Analysis of phenolic compounds by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry in senescent and water-stressed tobacco

Torras-Claveria, Laura; Jáuregui, Olga; Codina, Carles; Tiburcio, Antonio F.; Bastida, Jaume; Viladomat, Françesc

doi:10.1016/j.plantsci.2011.02.009

Cited by 67 publications

(52 citation statements)

References 60 publications

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“…Phenolic compounds such as phenolic acids and flavonoids have been found to be the most widespread substantial groups of plant secondary metabolites produced from the shikimate-phenylpropanoid biosynthetic pathway [6,7]. These molecules have been described as markers of biotic and abiotic stress tolerance in plants [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Markham et al [16] reported that, in different UV-B light levels, C-glycosylflavone contents increasingly appeared in a UV-tolerant rice cultivar but were non-existent in a susceptible cultivar. Torras-Claveria et al [6] identified 20 phenolic compounds in both watered and water deficit stressed tobacco plants, and most of these compounds were detected to increase more in water-stressed plants. Similarly, the observed enhancement of total contents of phenolics, flavonoids, and anthocyanins, and schaftosides, in response to drought in wheat leaves, were demonstrated by Ma et al [7].…”

Section: Introductionmentioning

confidence: 99%

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Involvement of Secondary Metabolites in Response to Drought Stress of Rice (Oryza sativa L.)

Quan

Anh²,

Khang³

et al. 2016

Agriculture

104

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Abstract:In this study, responses of rice under drought stress correlating with changes in chemical compositions were examined. Among 20 studied rice cultivars, Q8 was the most tolerant, whereas Q2 was the most susceptible to drought. Total phenols, total flavonoids, and antioxidant activities, and their accumulation in water deficit conditions were proportional to drought resistance levels of rice. In detail, total phenols and total flavonoids in Q8 (65.3 mg gallic acid equivalent (GAE) and 37.8 mg rutin equivalent (RE) were significantly higher than Q2 (33.9 mg GAE/g and 27.4 mg RE/g, respectively) in both control and drought stress groups. Similarly, the antioxidant activities including DPPH radical scavenging, β-carotene bleaching, and lipid peroxidation inhibition in Q8 were also higher than in Q2, and markedly increased in drought stress. In general, contents of individual phenolic acids in Q8 were higher than Q2, and they were significantly increased in drought stress to much greater extents than in Q2. However, p-hydroxybenzoic acid was found uniquely in Q8 cultivars. In addition, only vanillic acid was found in water deficit stress in both drought resistant and susceptible rice, suggesting that this phenolic acid, together with p-hydroxybenzoic acid, may play a key role in drought-tolerance mechanisms of rice. The use of vanillic acid and p-hyroxybenzoic acid, and their derivatives, may be useful to protect rice production against water shortage stress.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Involvement of Secondary Metabolites in Response to Drought Stress of Rice (Oryza sativa L.)

Quan

Anh²,

Khang³

et al. 2016

Agriculture

104

View full text Add to dashboard Cite

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“…Additionally, it was found that extraction temperatures above 60 °C should be avoided and degassed extraction solvents should be used with pressurized liquid extraction (PLE) due to the chemical reactivity of the phenolics (17,18). The analysis of polyphenols from botanical sources, particularly solanaceous plants like tobacco, using mass spectrometry has been the subject of a significant amount of research and ESI-MS/MS has been used to generate fragmentation data on major and minor polyphenols found in plant extracts (19)(20)(21)(22)(23)(24)(25)(26). Similarities in the fragmentation of the three isomers of chlorogenic acid are well documented (19)(20)(21)(22) and emphasize the need for the optimized chromatographic separation presented here to both distinguish and quantify these compounds in tobacco extracts.…”

Section: Introductionmentioning

confidence: 99%

“…The analysis of polyphenols from botanical sources, particularly solanaceous plants like tobacco, using mass spectrometry has been the subject of a significant amount of research and ESI-MS/MS has been used to generate fragmentation data on major and minor polyphenols found in plant extracts (19)(20)(21)(22)(23)(24)(25)(26). Similarities in the fragmentation of the three isomers of chlorogenic acid are well documented (19)(20)(21)(22) and emphasize the need for the optimized chromatographic separation presented here to both distinguish and quantify these compounds in tobacco extracts. In the work discussed here, a validated method for the analysis of polyphenols in cured tobacco leaf as well as tobacco fill material from cigars and cigarettes using PLE and Ultra Performance Liquid Chromatography with Electrospray Ionization - Tandem Considering that the mandate of the TTB is to apply tax rates to finished tobacco products, the scope of application of this analytical method should be relatively straightforward.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Polyphenols of Tobacco Using Pressurized Liquid Extraction (PLE) and Ultra Performance Liquid Chromatography With Electrospray Ionization – Tandem Mass Spectometric Detection (UPLC-ESI-MS/MS)

