Analysis of proteinaceous binding media used in Tang Dynasty polychrome pottery by MALDI-TOF-MS

Yan, Hong; An, Jing; Zhou, Taofa; Li, Yu Hu

doi:10.1007/s11434-013-5781-7

Cited by 8 publications

(7 citation statements)

References 12 publications

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“…Their accurate identification is crucial for studying the context of objects and fundamental to improve the conservation practices. Proteomics approaches are often employed for the identification of protein binders in cultural heritage objects, in particular in historical paints, frescoes, , and polychrome pottery . Reliable protein extraction and identification from old-age parchment documents had already been performed by sampling tiny fragments, while parchment proteins were noninvasively extracted by using an electrostatic charge generated by the gentle rubbing of a PVC eraser .…”

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confidence: 99%

“…Proteomics approaches are often employed for the identification of protein binders in cultural heritage objects, in particular in historical paints, 3 frescoes, 4,5 and polychrome pottery. 6 Reliable protein extraction and identification from old-age parchment docu- ments had already been performed by sampling tiny fragments, 7 while parchment proteins were noninvasively extracted by using an electrostatic charge generated by the gentle rubbing of a PVC eraser. 8 The in situ analysis of proteins has been investigated using desorption electrospray ionization-mass spectrometry (DESI-MS), thus obtaining significant results and good preservation of the protein material.…”

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Method for Noninvasive Analysis of Proteins and Small Molecules from Ancient Objects

et al. 2017

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Proteins and small molecules from ancient objects and cultural heritage can provide key information and contribute to study the context of objects and artists. However, all present-day protocols and strategies for the analysis of ancient samples are often invasive and require microsampling. Here, we present a new method for the noninvasive analysis of proteins and small molecules: the technique uses a special ethyl-vinyl acetate film functionalized with strong cation/anion exchange and C resins, for interacting with both proteins and small molecules present on the surface of the objects, followed by LC-MS/MS analysis. The new method was fully validated for the determination of both proteins and small molecules on several types of supports, showing excellent analytical performances such as, for example, R of the calibration curve of 0.98 and 0.99 for proteins and small molecules, low but very repeatable recoveries, particularly adequate for investigations on precious ancient samples that must not be altered by the analytical procedure. ESEM images and LED multispectral imaging confirmed that no damages or alterations occurred onto the support surfaces and no residues were left from the extractive film. Finally, the new method was applied for the characterization of the binders of a historical fresco of the XVI century from the Flemish painter Paul Brill and of a recently discovered fresco from Isidoro Bianchi (XVII century). Moreover the method was employed for the identification of the colorant used by Pietro Gallo (XIV century) on a wood panel. The method here reported can be easily applied to any other research on ancient precious objects and cultural heritage, since it does not require microsampling and the proteins/small molecules extraction can be performed directly in situ, leaving the object unchanged and intact.

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Method for Noninvasive Analysis of Proteins and Small Molecules from Ancient Objects

et al. 2017

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“…TFA is shown to be a promising extraction agent in the identication of protein. 26,31 Compared to other methods, 32,33 TFA-ultrasound-assisted extraction can provide the total proteins, it has such advantages as simple and rapid operation, good repeatability, low consumption chemical reagents. It can minimize the sample loss and avoid the risk of sample contaminant result from in the digestion or derivatisation.…”

Section: Single-factor Optimization Of Protein Extraction Conditionmentioning

confidence: 99%

Optimization of ultrasound-assisted extraction of protein from egg white using response surface methodology (RSM) and its proteomic study by MALDI-TOF-MS

Yan

Fan

2014

RSC Adv.

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The presented work proposes an approach based on proteomic techniques to identify proteins in egg white. Response surface methodology (RSM) with Box-Behnken design (BBD) was used to optimize the ultrasound-assisted extraction process of proteins from the egg white. The effects of extraction parameters, including trifluoroacetic acid (TFA) concentration, ultrasonic power and ultrasonic time on the extraction process of proteins were evaluated. The optimized extraction parameters were trifluoroacetic acid (TFA) concentration, 0.53% (v/v); ultrasonic power, 50% and ultrasonic time, 40 min. The effects of three factors (trypsin volume, pH of tryptic digestion and incubation time) on the tryptic digestion process of extracted proteins were investigated. The results showed that the optimized tryptic digestion conditions were as follows: volume of 10 mg mL À1 trypsin, 8 mL; pH, 7.0 and incubation time, 16 h. Under the optimized conditions, the peptide mixture was analysed using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). The methodology was successfully applied to free-range and barn-raised chicken egg white for protein identification. The results showed that 23 proteins and 29 proteins were identified in free-range chicken and barn-raised chicken egg white, respectively. Among them, the collagen peptides were found in egg white for the first time.

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“…Reagents routinely used to extract proteinaceous residues from paint samples include trifluoroacetic acid (TFA) [14,22,23], Urea [5,24,25], guanidine hydrochloride (GuHCl) [26,27], ammonia [14,28,29] and ammonium bicarbonate (AMBIC) [30][31][32]. Acetic acid is also a potential candidate, as it is commonly used for biological collagen extraction [33,34], paleontology [35,36] and food industry [37].…”

Section: Introductionmentioning

confidence: 99%

A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings

Zhu

Yang

et al. 2021

Herit Sci

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In this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These agents were used to extract the residual proteins in the non-aged and thermal aged samples. The protein extraction efficiencies of different extracting agents were quantitatively determined by bicinchoninic acid (BCA) method, and then processed by multivariate analysis of variance (MANOVA). The impact of the extraction process on the protein structure was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet absorption spectrum (UV) and circular dichroism (CD). The results showed that, for both non-aged and aged samples, the extraction efficiency of 2 M guanidine hydrochloride (GuHCl) was significantly higher than the other five agents, with less damage to the protein structure during the extraction process.

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Analysis of proteinaceous binding media used in Tang Dynasty polychrome pottery by MALDI-TOF-MS

Cited by 8 publications

References 12 publications

Method for Noninvasive Analysis of Proteins and Small Molecules from Ancient Objects

Method for Noninvasive Analysis of Proteins and Small Molecules from Ancient Objects

Optimization of ultrasound-assisted extraction of protein from egg white using response surface methodology (RSM) and its proteomic study by MALDI-TOF-MS

A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings

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