Analysis of outer membrane vesicle protein involved in biofilm formation of Helicobacter pylori

Yonezawa, Hideo; Osaki, Takako; Woo, Timothy; Kurata, Satoru; Zaman, Cynthia; Hojo, Fuhito; Hanawa, Tomoko; Kato, Shigeaki; Kamiya, Shigeru

doi:10.1016/j.anaerobe.2011.03.020

Cited by 74 publications

(51 citation statements)

References 22 publications

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“…Temperature is a major regulator of gene expression in Y. pestis , and OMV production by other bacteria has been observed at both low and higher temperatures [7], [8], [44]. With this in mind, we examined whether changes in temperature affect OMV production by Y. pestis .…”

Section: Resultsmentioning

confidence: 99%

Production of Outer Membrane Vesicles by the Plague Pathogen Yersinia pestis

et al. 2014

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Many Gram-negative bacteria produce outer membrane vesicles (OMVs) during cell growth and division, and some bacterial pathogens deliver virulence factors to the host via the release of OMVs during infection. Here we show that Yersinia pestis, the causative agent of the disease plague, produces and releases native OMVs under physiological conditions. These OMVs, approximately 100 nm in diameter, contain multiple virulence-associated outer membrane proteins including the adhesin Ail, the F1 outer fimbrial antigen, and the protease Pla. We found that OMVs released by Y. pestis contain catalytically active Pla that is competent for plasminogen activation and α2-antiplasmin degradation. The abundance of OMV-associated proteins released by Y. pestis is significantly elevated at 37°C compared to 26°C and is increased in response to membrane stress and mutations in RseA, Hfq, and the major Braun lipoprotein (Lpp). In addition, we show that Y. pestis OMVs are able to bind to components of the extracellular matrix such as fibronectin and laminin. These data suggest that Y. pestis may produce OMVs during mammalian infection and we propose that dispersal of Pla via OMV release may influence the outcome of infection through interactions with Pla substrates such as plasminogen and Fas ligand.

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Section: Resultsmentioning

confidence: 99%

Production of Outer Membrane Vesicles by the Plague Pathogen Yersinia pestis

et al. 2014

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“…For Myxococcus xanthus , for instance, in addition to the predatory aspects of its OMVs (see main text), this organism increases OMV production as well as the formation of outer-membrane extensions that seem to interconnect cells during the biofilm stage as compared with growth in aqueous culture 96 . For Helicobacter pylori , a unique 22 kDa protein of unknown function was present in OMVs in early biofilms but disappeared during biofilm maturation, suggesting a role for this OMV protein as a novel colonization factor 127,128 . Extracellular genomic DNA was determined to be associated with Francisella spp.…”

Section: Figurementioning

confidence: 99%

Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions

2015

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Outer-membrane vesicles (OMVs) are spherical buds of the outer membrane filled with periplasmic content and are commonly produced by Gram-negative bacteria. The production of OMVs allows bacteria to interact with their environment, and OMVs have been found to mediate diverse functions, including promoting pathogenesis, enabling bacterial survival during stress conditions and regulating microbial interactions within bacterial communities. Additionally, because of this functional versatility, researchers have begun to explore OMVs as a platform for bioengineering applications. In this Review, we discuss recent advances in the study of OMVs, focusing on new insights into the mechanisms of biogenesis and the functions of these vesicles.

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“…OMV production in H. pylori , similar to that observed in other bacteria, depends on culture conditions and the physiological state of the cells, as more OMV production and enhanced biofilm formation were observed in growth medium containing fetal calf serum in a dose-dependent manner (107). Although the protein compositions of OMVs produced by H. pylori have not been determined, protein profiles, as determined by SDS-PAGE, indicate that OMVs isolated at different stages of biofilm formation exhibit differences (111), suggesting that these OMVs may have distinct cargo.…”

Section: Outer Membrane Vesicles (Omvs)mentioning

confidence: 99%

Biofilm Matrix Proteins

2015

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Proteinaceous components of the biofilm matrix include secreted extracellular proteins, cell surface adhesins and protein subunits of cell appendages such as flagella and pili. Biofilm matrix proteins play diverse roles in biofilm formation and dissolution. They are involved in attaching cells to surfaces, stabilizing the biofilm matrix via interactions with exopolysaccharide and nucleic acid components, developing three-dimensional biofilm architectures, and dissolving biofilm matrix via enzymatic degradation of polysaccharides, proteins, and nucleic acids. In this chapter, we will review functions of matrix proteins in a selected set of microorganisms, studies of the matrix proteomes of Vibrio cholerae and Pseudomonas aeruginosa, and roles of outer membrane vesicles and of nucleoid-binding proteins in biofilm formation.

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Analysis of outer membrane vesicle protein involved in biofilm formation of Helicobacter pylori

Cited by 74 publications

References 22 publications

Production of Outer Membrane Vesicles by the Plague Pathogen Yersinia pestis

Production of Outer Membrane Vesicles by the Plague Pathogen Yersinia pestis

Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions

Biofilm Matrix Proteins

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