Analysis of phenolic compounds and antioxidant activity with H4IIE cells of three different rice grain varieties

Chi, Hee-Youn; Lee, Chang-Ho; Kim, Kwang‐Ho; Kim, Sun-Lim; Chung, Ill‐Min

doi:10.1007/s00217-006-0498-3

Cited by 24 publications

(26 citation statements)

References 20 publications

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“…The correlation and regression analysis showed that the TPC had a significant positive correlation with antioxidant activity of the rice suggesting that they may be responsible for the antioxidant activity seen in the rice varieties. Similar results were also seen in some earlier studies (Chi et al 2007;Jin et al 2009). …”

Section: Correlation Analysissupporting

confidence: 92%

Study of chemical composition and volatile compounds along with in-vitro assay of antioxidant activity of two medicinal rice varieties: Karungkuravai and Mappilai samba

et al. 2014

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The objective of this study is to analyze the chemical composition and volatiles present in two medicinal rice varieties of "karungkuravai" and "mappilai samba" and to investigate their total phenolic content and bio activity. Chemical composition of the rice varieties was analyzed using gas chromatography/mass spectroscopy (GC-MS), the volatile compounds are identified using static head space analysis (SHS) followed by GC-MS. Total phenolic content (TPC) is estimated using Folin Ciocalteu colorimetric method, antioxidant assay is done using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay and ferric reducing antioxidant power (FRAP) assay. GC-MS and SHS analysis identified pharmaceutically important phytochemicals present in the rice variety. The compounds like "curlone" and "7-chloro-1,3-dihydro-5-phenyl-1-(trimethylsilyl)-2H-1-4-benzodiazapene-2-one" are identified for the first time in any rice variety. Phenols are found to be present in both rice varieties. Both rice varieties also showed antioxidant activity in both DPPH and FRAP assays and the IC 50 values were 91.08 μg/ml and 359.93 μg/ml for "karungkuravai" and "mappilai samba", respectively. . The correlation coefficient and regression analysis of total phenolic content with DPPH assay and FRAP assay show significant positive correlation coefficient values and coefficient of regression values.

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Section: Correlation Analysissupporting

confidence: 92%

Study of chemical composition and volatile compounds along with in-vitro assay of antioxidant activity of two medicinal rice varieties: Karungkuravai and Mappilai samba

et al. 2014

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“…To understand the full variation in phytochemicals in the evaluated rice cultivars, more sophisticated and expensive HPLC methods are a requirement. Our study confirmed the existence of a strong positive relation between phenolic compounds and antioxidant capacity, also reported by other researches [56,57]. Bergman and Goffman [58] argued that phenolic compounds are the main factors of the antioxidant activity of rice grains.…”

Section: 2supporting

confidence: 92%

Quality and Grain Yield Attributes of Rwandan Rice (Oryza sativaL.) Cultivars Grown in a Biotron Applying Two NPK Levels

Mukamuhirwa

Hovmalm

Ortíz

et al. 2018

Journal of Food Quality

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High-yielding rice cultivars with good processing quality and rich in nutrition suitable to a changing climate are of particular importance for future rice-based food production. Here, seven Rwandan rice cultivars were grown in a climate chamber of the biotron facility at the Swedish University of Agricultural Sciences, to be evaluated for their grain yield, nutritional composition, and dough mixing properties. Two di erent levels of inorganic fertilizer were applied weekly from the seedling stage until owering. Signi cant di erences for grain yield and quality attributes were found between cultivars. Jyambere showed signi cantly the highest yield while Ingwizabukungu, Nemeyubutaka, and Jyambere were high in mineral elements content. Ndamirabahinzi and Mpembuke had the highest levels of TPC and TAC. Generally, the lower fertilizer dose resulted in a better performance of the cultivars for both yield and quality attributes. Signi cantly higher content of Fe, Ca, and Ba was found in grains from the moderate fertilizer dose, whereas K, Na, P, S, Zn, Cd, and Pb increased in grains from the higher fertilizer dose. e cultivar Ndamirabahinzi showed less variability of evaluated characters across fertilizer doses. e results from this study may be used for rice breeding of cultivars with high yield and good grain quality.

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“…After incubation, H4IIE cells were treated with peptide fractions of chungkukjang in the presence and absence of 250 μM H2O2-induced oxidative stress. The antioxidant activity was evaluated by measuring the intensity of dichlorofluorescein (DCF) fluorescence using fluorescence-activated cell sorting (FACS, Becton Dickinson, USA) analysis (Chi et al, 2007).…”

Section: Assessment Of Cell Viability and Dcfh-da Oxidationmentioning

confidence: 99%

Evaluation of Antioxidant Activities of Peptides Isolated from Korean Fermented Soybean Paste, Chungkukjang

Kim¹,

Chi²,

Kim³

et al. 2011

Korean Journal of Crop Science

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The objectives of present study were to characterize the peptides which were isolated from Korean fermented soybean paste, chungkukjang, and to determine their antioxidant activities. Four fractions were collected from the methanol extract of chungkukjang by using a recycling preparative HPLC. Among fractions, Fr-2 was identified to be highly potent free radical scavenging activity in the assay of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and nitroblue tetrazolium(NBT)-reduction inhibition. Base on antioxidant effects, fraction Fr-2 was employed for the refraction with a prep-column and separated into five fractions of which two fractions were identified to have higher antioxidant activity. To confirm the amino acid constituents of antioxidant fractions Fr-2-2 and Fr-2-3 were analyzed, and eight kinds of amino acids such as aspartic acid, threonine, serine, glutamic acid, glycine, lysine, histidine, and arginine were identified as the constituent amino acids. Antioxidant activities of the separated peptides were further assessed cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT), and fluorescence-activated cell sorting (FACS) analysis of H4IIE cells treated with hydrogen peroxide (H 2 O 2 ). Chungkukjang peptides have shown their ability to protect H4IIE rat hepatoma cells against H 2 O 2 -induced oxidative stress by concentration and time-dependent manner. Therefore, These results indicated that fermented soybean paste chungkukjang will be promoted the antioxidant and radical scavenging activities, and beneficial for health. The antioxidant peptide fractions Fr-2-2 and Fr-2-3 were denominated as P-NICS-1 and P-NICS-2, respectively. However, further studies were required to clarify their amino acid sequences and molecular properties, and physiological significances.

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Analysis of phenolic compounds and antioxidant activity with H4IIE cells of three different rice grain varieties

Cited by 24 publications

References 20 publications

Study of chemical composition and volatile compounds along with in-vitro assay of antioxidant activity of two medicinal rice varieties: Karungkuravai and Mappilai samba

Study of chemical composition and volatile compounds along with in-vitro assay of antioxidant activity of two medicinal rice varieties: Karungkuravai and Mappilai samba

Quality and Grain Yield Attributes of Rwandan Rice (Oryza sativaL.) Cultivars Grown in a Biotron Applying Two NPK Levels

Evaluation of Antioxidant Activities of Peptides Isolated from Korean Fermented Soybean Paste, Chungkukjang

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