Shifflett

Watson

McNally

et al. 2017

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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SUMMARYPolyphenols are chemicals found in tobacco that are affected by the method used to cure the leaf and, as a result, can be useful in the characterization of tobacco products. The purpose of this work was to develop an analytical method to investigate the levels of six polyphenols found in tobacco leaves and tobacco products: 3-O-caffeoylquinic acid (chlorogenic acid), 4-O-caffeoylquinic acid (cryptochlorogenic acid), 5-O-caffeoylquinic acid (neochlorogenic acid), kaempferol 3-O-rutinoside (nicotiflorin), quercetin 3-O-rutinoside (rutin), and 6-methoxy-7-hydroxycoumarin (scopoletin). Extraction conditions for sample preparation using PLE and instrument conditions for analysis by UPLC-MS/MS were optimized and validated. Results from the analysis of 30 cured tobacco leaves are presented and discussed in the context of each curing method represented.Results from the analysis of various tobacco products are also presented and trends observed across product types are discussed in the context of the applicability of the validated method. Total polyphenol levels for flue-cured, Oriental, and air-cured leaves were determined to be in the ranges of 18-41 mg/g, 5-27 mg/g, and 0.5-3 mg/g respectively. Similarly, cigarette polyphenol levels were found in the range of 4-16 mg/g and cigar polyphenol levels were less than 1.5 mg/g. The trends observed in the results for the tobacco leaf samples are consistent with expectations regarding the fate of polyphenols under the conditions commonly used in curing procedures. The results for the tobacco products demonstrate that the validated method can be used to study polyphenol content in cigarettes and a variety of cigar types including pipe tobacco cigars. [Beitr. Tabakforsch. Int. 27 (2017) 195-207]

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“…Currently, various analytical techniques used for separation and identification of glycosides in tobaccos include enzymatic hydrolysis or acid hydrolysis coupled with gas chromatography-mass spectrometry (GC-MS) method, 9,[14][15][16][17] and liquid chromatography-mass spectrometry (LC-MS) or liquid chromatography-tandem mass spectrometry (LC/MS n ) method. [18][19][20][21] However, those methods above are indirect because they need comparison with standard references and literature data. Therefore it is desirable to develop a rapid and sensitive method for identifying and characterizing the known or new structures.…”

Section: Introductionmentioning

confidence: 99%

Identification of Glycoside Compounds from Tobacco by High Performance Liquid Chromatography/Electrospray Ionization Linear Ion-Trap Tandem Mass Spectrometry Coupled with Electrospray Ionization Orbitrap Mass Spectrometry

Jia¹,

Zhu²,

Zhang³

et al. 2016

J. Braz. Chem. Soc.

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In order to comprehensively screen and identify the glycoside compounds in tobacco, a simple, rapid and sensitive method of high performance liquid chromatography/electrospray ionization linear ion-trap tandem mass spectrometry (HPLC-ESI-LIT/MS n ) coupled with electrospray ionization orbitrap mass spectrometry (ESI-Orbitrap-MS) was developed for the first time. As a result, twenty-two glycoside compounds, including eleven alcoholic glycosides, eight phenolic glycosides, two ester glycosides and an indole glycoside, were reliably identified from tobacco with high mass accuracy (within 5 mDa). Among them, four compounds were confirmed as novel molecules and other four compounds, as far as we know, were not reported previously in tobacco. This study provided a useful tool to identify the new structures of glycoside compounds in natural products, especially when there were no reference compounds available.

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Analysis of phenolic compounds by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry in senescent and water-stressed tobacco

Cited by 67 publications

References 60 publications

Involvement of Secondary Metabolites in Response to Drought Stress of Rice (Oryza sativa L.)

Involvement of Secondary Metabolites in Response to Drought Stress of Rice (Oryza sativa L.)

Analysis of the Polyphenols of Tobacco Using Pressurized Liquid Extraction (PLE) and Ultra Performance Liquid Chromatography With Electrospray Ionization – Tandem Mass Spectometric Detection (UPLC-ESI-MS/MS)

Identification of Glycoside Compounds from Tobacco by High Performance Liquid Chromatography/Electrospray Ionization Linear Ion-Trap Tandem Mass Spectrometry Coupled with Electrospray Ionization Orbitrap Mass Spectrometry

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