Analysis of protocols for application of an LHRH analog for induced final maturation and ovulation of female pacu (Piaractus mesopotamicus Holmberg 1887)

Carolsfeld, Joachim; Ramos, Sergio Moreira; Ormanezi, R; Gomes, João Henrique; Barbosa, José Milton; Harvey, B.

doi:10.1016/0044-8486(88)90085-3

Cited by 12 publications

(9 citation statements)

References 4 publications

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“…In this context, some previous studies have reported that the use of synthetic products causes ovulation in native migratory fish but fails to provide viable embryos, probably due to treatment toxicity (Pereira et al, 2017). The potential use of GnRH in tropical migratory species was initially showed by Carolsfeld et al (1988) showing that LHRH ethylamide (100 µg/kg in single dose) can provoke final maturation and ovulation in Piaractus mesopotamicus, another tropical reophilic fish. However, the use of GnRH or other synthetic products was not continued due to inconsistent results obtained in later studies with the same species (Paulino et al, 2011), as well as for other South American reophilic species (Ramos et al, 1997;Carneiro and Mikos, 2008;Acuña and Rangel, 2009;Paulino et al, 2011;Pereira et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Souza

Kuradomi

Rodrigues³

2020

Anim Reprod

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How to cite: Souza TG, Kuradomi RY, Rodrigues SM, Batlouni SR. Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract. Anim Reprod. 2020;17(1):e20190078. AbstractBreeding technology is of utmost importance for reproduction of wild fish in captivity for the reintroduction and selective breeding programs purposes. The main challenge is that when applied to wild undomesticated specimens, conventional protocols often cause breeders and/or embryo mortality and spawning failure. In this study, we evaluated the reproductive performance of wild Leporinus friderici, a great importance fish for subsistence fishing in South American rivers, applying conventional and lower-dose hormonal therapies by means of two consecutive experiments. In the first, a conventional (0.5 and 5.5 mg/kg) and a lower carp pituitary extract (CPE) dose (0.5 and 1.0 mg/kg) were applied. In the second, a conventional mammalian GnRH analogue associated with metoclopramide (mGnRHa + MET) (40 µg mGnRHa + 20 mg MET/kg) and a lower dose (4 μg mGnRHa + 2 mg MET/kg and 8 µg + 4 mg of mGnRHa + MET/kg) were applied. Ovulation was observed in all treatments, however, only lower CPE protocol provided viable embryos. High levels of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and 17β estradiol (E 2 ) detected in conventional, but not in lower CPE dose, at ovulation, might be associated to the mortality of the embryos. The use of lower CPE dose applied here was the best way to obtain L. friderici viable embryos. These results directly contribute to the knowledge about poorly explored effects of reproductive management and hormonal therapies in wild-type breeders, showing that the use of reduced doses may be an alternative to reproductive success.

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Section: Discussionmentioning

confidence: 99%

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Souza

Kuradomi

Rodrigues³

2020

Anim Reprod

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“…According to the literature (Table 3), Characiformes females treated with a single or double dose of GnRH do not spawn ( B. cephalus ; Ramos et al ., 1997) or spawn only after very high doses of GnRH ( P. mesopotamicus ; Carolsfeld et al ., 1988). When GnRH was injected after PE treatment, none out of nine Brycon siebenthalae (Pardo-Carrasco et al ., 2002), five out of eight P. mesopotamicus (Carolsfeld et al ., 1988) and four out of six P. mesopotamicus (Zaniboni-Filho, 1995) females were reported to have spawned. However, the number of females per treatment was too small to draw any conclusion from these studies.…”

Section: Discussionmentioning

confidence: 99%

“…When possible, osmolality and pH values were compared between semen and seminal plasma of the same males. Osmolality was always higher in semen (286 mOsm/kg in Ovaprim TM -treated and 281 mOsm/kg in PE-treated males) compared with seminal plasma (278 mOsm/kg in Ovaprim TM -treated Carolsfeld et al, 1988 • (GnRH + domp) d = (10 μg + 5 mg) and 257 mOsm/kg in PE-treated males). In comparison, the pH was always lower in semen (pH 7.4 in both Ovaprim TM -treated and PE-treated males) compared with seminal plasma (pH 7.9 in Ovaprim TM -treated and 8.0 in PE-treated males).…”

