Analysis of RegA, a pathway-specific regulator of the friulimicin biosynthesis in Actinoplanes friuliensis

Nolden, Susanne; Wagner, Nina; Biener, Richard; Schwartz, Dirk

doi:10.1016/j.jbiotec.2008.12.013

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…This correlates with the onset of friulimicin production (Nolden et al, 2009). The analysis of pstA under the control of the ermE* promoter showed no significant differences in the transcription rate during the cultivation.…”

Section: Comparison Of Heterologous and Homologous Promoter Strengthsmentioning

confidence: 68%

“…To characterize the function of friulimicin biosynthetic genes by gene inactivation, the aprP-resistance cassette was used for the generation of gene replacement and gene insertion mutants of A. friuliensis (Heinzelmann et al, 2003;Müller et al, 2007;Nolden et al, 2009). For construction of this cassette, the ermE* promoter was selected in order to ensure transcription of genes downstream of the gene interruption site.…”

Section: Comparison Of Heterologous and Homologous Promoter Strengthsmentioning

confidence: 99%

“…In order to compare the strength of the heterologous ermE* promoter and the homologous promoter of the biosynthetic gene cluster, the transcriptional rate of the biosynthetic gene pstA was analysed by real-time RT-PCR in the wild-type and the mutant AF-O4-23. For transcription analysis total RNA was prepared from both strains after growth for 24, 48, 72 and 96 h in seed medium (Aretz et al, 2000) as described by Nolden et al (2009). Transcription rates were analysed by real-time RT-PCR using the iCycler iQ real-time PCR detection system (Bio-Rad, Hercules, USA).…”

Section: Comparison Of Heterologous and Homologous Promoter Strengthsmentioning

confidence: 99%

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Comparative analysis of transcriptional activities of heterologous promoters in the rare actinomycete Actinoplanes friuliensis

Wagner

Oßwald

Biener

et al. 2009

Journal of Biotechnology

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Section: Comparison Of Heterologous and Homologous Promoter Strengthsmentioning

confidence: 68%

Section: Comparison Of Heterologous and Homologous Promoter Strengthsmentioning

confidence: 99%

Section: Comparison Of Heterologous and Homologous Promoter Strengthsmentioning

confidence: 99%

See 1 more Smart Citation

Comparative analysis of transcriptional activities of heterologous promoters in the rare actinomycete Actinoplanes friuliensis

Wagner

Oßwald

Biener

et al. 2009

Journal of Biotechnology

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“…Their corresponding counterparts RegA, RegB, RegC and RegD were identified in the friulimicin cluster (Muller et al, 2007). RegA was demonstrated as a pathway-specific regulator of friulimicin biosynthesis in Actinoplanes friuliensis (Nolden et al, 2009). Inactivation of RegA resulted in abolishment of friulimicin production in the mutant.…”

Section: Resultsmentioning

confidence: 99%

Molecular cloning and identification of the laspartomycin biosynthetic gene cluster from Streptomyces viridochromogenes

Wang

Chen

Shen

et al. 2011

Gene

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The biosynthetic gene cluster for laspartomycins, a family of 11 amino acid peptide antibiotics, has been cloned and sequenced from Streptomyces viridochromogenes ATCC 29814. Annotation of a segment of 88912 bp of S. viridochromogenes genomic sequence revealed the putative las cluster and its flanking regions which harbor 43 open reading frames. The lpm cluster, which spans approximately 60 kb, consists of 21 open reading frames. Those include four NRPS genes (lpmA/orf18, lpmB/orf25, lpmC/orf26 and lpmD/orf27), four genes (orfs 21, 22, 24 and 29) involved in the lipid tail biosynthesis and attachment, four regulatory genes (orfs 13, 19, 32 and 33) and three putative exporters or self-resistance genes (orfs 14, 20 and 30). In addition, the gene involved in the biosynthesis of the nonproteinogenic amino acid Pip was also identified in the lpm cluster while the genes necessary for the biosynthesis of the rare residue diaminopropionic acid (Dap) were found to reside elsewhere on the chromosome. Interestingly, the dabA, dabB and dabC genes predicted to code for the biosynthesis of the unusual amino acid diaminobutyric acid (Dab) are organized into the lpm cluster even though the Dab residue was not found in the laspartomycins. Disruption of the NRPS lpmC gene completely abolished laspartomycin production in the corresponding mutant strain. These findings will allow molecular engineering and combinatorial biosynthesis approaches to expand the structural diversity of the amphomycin-group peptide antibiotics including the laspartomycins and friulimicins.

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“…In contrast to LAL, RegA is a small protein (254 aa) with a helix-turn-helix motif of LuxR type at the C-terminal end. Deletion of regA results in friulimicin nonproducing mutants, indicating that RegA positively regulates friulimicin biosynthesis [Nolden et al, 2009]. Gene expression analysis shows that the expression of most of friumicin biosynthetic genes is impaired in the mutant, while the expression of regA is enhanced, indicating that RegA negatively controls its own expression.…”

Section: Pathway Specific Regulatory Genesmentioning

confidence: 99%

Regulation mechanisms underlying the biosynthesis of daptomycin and related lipopeptides

Liao

Shi

Xie

2012

J of Cellular Biochemistry

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Daptomycin is a lipopeptide antibiotics used to treat Gram-positive pathogens infections, including drug-resistant strains. In-depth exploration of its biosynthesis and regulation is crucial for metabolic engineering improvement of this ever-increasing important antibiotic. The past years have witnessed the significant progresses in the understanding of the molecular mechanisms underlying the biosynthesis and regulation of daptomycin. This information was updated in our review, with special focus on the regulatory network integrating a wide variety of physiological and environmental inputs. This should provide novel insight into the regulatory mechanism of biosynthesis of daptomycin and nodes for strain improvement to increase the yields of daptomycin.

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Analysis of RegA, a pathway-specific regulator of the friulimicin biosynthesis in Actinoplanes friuliensis

Cited by 7 publications

References 34 publications

Comparative analysis of transcriptional activities of heterologous promoters in the rare actinomycete Actinoplanes friuliensis

Comparative analysis of transcriptional activities of heterologous promoters in the rare actinomycete Actinoplanes friuliensis

Molecular cloning and identification of the laspartomycin biosynthetic gene cluster from Streptomyces viridochromogenes

Regulation mechanisms underlying the biosynthesis of daptomycin and related lipopeptides

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