatographic Methods and Product IdentificationLiquid chromatographic methods, especially high-performance liquid chromatography (HPLC) and planar chromatography [in particular thin-layer (TLC) or highperformance thin-layer chromatography (HPTLC)], are indispensable tools of modern analytical chemistry (Hahn-Deinstrop 2006 l TLC is a very robust system and to a much lesser extent affected by impurities potentially present in the sample. Thus, TLC may also be used for the analysis of "suspicious" samples (for instance, crude tissue or food extracts) that might easily damage or plug the column of an HPLC system. l HPLC columns are normally rather expensive and are, thus, many times reused subsequent to careful washing to remove previous analytes. However, it can never be completely ruled out that there is some residual material on the column. Thus, it is often more difficult to certify an HPLC assay than a TLC assay because TLC does not provide any "memory" effects as a completely new stationary phase is used in all cases. The consumption of solvents is much lower than in the case of HPLC. This makes TLC less expensive and particularly less harmful and more environment friendly.l Although nowadays there are also "multiplexing" HPLC systems available (Woodbury et al. 1995) that allow the simultaneous analysis of several samples in parallel, the simple analysis of a multitude of samples is also a clear advantage of TLC.There is hardly a class of small molecules that have not yet been analyzed by TLC, and thus this review will not deal with improvements of the achievable separation quality, neither by optimizing the stationary phase nor the solvent system nor sample application.However, separation is only one (unequivocally very important) step of successful analysis, and the detection of the different analytes on the TLC plate and their structural elucidation is also very important. This particularly holds because the retardation factor (R f value) by which a com...

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Analysis of nucleotides directly from TLC plates using MALDI-MS detection

Cited by 23 publications

References 0 publications

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years

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