Section: Experiments 2 Sperm Qualitymentioning

confidence: 93%

“…In Characiformes fish, however, the use of GnRH (or luteinizing hormone releasing hormone (LHRH)), as the spawning or spermiation inducer has been tested in some species, but with little or no success. In Brycon cephalus , none of the 27 GnRH-treated females spawned (Ramos et al ., 1997), while in Piaractus mesopotamicus only eight out of 17 GnRH-treated females spawned (Carolsfeld et al ., 1988). Furthermore, Brycon amazonicus males treated with GnRH possessed lower semen volume, higher sperm concentration and similar sperm motility rate compared with PE-treated males (Pardo-Carrasco et al ., 2006).…”

Section: Introductionmentioning

confidence: 99%

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Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment

Viveiros

Gonçalves

Chiacchio

et al. 2013

Zygote

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The efficiency of Ovaprim™ salmon gonadotropin-releasing hormone agonist (GnRHa) and dopamine antagonist on the induction of spawning and spermiation in Prochilodus lineatus in comparison with the commonly used method using pituitary extract (PE) was evaluated. Females received PE at 0.5 + 5.0 mg/kg and Ovaprim™ at 0.05 + 0.45 ml/kg or at 0.125 + 0.375 ml/kg. All males received a first dose of PE at 0.4 mg/kg and then PE at 4.0 mg/kg or Ovaprim™ at 0.25 ml/kg. Oocyte, egg, larvae and sperm quality were evaluated. All females spawned and oocyte, egg and larvae quality were similar between Ovaprim™-treated (both doses) and PE-treated females. Data from females were pooled and the mean values were: 242 g ova weight, 15% ova index, 1209 oocytes/g ova, 284,539 oocytes/female, 183 oocytes/g body weight, 1.18 mm oocyte diameter, 49% fertilization rate, 43% hatching rate and 89% normal larvae. Sperm quality was similar between Ovaprim™-treated and PE-treated males. Data from males were pooled and the mean values of semen were: volume of 3.0 ml, 14.9 × 109 sperm/ml, osmolality of 283 mOsm/kg, pH of 7.4, 71% motile sperm, 217 μm/s curvilinear velocity, 102 μm/s straight-line velocity and 189 μm/s average path velocity. Ovaprim™ treatment can be used for commercial reproduction of P. lineatus, without any loss of gamete quality in comparison with PE treatment.

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“…Also various investigators have examined the effect of pituitary dosage administered (Ufodike et al, 1986). Zonneveled et al, (1988) and Carolfeld et al, (1988) had determined the optimum dosage required to ensure no hormonal wastage.…”

Section: Introductionmentioning

confidence: 99%

The Effect Of Homoplastic Pituitary Injection Overdose On Induced Spawning Of African Catfish <i>Carias gariepinus</i>, Burchell 1822

Orji¹

2008

Anim. Res. Int.

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Twelve pairs of male and female African catfish, Clarias gariepinus broodfish were monthly treated with graded doses of crude homoplastic pituitary injection. Different sets o pairs were used for each month, after certifying their gonadal maturity fitness for induced breeding. The first two pairs of spawners received one pituitary gland (3.8-5.7 mg) each from donors having equivalent body weight. The second two pairs received two glands (7.2-11 3 g), the third two pairs received three g ands (10.1-16.2 mg) the fourth two pa rs received four glands (13.5-22.2 mg) and the fifth two pairs received five g ands (17 7-27.5 mg) the sixth two pairs (contro) were not injected Each spawning pair was kept in concrete spawning tank for 24 hours for natural spawning to take place. Administration of one pitui ary injection fai ed to induce spawning, two and three glands yielded optimum results. Four and five yielded good spawning but all the hatchlings died after hatching. Death may be attributed to over secretion of thyroxin, thus leading to faculty vitellogenesis.

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Analysis of protocols for application of an LHRH analog for induced final maturation and ovulation of female pacu (Piaractus mesopotamicus Holmberg 1887)

Cited by 12 publications

References 4 publications

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment

The Effect Of Homoplastic Pituitary Injection Overdose On Induced Spawning Of African Catfish <i>Carias gariepinus</i>, Burchell 1822

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